Ultrasound‐Assisted Extraction of Water‐Soluble Polysaccharides from the Fruit of Acanthopanax brachypus : Physicochemical, Structural and Functional Properties

Author(s):  
Haobin Hu ◽  
Haipeng Liang ◽  
Yufeng Wang ◽  
Runan Yuan ◽  
Jiao Sun ◽  
...  
2021 ◽  
pp. 2100092
Author(s):  
Fredy Morales‐Trejo ◽  
Daniel Trujillo‐Ramírez ◽  
Eleazar Aguirre‐Mandujano ◽  
Consuelo Lobato‐Calleros ◽  
E. Jaime Vernon‐Carter ◽  
...  

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 864 ◽  
Author(s):  
Yuan Fu ◽  
Shang Lin ◽  
Min Lu ◽  
Si-Yu Wei ◽  
Jia Zhou ◽  
...  

In the present study, an improved fluorometric assay based on aniline blue dye for the specific, accurate, and rapid quantification of 1,3-β-glucans in edible mushrooms was developed and fully validated. Furthermore, the improved method was successfully applied for the quantitative evaluation of water soluble 1,3-β-glucans extracted from Dictyophora indusiata by ultrasound-assisted extraction (UAE) with response surface methodology. Results showed that the improved method exhibited high specificity, accuracy, precision, repeatability, and stability, as well as a wide calibration range of 10–600 µg/mL (R2 > 99.9%). The maximum extraction yields of water soluble 1,3-β-glucans (1.20%) and total polysaccharides (5.41%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (56%), ultrasound extraction time (15 min), and ratio of liquid to raw material (22 mL/g). The results suggest that the improved fluorometric assay has great potential to be used as a routine method for the quantitative evaluation of 1,3-β-glucans in edible mushrooms and that the UAE method is effective for the extraction of 1,3-β-glucans from edible mushrooms.


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