scholarly journals High Fidelity Enzyme‐Free Primer Extension with an Ethynylpyridone Thymidine Analog

Author(s):  
Clemens Richert ◽  
Jianyang Han ◽  
Eric Kervio
2018 ◽  
Vol 14 ◽  
pp. 603-617 ◽  
Author(s):  
Marilyne Sosson ◽  
Clemens Richert

The copying of short DNA or RNA sequences in the absence of enzymes is a fascinating reaction that has been studied in the context of prebiotic chemistry. It involves the incorporation of nucleotides at the terminus of a primer and is directed by base pairing. The reaction occurs in aqueous medium and leads to phosphodiester formation after attack of a nucleophilic group of the primer. Two aspects of this reaction will be discussed in this review. One is the activation of the phosphate that drives what is otherwise an endergonic reaction. The other is the improved mechanistic understanding of enzyme-free primer extension that has led to a quantitative kinetic model predicting the yield of the reaction over the time course of an assay. For a successful modeling of the reaction, the strength of the template effect, the inhibitory effect of spent monomers, and the rate constants of the chemical steps have to be determined experimentally. While challenges remain for the high fidelity copying of long stretches of DNA or RNA, the available data suggest that enzyme-free primer extension is a more powerful reaction than previously thought.


2018 ◽  
Vol 17 (3) ◽  
pp. 155-160 ◽  
Author(s):  
Daniel Dürr ◽  
Ute-Christine Klehe

Abstract. Faking has been a concern in selection research for many years. Many studies have examined faking in questionnaires while far less is known about faking in selection exercises with higher fidelity. This study applies the theory of planned behavior (TPB; Ajzen, 1991 ) to low- (interviews) and high-fidelity (role play, group discussion) exercises, testing whether the TPB predicts reported faking behavior. Data from a mock selection procedure suggests that candidates do report to fake in low- and high-fidelity exercises. Additionally, the TPB showed good predictive validity for faking in a low-fidelity exercise, yet not for faking in high-fidelity exercises.


2019 ◽  
Vol 12 (1) ◽  
pp. 18-33 ◽  
Author(s):  
Horea Pauna ◽  
Pierre-Majorique Léger ◽  
Sylvain Sénécal ◽  
Marc Fredette ◽  
Élise Labonté-Lemoyne ◽  
...  

1998 ◽  
Author(s):  
R. Hampton ◽  
Nagendra Subba Rao ◽  
Young Kim ◽  
William Wagar ◽  
Allen Karchmer

2021 ◽  
Author(s):  
Jian Xiao ◽  
Phillip Liu ◽  
D.C. Pham ◽  
Jim Lua ◽  
Shenal Perera ◽  
...  

2021 ◽  
Author(s):  
Mark Pankow ◽  
Joseph Giliberto ◽  
Brandon Hearley ◽  
Brian Justusson ◽  
Joseph Schaefer ◽  
...  

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