HPLC method for comparative study on tissue distribution in rat after oral administration of salvianolic acid B and phenolic acids fromSalvia miltiorrhiza

2007 ◽  
Vol 21 (10) ◽  
pp. 1052-1063 ◽  
Author(s):  
Man Xu ◽  
Gang Fu ◽  
Xue Qiao ◽  
Wan-Ying Wu ◽  
Hui Guo ◽  
...  
Keyword(s):  
Oral Administration ◽  
Comparative Study ◽  
Tissue Distribution ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Salvianolic Acid B ◽  
Salvianolic Acid

Analysis on the Stability of Total Phenolic Acids and Salvianolic Acid B from Salvia miltiorrhiza by HPLC and HPLC-MSn

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Man Xu ◽  
Jian Han ◽  
Hui-feng Li ◽  
Li Fan ◽  
Ai-hua Liu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Degradation Products ◽  
Biological Fluids ◽  
Salvia Miltiorrhiza ◽  
Degradation Pathway ◽  
Salvianolic Acid B ◽  
Total Phenolic ◽  
Salvianolic Acid ◽  
The Stability

The stability of salvianolic acid B and total phenolic acids from Salvia miltiorrhiza in water solutions at different temperatures, in buffered aqueous solutions at different pHs and in biological fluids, including simulated gastric and intestinal fluids, were investigated in vitro. The results showed that the degradation of salvianolic acid B was pH- and temperature-dependent. Furthermore, structures of the degradation products of salvianolic acid B and total phenolic acids were elucidated by liquid chromatography-electrospray ion trap mass spectrometry and analysis of the degraded solutions revealed seventeen degradation products. The possible degradation pathway of salvianolic acid B is proposed.

Quantitative determination and validation of four phenolic acids in Salvia Miltiorrhiza Bunge using 1H-NMR spectroscopy

2019 ◽  
Vol 16 ◽  
Author(s):  
Zhuoni Hou ◽  
Zongsuo Liang ◽  
Yuanyuan Li ◽  
Feng Su ◽  
Jipeng Chen ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
1H Nmr Spectroscopy ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Salvia Miltiorrhiza Bunge ◽  
Salvianolic Acid ◽  
Root Extracts

Background: Although chromatography and spectrometry based methods have been used to analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts. Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy. Method: Fresh SMB root were extracted using a 70% methanol aqueous solution and quantitatively analysed for danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B using 1H-NMR spectroscopy. Different internal standards were compared and the results were validated using high performance liquid chromatography. Results: The established method was accurate and precise with good recovery. The LOD and LOQ indicated the excellent sensitivity of the method. The robustness was testified by modification of four different parameters, and the differences among each parameter were all less than 2%. Conclusion: qNMR offers a fast, reliable and accurate method of identifying and quantifying danshensu, lithospermic acid, rosmarinic acid and salvianolic acid B in fresh SMB root extracts.

scholarly journals Comparative study of phenolic acids from underground parts of Rheum palmatum L., R. rhaponticum L. and R. undulatum L.

2011 ◽  
Vol 74 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Helena Danuta Smolarz ◽  
Ewa Medyńska
Keyword(s):  
Comparative Study ◽  
Ferulic Acid ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Rheum Palmatum ◽  
Rp Hplc ◽  
Rheum Undulatum

In three species of <em>Rheum</em> L. genus growing in Poland the composition of phenolic acids was determined. By 2D-TLC method the following acids were identified: ellagic, chlorogenic, gallic, protocatechuic, homoprotocatechuic, caffeic, α-resorcilic, p-hydroxyphenylacetic, p-hydroxybenzoic, p-coumaric, syringic, vanillic and ferulic. There are no substantial qualitative differences among the complex of phenolic acids in the investigated species. The RP-HPLC method was used for quanitative determination of phenolic acids. The amount of individual phenolic acids ranged between 2.2 µg/g and 147.8 µg/g in air-dry rhizome. The content of ferulic acid is the highest in all the examined cases. The total amount of the tested phenolic acids in <em>Rheum undulatum</em> L., <em>R. palmatum</em> L., and <em>R. raponticum</em> L. was respectively 346.4 µg/g, 229.8 µg/g, and 195 µg/g.

scholarly journals Cardiovascular Effects of Salvianolic Acid B

2013 ◽  
Vol 2013 ◽  
pp. 1-16 ◽  
Author(s):  
Jie Wang ◽  
Xingjiang Xiong ◽  
Bo Feng
Keyword(s):  
Phenolic Acids ◽  
Salvia Miltiorrhiza ◽  
Cardiovascular Effects ◽  
Underlying Mechanism ◽  
Salvianolic Acid B ◽  
Asian Countries ◽  
Biological Mechanisms ◽  
Clinical Therapy ◽  
Salvianolic Acid ◽  
Cardioprotective Effects

Salvianolic acid B (SAB, Sal B) is the representative component of phenolic acids derived from the dried root and rhizome ofSalvia miltiorrhizaBge (Labiatae) which has been used widely and successfully in Asian countries for clinical therapy of various vascular disturbance-related diseases for hundreds of years. However, its exact cardioprotective components and the underlying mechanism for therapeutic basis are still poorly understood. This paper discussed and elucidated the underlying biological mechanisms and pharmacology of Sal B and their potential cardioprotective effects.

Bioavailability of salvianolic acid B and effect on blood viscosities after oral administration of salvianolic acids in beagle dogs

2009 ◽  
Vol 32 (5) ◽  
pp. 773-779 ◽  
Author(s):  
Dong-Yan Gao ◽  
Li-Mei Han ◽  
Li-Hong Zhang ◽  
Xiao-Ling Fang ◽  
Jian-Xin Wang
Keyword(s):  
Oral Administration ◽  
Salvianolic Acid B ◽  
Beagle Dogs ◽  
Salvianolic Acid ◽  
Salvianolic Acids
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