Development and validation of a reversed-phase HPLC method for determination of nitrendipine in rat plasma: application to pharmacokinetic studies

2007 ◽  
Vol 21 (4) ◽  
pp. 363-368 ◽  
Author(s):  
Vinay Kumar Venishetty ◽  
Chandrasekar Durairaj ◽  
Ramakrishna Sistla ◽  
Madhusudhan Rao Yamsani ◽  
Prakash V. Diwan
Keyword(s):  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Pharmacokinetic Studies ◽  
Reversed Phase Hplc ◽  
Development And Validation

Ecofriendly chromatographic methods for determination of co-prescribed drugs, olanzapine and metformin, in rat plasma

Bioanalysis ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 597-613
Author(s):  
Aml A Emam ◽  
Neven M Habib ◽  
Hamada M Mahmoud ◽  
Nada S Abdelwhab ◽  
Maha M Abdelrahman
Keyword(s):  
Pharmaceutical Preparation ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hypoglycemic Agent ◽  
Chromatographic Methods ◽  
Rp Hplc ◽  
Development And Validation ◽  
Developing System

Background: Olanzapine (OLZ) is one of most recommended drugs for the treatment of schizophrenia while metformin (MET) is the most commonly used hypoglycemic agent. Aim: Development and validation of two green, sensitive and accurate chromatographic methods for the simultaneous determination of OLZ along with the co-prescribed, MET. Materials & methods: TLC-densitometric method with a developing system consisting of methylene chloride:methanol:ethyl acetate:triethylamine (4:4:5:0.1, by volume) and a reversed-phase (RP)-HPLC method where the chromatographic separation was performed using ethanol:water mixture (50: 50, v/v) as a mobile phase. Results: TLC-densitometric method had linearity over concentration ranges of 160–4000 ng/band for OLZ and 150–4500 ng/band for MET, while RP-HPLC method was linear and validated over concentration range of 300–20000 ng/ml for OLZ and MET. Conclusion: Pharmacokinetic study was successfully performed and suggested the possibility of co-administration of MET with OLZ and their further formulation in one pharmaceutical preparation to enhance patient’s compliance.

Development and Validation of a Reversed-Phase HPLC Method for the Determination of Deflazacort in Pharmaceutical Dosage Forms

2007 ◽  
Vol 65 (9-10) ◽  
pp. 591-594 ◽  
Author(s):  
G. M. Corrêa ◽  
L. P. Bellé ◽  
L. Bajerski ◽  
S. H. M. Borgmann ◽  
S. G. Cardoso
Keyword(s):  
Reversed Phase ◽  
Dosage Forms ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Pharmaceutical Dosage Forms ◽  
Development And Validation

scholarly journals Simultaneous HPLC determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon using HPLC

2020 ◽  
Vol 3 (1) ◽  
pp. 11-19
Author(s):  
Ngan Le Viet ◽  
Chi Le Dinh ◽  
Hong Anh Nguyen Thi ◽  
Ngoc Nguyen Bich ◽  
Phuong Lan Nguyen Thi ◽  
...  
Keyword(s):  
Room Temperature ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Hplc Method ◽  
Hydroxycinnamic Acid ◽  
Cinnamyl Alcohol ◽  
Reversed Phase Hplc ◽  
Sample Matrix ◽  
Development And Validation

The aim of the present study was the development and validation of a simple, precise and specific reversed phase HPLC method for the simultaneous determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon. The substances were extracted from the sample matrix with methanol, shaken at room temperature for 30 minutes and determined by HPLC-PDA on the following conditions: column C18 sunfire (250 × 4.6 mm, 5 µm), mobile phase 30mM ammonium acetate and methanol-acetonitrile (50:50) using gradient. The recovery > 90%, the repeatability has RSD < 7.3%, the LODs and LOQs of2-hydroxycinnamic acid, coumarin, cinnamaldehyd, cinnamyl alcohol, eugenol were 1 µg/g and 3.3 µg/g, respectively; the LODs and LOQs of cinnamic acid, cinnamyl acetate were 2 µg/g and 6.7 µg/g, respectively. The method has been applied to analyze the samples.

scholarly journals Development and Validation of a Reversed Phase HPLC Method for Determination of Anacardic Acids in Cashew (Anacardium occidentale) Nut Shell Liquid

2017 ◽  
Vol 56 (4) ◽  
pp. 300-306 ◽  
Author(s):  
Francisco Oiram Filho ◽  
Daniel Barbosa Alcântra ◽  
Tigressa Helena Soares Rodrigues ◽  
Lorena Mara Alexandre e Silva ◽  
Ebenezer de Oliveira Silva ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Hplc Method ◽  
Anacardium Occidentale ◽  
Reversed Phase Hplc ◽  
Anacardic Acids ◽  
Development And Validation
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