Use of a Novel Sub-2 µm Silica Hydride Vancomycin Stationary Phase in Nano-Liquid Chromatography. II. Separation of Derivatized Amino Acid Enantiomers

Chirality ◽  
2015 ◽  
Vol 27 (11) ◽  
pp. 767-772 ◽  
Author(s):  
Silvia Rocchi ◽  
Chiara Fanali ◽  
Salvatore Fanali
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Stationary Phase ◽  
Amino Acid Enantiomers ◽  
Silica Hydride ◽  
Nano Liquid Chromatography ◽  
Derivatized Amino Acid

Enantiomers separation by nano-liquid chromatography: Use of a novel sub-2μm vancomycin silica hydride stationary phase

2015 ◽  
Vol 1381 ◽  
pp. 149-159 ◽  
Author(s):  
Silvia Rocchi ◽  
Anna Rocco ◽  
Joseph J. Pesek ◽  
Maria T. Matyska ◽  
Donatella Capitani ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Silica Hydride ◽  
Nano Liquid Chromatography

Phospholipids covalently attached to silica particles as stationary phase in nano-liquid chromatography

2012 ◽  
Vol 71 ◽  
pp. 1-10 ◽  
Author(s):  
Clara-Eugenia Baños ◽  
Susanne K. Wiedmer ◽  
Jan-Henrik Smått ◽  
Motolani Sakeye ◽  
Jana Lokajová ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Silica Particles ◽  
Nano Liquid Chromatography

scholarly journals A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus

1984 ◽  
Vol 220 (1) ◽  
pp. 309-313 ◽  
Author(s):  
D L Hartley ◽  
M K Speedie
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Stationary Phase ◽  
High Pressure Liquid Chromatography ◽  
Absolute Configuration ◽  
Specific Activity ◽  
Shake Culture ◽  
Methyl Group ◽  
The Absolute

A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.

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