Analytical method development and determination of hydrocortisone acetate and fusidic acid simultaneously in cream formulation, by reversed‐phase HPLC

2020 ◽  
Author(s):  
Nawaz Rasool ◽  
Qudsia Kanwal ◽  
Muhammad Waseem ◽  
Mehrunnisa ◽  
Muhammad Irfan Khan
Keyword(s):  
Fusidic Acid ◽  
Analytical Method ◽  
Method Development ◽  
Reversed Phase ◽  
Hydrocortisone Acetate ◽  
Reversed Phase Hplc ◽  
Cream Formulation

Development of an analytical method by defining a design space: a case study of saponin determination for Panax notoginseng extracts

2016 ◽  
Vol 8 (10) ◽  
pp. 2282-2289 ◽  
Author(s):  
Xingchu Gong ◽  
Ying Zhang ◽  
Huali Chen ◽  
Teng Chen ◽  
Jianyang Pan ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Design Space ◽  
Panax Notoginseng ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Space Approach

A reversed-phase HPLC-UV method was developed using a design space approach for the simultaneous determination of five saponins in the extracts of Panax notoginseng, which were notoginsenoside R1, ginsenosides Rg1, Re, Rb1, and Rd.

Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

2014 ◽  
Vol 26 (1) ◽  
pp. 57-66 ◽  
Author(s):  
M. Nawaz ◽  
M. S. Arayne ◽  
N. Sultana ◽  
A. Haider ◽  
S. Hisaindee
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Fusidic Acid ◽  
Reversed Phase ◽  
Reversed Phase Hplc

scholarly journals ANALYTICAL METHOD DEVELOPMENT AND METHOD VALIDATION FOR DETERMINATION ASSAY AND CONTENT UNIFORMITY OF LEVONORGESTREL BY REVERSED-PHASE HIGHPERFORMANCE LIQUID CHROMATOGRAPHY

2020 ◽  
pp. 101-107
Author(s):  
Vikas kumar Pal ◽  
Yogendra Pal
Keyword(s):  
Liquid Chromatography ◽  
Analytical Method ◽  
Method Development ◽  
Reversed Phase ◽  
Hplc Method ◽  
Acetonitrile Solution ◽  
Simultaneous Estimation ◽  
Content Uniformity ◽  
Rp Hplc

Object: The main objective of the complete study is to develop a new method and also to validate the developed method for the determination of Assay and Content Uniformity of Levonorgestrel by reverse-phase high performance liquid chromatography (RP-HPLC). Methods: RP-HPLC method was developed for simultaneous estimation of levonorgestrel using Hypersil ODS, 125 mm×4.6 mm×5 μm C8 column with a mixture of water, and acetonitrile solution with a ratio of 50:50 as a mobile phase at a flow rate of 1.3 mL/min with a detection of quantification wavelength of 242 nm. Method was selected after calculating system suitability and validated as per International Conference on Harmonization (ICH) guidelines. Results: The developed analytical method parameters found within the limits as given in ICH and USP Guidelines and the total chromatographic analysis time per sample was 8 min with Levonorgestrel Eluting with retention time of 4.479, 4.479, and 4.467 min, respectively. The validated HPLC method was successfully applied for the determination of dissolution of levonorgestrel tablets. Conclusion: The method is simple, precise, specific, and accurate. The newly developed method can be used for routine analysis of Levonorgestrel in tablet dosage form.

Determination of 5-hydroxymethyl-2-furaldehyde in royal jelly by a rapid reversed phase HPLC method

2013 ◽  
Vol 5 (19) ◽  
pp. 5010 ◽  
Author(s):  
Marco Ciulu ◽  
Roberta Farre ◽  
Ignazio Floris ◽  
Valeria M. Nurchi ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc

Simultaneous Determination of Ibuprofen and Pseudoephedrine Hydrochloride in Pharmaceutical Tablets by Reversed-Phase HPLC

2009 ◽  
Vol 42 (18) ◽  
pp. 2951-2961 ◽  
Author(s):  
Marie-Hélène Langlois ◽  
Philippe Dallet ◽  
Tina Kauss ◽  
Jean-Pierre Dubost
Keyword(s):  
Simultaneous Determination ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Pharmaceutical Tablets
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