scholarly journals Quantitative Evaluation of Ultrasound-Assisted Extraction of 1,3-β-glucans from Dictyophora indusiata Using an Improved Fluorometric Assay

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 864 ◽  
Author(s):  
Yuan Fu ◽  
Shang Lin ◽  
Min Lu ◽  
Si-Yu Wei ◽  
Jia Zhou ◽  
...  
Keyword(s):  
Quantitative Evaluation ◽  
Water Soluble ◽  
Fluorometric Assay ◽  
Edible Mushrooms ◽  
Raw Material ◽  
Blue Dye ◽  
Improved Method ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In the present study, an improved fluorometric assay based on aniline blue dye for the specific, accurate, and rapid quantification of 1,3-β-glucans in edible mushrooms was developed and fully validated. Furthermore, the improved method was successfully applied for the quantitative evaluation of water soluble 1,3-β-glucans extracted from Dictyophora indusiata by ultrasound-assisted extraction (UAE) with response surface methodology. Results showed that the improved method exhibited high specificity, accuracy, precision, repeatability, and stability, as well as a wide calibration range of 10–600 µg/mL (R2 > 99.9%). The maximum extraction yields of water soluble 1,3-β-glucans (1.20%) and total polysaccharides (5.41%) were achieved at the optimized extraction parameters as follows: ultrasound amplitude (56%), ultrasound extraction time (15 min), and ratio of liquid to raw material (22 mL/g). The results suggest that the improved fluorometric assay has great potential to be used as a routine method for the quantitative evaluation of 1,3-β-glucans in edible mushrooms and that the UAE method is effective for the extraction of 1,3-β-glucans from edible mushrooms.

scholarly journals Ultrasound-Assisted Extraction of Total Acetogenins from the Soursop Fruit by Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1139 ◽  
Author(s):  
Gabriela Aguilar-Hernández ◽  
María de los Ángeles Vivar-Vera ◽  
María de Lourdes García-Magaña ◽  
Napoleón González-Silva ◽  
Alejandro Pérez-Larios ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Large Scale ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Fruit Peel ◽  
Optimal Method ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
By Products

The soursop fruit or Annona muricata (A. muricata) fruit is recognized by its bioactive compounds and acetogenins (ACG) are among the most important. The effect of ACGs, with greater importance in health, is that they present anti-tumor activity; however, the methods of extraction of ACGs are very slow and with a high expenditure of solvents. To our knowledge, there is no report of an optimal method for the extraction of acetogenins from the Annonaceae family by ultrasound-assisted extraction (UAE); therefore, the aim was to find the best UEA conditions of acetogenins from A. muricata fruit (peel, pulp, seed, and columella) by using response surface methodology. The effect of amplitude (40%, 70%, and 100%), time (5, 10, and 15 min), and pulse-cycle (0.4, 0.7, and 1 s) of ultrasound at 24 kHz was evaluated on the total acetogenin content (TAC). Optimal extraction conditions of acetogenins (ACGs) with UEA were compared with the extraction of ACGs by maceration. The optimal UEA conditions in the A. muricata pulp and by-products were dependent on each raw material. The highest TAC was found in the seed (13.01 mg/g dry weight (DW)), followed by the peel (1.69 mg/g DW), the pulp (1.67 mg/g DW), and columella (1.52 mg/g DW). The experimental TAC correlated well with the model (Adjusted R2 with values between 0.88 and 0.97). The highest effectiveness in ACG extraction was obtained in seeds and peels using UEA compared to extraction by maceration (993% and 650%, respectively). The results showed that A. muricata by-products are an important source of ACGs and that UAE could be a viable alternative, with high potential for large-scale extraction.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Annona muricata By-Products and Pulp

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 904 ◽  
Author(s):  
Gabriela Aguilar-Hernández ◽  
María García-Magaña ◽  
María Vivar-Vera ◽  
Sonia Sáyago-Ayerdi ◽  
Jorge Sánchez-Burgos ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Raw Materials ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
By Products

Ultrasound-assisted extraction (UAE) is widely used; however, the efficiency of extraction depends on the raw materials. Therefore, optimization of UAE must be investigated for each type of plant material. By-products from soursop fruit have not been studied as a source of bioactive compounds. In this work, the optimization of UAE conditions (extraction time (5, 10, and 15 min), pulse cycle (0.4, 0.7, and 1 s), and sonication amplitude (40%, 70%, and 100%)) for the extraction of phenolic compounds (soluble, hydrolyzable, condensed tannins, and total polyphenols) from soursop by-products (seed, peel, and columella) and pulp was evaluated using response surface methodology. The optimal conditions for UAE to obtain the highest total polyphenol content from by-products and pulp was dependent on the raw material. Peel resulted in the highest content of total polyphenols (187.32 mg/g dry matter [DM]) followed by columella (164.14 mg/g DM), seed (36.15 mg/g DM), and pulp (33.24 mg/g DM). The yield of polyphenolic content from peel and columella obtained with UAE was higher (32–37%) than conventional extraction for 2 h under stirring (14–16%). The contents of gallic acid (0.36–15.86 µg/g DM), coumaric acid (0.07–1.37 µg/g DM), and chlorogenic acid (9.18–32.67 µg/g DM) in the different parts of the fruit were higher in the extracts obtained by UAE compared with a conventional extraction method (0.08–0.61, 0.05–0.08, 3.15–13.08 µg/g DM, respectively), although it was dependent on the raw materials. Soursop by-products can be functionally important if they are used to extract bioactive compounds by UAE; a technology with high potential for commercial extraction on a large scale.

scholarly journals Ultrasound-assisted extraction of water-soluble polysaccharides from the roots of valerian (Valeriana officinalis L.)

2002 ◽  
Vol 9 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Z. Hromádková ◽  
A. Ebringerová ◽  
P. Valachovič
Keyword(s):  
Water Soluble ◽  
Valeriana Officinalis ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Effect of temperature and time treatment of Ultrasound-assisted extraction on the properties of bengkoang water-soluble polysaccharides

2021 ◽  
Vol 332 ◽  
pp. 08004
Author(s):  
Adrian Hilman ◽  
Ismed Suhaidi ◽  
Azhari ◽  
Kridsada Keawyok
Keyword(s):  
Absorption Capacity ◽  
Water Soluble ◽  
Effect Of Temperature ◽  
Morphological Observation ◽  
Ultrasound Assisted Extraction ◽  
Time Treatment ◽  
Assisted Extraction ◽  
Soluble Polysaccharide ◽  
Ultrasound Assisted ◽  
Zero Hunger

Zero Hunger as the SDG program aims to prevent global need with innovation to exploit and promote local food uses of all available underutilized food crops. A water-soluble polysaccharide is included in bengkoang (Pachyrhizus erosus) soluble fibres. Previous studies reported that ultrasound-assisted extraction impacts starch depending on several factors such as temperatures and times. The research aims to obtain bengkoang oligosaccharide compounds by ultrasound-assisted extraction with different treatment of temperatures and times. The results of the moisture degree were decreasing the moisture degree might be attributed to the increasing temperature and time treatment of ultrasound-assisted extraction. It increases the water absorption capacity of the modified starch by sonication and is also related to the size of the starch granules. The results of the FTIR graph test show that some of the granules have cracks and small depressions on the surface that are visible in the middle and corners after being treated with sonication. The results of the morphological observation show that after treatment of sonication in water appear minor cracks and scratches. Some water-soluble polysaccharide granules are transformed into gelatinization form that makes granule shape irregular and has small visible cracks in the central part.

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