A high molecular weight centrosomal protein of mammalian cells is antigenically related to myosin II

1992 ◽  
Vol 23 (2) ◽  
pp. 122-132 ◽  
Author(s):  
Eric Bailly ◽  
Nicoles Bordes ◽  
Michel Bornens ◽  
Catherine Klotz
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Myosin Ii ◽  
Centrosomal Protein

scholarly journals Dymple, a Novel Dynamin-like High Molecular Weight GTPase Lacking a Proline-rich Carboxyl-terminal Domain in Mammalian Cells

1998 ◽  
Vol 273 (2) ◽  
pp. 1044-1051 ◽  
Author(s):  
Takahiro Kamimoto ◽  
Yasuo Nagai ◽  
Hiroshi Onogi ◽  
Yoshinao Muro ◽  
Takashi Wakabayashi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Terminal Domain ◽  
Carboxyl Terminal Domain ◽  
Carboxyl Terminal

Association of c-myc protein with enzymes of DNA replication in high molecular weight fractions from mammalian cells

1991 ◽  
Vol 147 (3) ◽  
pp. 412-419 ◽  
Author(s):  
George P. Studzinski ◽  
Uma T. Shankavaram ◽  
Dorothy C. Moore ◽  
Prem Veer Reddy
Keyword(s):  
Molecular Weight ◽  
Dna Replication ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Molecular Weight Fractions

Synthese und Umsatz von Ribonukleinsäuren in suspendierten Säugetierzellen / Synthesis and Turnover of Ribonucleic Acids in Suspended Mammalian Cells

1972 ◽  
Vol 27 (6) ◽  
pp. 714-717
Author(s):  
Günther Trams ◽  
Ingeborg Vollertsen ◽  
Gebhard Koch
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Nucleotide Composition ◽  
X Rays ◽  
Ribonucleic Acids ◽  
Salt Gradient ◽  
Precursor Rna ◽  
Labeled Rna ◽  
Ribosomal Precursor

The influence of ionizing radiation on the turnover of high molecular weight RNA in mammalian cells was studied. Growing cultures of suspended human amnion cells were labeled with 32P and irradiated with different doses of X-rays. RNA was isolated by hot phenol and the high molecular weight RNA fractionated by chromatography on methylated serum albumin celite columns. The salt gradient eluted RNA with sedimentation coefficients of 16 S or greater, which were characterized by analysis of nucleotide composition.Irradiation with doses from 900—6000 R results in degradation of pre-labeled RNA. A quantitative determination reveals that more 32P-radioactivity leaves the high molecular weight RNA compared to labeled total material that is degraded to acid soluble products. This indicates that RNA is partly broken down to low molecular weight fragments.Nucleotide analysis of the high molecular weight RNA fractions indicate a preferred degradation of ribosomal precursor RNA.The experimental results give evidence that the analysis of RNA synthesis for a certain period after irradiation is complicated by interfering processes like the simultaneous degradation of RNA.

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