Direct HPLC separation of enantiomers of pantoprazole and other benzimidazole sulfoxides using cellulose-based chiral stationary phases in reversed-phase mode

Chirality ◽  
1995 ◽  
Vol 7 (8) ◽  
pp. 612-615 ◽  
Author(s):  
Makoto Tanaka ◽  
Hideki Yamazaki ◽  
Hideo Hakusui
Keyword(s):  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Hplc Separation ◽  
Separation Of Enantiomers

Chromatographic separation of enantiomers on network—polymeric chiral stationary phases

1996 ◽  
Vol 741 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Shalini Andersson ◽  
Stig Allenmark ◽  
Per Möller ◽  
Börje Persson ◽  
Domingo Sanchez
Keyword(s):  
Chromatographic Separation ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Separation Of Enantiomers ◽  
Polymeric Chiral Stationary Phases

scholarly journals Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

2021 ◽  
Vol 14 (1) ◽  
pp. 60-65
Author(s):  
Laura Jánovová ◽  
Katarína Hroboňová
Keyword(s):  
Lactic Acid ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Optical Isomers ◽  
Column Temperature ◽  
Retention Factors ◽  
Chromatographic Parameters ◽  
Macrocyclic Antibiotics

Abstract Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (R S = 1.9 ) and ristocetin (R S = 1.7 ) in reversed-phase separation mode at the column temperature of 25 °C.

scholarly journals The Separation of Cannabinoids on Sub-2 µm Immobilized Polysaccharide Chiral Stationary Phases

2021 ◽  
Vol 14 (12) ◽  
pp. 1250
Author(s):  
Takafumi Onishi ◽  
Weston J. Umstead
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Elution Order ◽  
Testing Methods ◽  
Structural Isomers

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

Sign in / Sign up

Export Citation Format

Share Document