Expression of antibodies using single-open reading frame vector design and polyprotein processing from mammalian cells

2009 ◽  
Vol 25 (3) ◽  
pp. 735-744 ◽  
Author(s):  
Yune Z. Kunes ◽  
Wendy R. Gion ◽  
Emma Fung ◽  
Jochen G. Salfeld ◽  
Rong-Rong Zhu ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Reading Frame ◽  
Polyprotein Processing

scholarly journals Expression of antibodies using single open reading frame (sORF) vector design

mAbs ◽  
2013 ◽  
Vol 5 (4) ◽  
pp. 595-607 ◽  
Author(s):  
Wendy R. Gion ◽  
Rachel A. Davis-Taber ◽  
Dean A. Regier ◽  
Emma Fung ◽  
Limary Medina ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Reading Frame

scholarly journals Spontaneous and induced mutations in a single open reading frame alter both virulence and avirulence in Xanthomonas campestris pv. vesicatoria avrBs2.

1996 ◽  
Vol 178 (15) ◽  
pp. 4661-4669 ◽  
Author(s):  
K M Swords ◽  
D Dahlbeck ◽  
B Kearney ◽  
M Roy ◽  
B J Staskawicz
Keyword(s):  
Xanthomonas Campestris ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Induced Mutations ◽  
Reading Frame

Evolutionarily conserved regions in Caenorhabditis transposable elements deduced by sequence comparison

Genome ◽  
1991 ◽  
Vol 34 (1) ◽  
pp. 6-12 ◽  
Author(s):  
Shiv S. Prasad ◽  
Linda J. Harris ◽  
David L. Baillie ◽  
Ann M. Rose
Keyword(s):  
Amino Acid ◽  
Transposable Element ◽  
Sequence Comparison ◽  
Horizontal Transmission ◽  
Protein A ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Major Open Reading Frame ◽  
Reading Frame ◽  
Caenorhabditis Species

In this paper we present the sequence of an intact Caenorhabditis briggsae transposable element, Tcb2. Tcb2 is 1606 base pairs in length and contains 80 base pair imperfect terminal repeats and a single open reading frame. We have identified blocks of T-rich repeats in the regions 150–200 and 1421–1476 of this element which are conserved in the Caenorhabditis elegans element Tc1. The sequence conservation of these regions in elements from different Caenorhabditis species suggests that they are of functional importance. A single open reading frame corresponding to the major open reading frame of Tc1 is conserved among Tc1, Tcb1, and Tcb2. Comparison of the first 550 nucleotides of the sequence among the three elements has allowed the evaluation of a model proposing an extension of the major open reading frame. Our data support the suggestion that Tc1 is capable of producing a 335 amino acid protein. A comparison of the sequence coding for the amino and carboxy termini of the 273 amino acid transposase from Caenorhabditis Tc1-like elements and Drosophila HB1 showed different amounts of divergence for each of these regions, indicating that the two functional domains have undergone different amounts of selection. Our data are not compatible with the proposal that Tc1-related sequences have been acquired via horizontal transmission. The divergence of Tc1 from the two C. briggsae elements, Tcb1 and Tcb2, indicated that all three elements have been diverging from each other for approximately the same amount of time as the genomes of the two species.Key words: Caenorhabditis, transposable element, sequence comparison.

scholarly journals Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame

Cell Research ◽  
2009 ◽  
Vol 19 (3) ◽  
pp. 296-306 ◽  
Author(s):  
Lijian Shao ◽  
Wei Feng ◽  
Yan Sun ◽  
Hao Bai ◽  
Jun Liu ◽  
...  
Keyword(s):  
Ips Cells ◽  
The Self ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Reading Frame

Expression studies of the HCV-1a core+1 open reading frame in mammalian cells

2008 ◽  
Vol 133 (2) ◽  
pp. 123-135 ◽  
Author(s):  
Niki Vassilaki ◽  
Haralabia Boleti ◽  
Penelope Mavromara
Keyword(s):  
Mammalian Cells ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Expression Studies

scholarly journals Tn917 transposase. Sequence correction reveals a single open reading frame corresponding to the tnpA determinant of Tn3-family elements

Plasmid ◽  
1991 ◽  
Vol 25 (2) ◽  
pp. 121-124 ◽  
Author(s):  
Florence Y. An ◽  
Don B. Clewell
Keyword(s):  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Reading Frame

scholarly journals Cryphonectria hypovirus 3, a Virus Species in the Family Hypoviridae with a Single Open Reading Frame

Virology ◽  
1999 ◽  
Vol 265 (1) ◽  
pp. 66-73 ◽  
Author(s):  
C.D. Smart ◽  
W. Yuan ◽  
R. Foglia ◽  
D.L. Nuss ◽  
D.W. Fulbright ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Virus Species ◽  
Reading Frame ◽  
The Family ◽  
Cryphonectria Hypovirus

scholarly journals RNA 2 of Citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand

2002 ◽  
Vol 83 (7) ◽  
pp. 1777-1781 ◽  
Author(s):  
María Eugenia Sánchez de la Torre ◽  
Carmelo López ◽  
Oscar Grau ◽  
María Laura García
Keyword(s):  
Blot Hybridization ◽  
Negative Polarity ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Northern Blot Hybridization ◽  
Complementary Strand ◽  
Significant Similarity ◽  
Reading Frame ◽  
A Genome ◽  
Citrus Psorosis Virus

Citrus psorosis virus (CPsV) causes a citrus disease occurring worldwide. Isolate CPV 4 has a genome with three single-stranded RNAs. The complete sequence of RNA 2 (1643 nucleotides) is reported here. Northern blot hybridization with strand-specific probes showed that most of the encapsidated RNA 2 is of negative polarity, although a small amount of the complementary strand may also be present in particles. The RNA 2 complementary strand contained a single open reading frame encoding a protein of 476 amino acids, which includes a motif resembling a nuclear localization signal. The sequence of this putative protein shows no significant similarity to any other in the databases. In the 3′-terminal untranslated region there is a putative polyadenylation signal. No subgenomic RNAs derived from RNA 2 were detected.

scholarly journals An Upstream Open Reading Frame and the Context of the Two AUG Codons Affect the Abundance of Mitochondrial and Nuclear RNase H1

2010 ◽  
Vol 30 (21) ◽  
pp. 5123-5134 ◽  
Author(s):  
Yutaka Suzuki ◽  
J. Bradley Holmes ◽  
Susana M. Cerritelli ◽  
Kiran Sakhuja ◽  
Michal Minczuk ◽  
...  
Keyword(s):  
Cell Death ◽  
Mitochondrial Dna ◽  
Translation Initiation ◽  
Mammalian Cells ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Tight Control ◽  
Reading Frame ◽  
Dual Localization ◽  
Control Of Expression

ABSTRACT RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a single mRNA. Here we show that expression levels of the two isoforms depend on the efficiency of translation initiation at each AUG codon and on the presence of a short upstream open reading frame (uORF) resulting in the mitochondrial isoform being about 10% as abundant as the nuclear form. Translation initiation at the M1 AUG is restricted by the uORF, while expression of the nuclear isoform requires reinitiation of ribosomes at the M27 AUG after termination of uORF translation or new initiation by ribosomes skipping the uORF and the M1 AUG. Such translational organization of RNase H1 allows tight control of expression of RNase H1 in mitochondria, where its excess or absence can lead to cell death, without affecting the expression of the nuclear RNase H1.

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