scholarly journals Structural basis of cyclic 1,3‐diene forming acyl‐coenzyme A dehydrogenases

ChemBioChem ◽  
2021 ◽  
Author(s):  
Johannes Kung ◽  
Anne-Katrin Meier ◽  
Max Willistein ◽  
Sina Weidenweber ◽  
Ulrike Demmer ◽  
...  
Keyword(s):  
Coenzyme A ◽  
Structural Basis ◽  
Acyl Coenzyme A

scholarly journals Structural basis for catalysis and substrate specificity of human ACAT1

2020 ◽  
Author(s):  
Hongwu Qian ◽  
Xin Zhao ◽  
Renhong Yan ◽  
Shuai Gao ◽  
Xue Sun ◽  
...  
Keyword(s):  
Catalytic Mechanism ◽  
Coenzyme A ◽  
Cholesterol Metabolism ◽  
Acyl Chain ◽  
Acyl Transfer ◽  
Structural Basis ◽  
Lateral Entry ◽  
Transmembrane Segments ◽  
Close Relationship ◽  
Acyl Coenzyme A

SummaryAcyl-coenzyme A: cholesterol acyltransferases (ACATs) catalyze acyl transfer from acyl-coenzyme A (CoA) to cholesterol to generate cholesteryl ester, which is the primary form for cellular storage and plasma transport of cholesterol. Because of their close relationship with cholesterol metabolism, ACATs represent potential drug target for the treatment of atherosclerosis and other cholesterol-related disorders. Here we present the cryo-EM structure of human ACAT1 at 3.3 Å resolution for dimer of dimers and 3.0 Å for a dimer. Each protomer consists of nine transmembrane segments that enclose a cytosolic (C) and a transmembrane (T) tunnel. The tunnels, each accommodating an elongated density, converge at the predicted catalytic site. Structure-guided mutational analyses suggest the cytosolic and lateral entry for acyl-CoA and cholesterol, respectively. Our structural, biochemical, and mass spectrometric characterizations reveal the catalytic mechanism and substrate preference for unsaturated acyl chain by ACAT1.

scholarly journals A Guanosine Triphosphate-dependent Acyl Coenzyme A Synthetase from Rat Liver Mitochondria

1967 ◽  
Vol 242 (9) ◽  
pp. 2111-2115 ◽  
Author(s):  
Lauro Galzigna ◽  
Carlo R. Rossi ◽  
Lodovico Sartorelli ◽  
David M. Gibson
Keyword(s):  
Rat Liver ◽  
Coenzyme A ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Guanosine Triphosphate ◽  
Acyl Coenzyme A
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