Metabolic stability and determination of cytochrome P450 isoenzymes' contribution to the metabolism of medetomidine in dog liver microsomes

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Marie-Claude Duhamel ◽  
Éric Troncy ◽  
Francis Beaudry
Keyword(s):  
Cytochrome P450 ◽  
Liver Microsomes ◽  
Metabolic Stability ◽  
Dog Liver

A New Cytochrome P450 Form Belonging to the CYP2D in Dog Liver Microsomes: Purification, cDNA Cloning, and Enzyme Characterization

1995 ◽  
Vol 319 (2) ◽  
pp. 372-382 ◽  
Author(s):  
K. Sakamoto ◽  
S. Kirita ◽  
T. Baba ◽  
Y. Nakamura ◽  
Y. Yamazoe ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cdna Cloning ◽  
Liver Microsomes ◽  
Enzyme Characterization ◽  
Dog Liver

scholarly journals Eco-Friendly, Simple, Fast, and Sensitive UPLC-MS/MS Method for Determination of Pexidartinib in Plasma and Its Application to Metabolic Stability

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 297
Author(s):  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Yousif A. Asiri ◽  
Gamal A. E. Mostafa ◽  
Ahmed Y. A. Sayed
Keyword(s):  
Formic Acid ◽  
Method Validation ◽  
Multiple Reaction Monitoring ◽  
Liver Microsomes ◽  
Internal Standard ◽  
Metabolic Stability ◽  
Methyl Tert Butyl Ether ◽  
Butyl Ether ◽  
Limit Of Quantitation

Pexidartinib is the first drug approved by the U.S. Food and Drug Administration specifically to treat the rare joint tumor tenosynovial giant cell tumor. In the current study, a validated, selective, and sensitive UPLC-MS/MS assay was developed for the quantitative determination of pexidartinib in plasma samples using gifitinib as an internal standard (IS). Pexidartinib and IS were extracted by liquid-liquid extraction using methyl tert-butyl ether and separated on an acquity BEH C18 column kept at 40 °C using a mobile phase of 0.1% formic acid in acetonitrile: 0.1% formic acid in de-ionized water (70:30). The flow rate was 0.25 mL/min. Multiple reaction monitoring (MRM) was operated in electrospray (ESI)-positive mode at the ion transition of 418.06 > 165.0 for the analyte and 447.09 > 128.0 for the IS. FDA guidance for bioanalytical method validation was followed in method validation. The linearity of the established UPLC-MS/MS assay ranged from 0.5 to 1000 ng/mL with r > 0.999 with a limit of quantitation of 0.5 ng/mL. Moreover, the metabolic stability of pexidartinib in liver microsomes was estimated.

High performance liquid chromatography determination of cytochrome P450 1A and 2C activities in bovine liver microsomes

2010 ◽  
Vol 183 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Sara Pegolo ◽  
Roberta Merlanti ◽  
Mery Giantin ◽  
Mauro Dacasto ◽  
Clara Montesissa ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Cytochrome P450 ◽  
Liquid Chromatography ◽  
High Performance ◽  
Liver Microsomes ◽  
Bovine Liver ◽  
Cytochrome P450 1A
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