Evaluation of differences between dual salt-pH gradient elution and mono gradient elution using a thermodynamic model: Simultaneous separation of six monoclonal antibody charge and size variants on preparative-scale ion exchange chromatographic resin

2018 ◽  
Vol 34 (4) ◽  
pp. 973-986 ◽  
Author(s):  
Yi Feng Lee ◽  
Matthias Jöhnck ◽  
Christian Frech
Keyword(s):  
Monoclonal Antibody ◽  
Ion Exchange ◽  
Thermodynamic Model ◽  
Gradient Elution ◽  
Ph Gradient ◽  
Preparative Scale ◽  
Simultaneous Separation ◽  
Chromatographic Resin ◽  
Ph Gradient Elution

Modeling of salt and pH gradient elution in ion-exchange chromatography

2014 ◽  
Vol 37 (1-2) ◽  
pp. 5-13 ◽  
Author(s):  
Michael Schmidt ◽  
Mathias Hafner ◽  
Christian Frech
Keyword(s):  
Ion Exchange ◽  
Gradient Elution ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ph Gradient ◽  
Ph Gradient Elution

scholarly journals Stepwise pH-Gradient Elution for the Preparative Separation of Natural Anthraquinones by Multiple Liquid-Liquid Partition

2001 ◽  
Vol 56 (9-10) ◽  
pp. 719-725 ◽  
Author(s):  
Paavo H. Hynninen ◽  
Riikka Räisänen
Keyword(s):  
Aqueous Phase ◽  
Organic Phase ◽  
Concentration Profile ◽  
Gradient Elution ◽  
Solvent System ◽  
Ph Gradient ◽  
Preparative Scale ◽  
Ph Gradient Elution ◽  
Main Components ◽  
Partition Behavior

Abstract Preparative-scale separation of substituted anthraquinones by multiple liquid-liquid partition was studied using isopropylmethyl ketone (IM K)/aqueous phosphate buffer (aq.) as the solvent system and the Hietala apparatus with 100 partition units as the partition equipment. The lower (aq.) phase was chosen as mobile, while the upper (IMK) phase remained stationary. Hence, the principle of stepwise pH-gradient elution could be utilized to separate the components in two complex mixtures of hydroxyanthraquinones and hydroxyanthraquinone carboxylic acids, isolated from the fungus Dermocybe sanguinea. In spite of the nonlinearity of the partition isotherm s for these anthraquinones, implying deviations from the Nernst partition law, remarkable separations were achieved for the components in each mixture. Every anthraquinone carboxylic acid showed markedly irregular partition behavior, appearing in the effluent as two more or less resolved concentration zones. Such splitting was attributed to the formation of relatively stable sandwich-dimers, which were in a slow equilibrium with the monomers in the more nonpolar organic phase. At lower pH-values, only the polar monomers were distributed into the mobile aqueous phase and moved forward, whereas the nonpolar sandwich-dimers remained almost entirely in the stationary organic phase and lagged behind. When the pH of the mobile aqueous phase was raised high enough, the firmly linked dimers were monomerized and emerged from the apparatus as a second concentration profile. Intermolecular hydrogen bonding and π-π interaction between the n-systems of two anthraquinone molecules in a parallel orientation were considered responsible for the nonlinear and markedly irregular partition behavior of the natural anthraquinones studied. The nonlinearity of the partition behavior of the hydroxyanthraquinones lacking the carboxyl group, appeared merely as excessive broadening of the experimental concentration profile, which impaired the resolution between the components only insignificantly. Thus, e.g. the main components, dermocybin and emodin, could both be obtained from Separation 1 in a purity of at least 99% .

scholarly journals Improved enrichment strategies for phosphorylated peptides on titanium dioxide using methyl esterification and pH gradient elution

2008 ◽  
Vol 377 (2) ◽  
pp. 234-242 ◽  
Author(s):  
Eric S. Simon ◽  
Matthew Young ◽  
Antonia Chan ◽  
Zhao-Qin Bao ◽  
Philip C. Andrews
Keyword(s):  
Titanium Dioxide ◽  
Gradient Elution ◽  
Ph Gradient ◽  
Methyl Esterification ◽  
Phosphorylated Peptides ◽  
Ph Gradient Elution

Purification of camel liver catalase by zinc chelate affinity chromatography and pH gradient elution: An enzyme with interesting properties

2017 ◽  
Vol 1070 ◽  
pp. 104-111 ◽  
Author(s):  
Abdelbasset Chafik ◽  
Abdelkhalid Essamadi ◽  
Safinur Yildirim Çelik ◽  
Ahmet Mavi
Keyword(s):  
Affinity Chromatography ◽  
Gradient Elution ◽  
Ph Gradient ◽  
Ph Gradient Elution

Improving selectivity in multimodal chromatography using controlled pH gradient elution

2012 ◽  
Vol 1233 ◽  
pp. 152-155 ◽  
Author(s):  
Melissa A. Holstein ◽  
Amir A.M. Nikfetrat ◽  
Mark Gage ◽  
Allen G. Hirsh ◽  
Steven M. Cramer
Keyword(s):  
Gradient Elution ◽  
Ph Gradient ◽  
Multimodal Chromatography ◽  
Ph Gradient Elution
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