Nerve growth factor (NGF) receptor expression in chicken cranial development

beta-Nerve growth factor (NGF) is expressed in spermatogenic cells and has testosterone-downregulated low-affinity receptors on Sertoli cells suggesting a paracrine role in the regulation of spermatogenesis. An analysis of the stage-specific expression of NGF and its low affinity receptor during the cycle of the seminiferous epithelium in the rat revealed NGF mRNA and protein at all stages of the cycle. Tyrosine kinase receptor (trk) mRNA encoding an essential component of the high-affinity NGF receptor was also present at all stages. In contrast, expression of low affinity NGF receptor mRNA was only found in stages VIIcd and VIII of the cycle, the sites of onset of meiosis. The low-affinity NGF receptor protein was present in the plasma membrane of the apical Sertoli cell processes as well as in the basal plasma membrane of these cells at stages VIIcd to XI. NGF was shown to stimulate in vitro DNA synthesis of seminiferous tubule segments with preleptotene spermatocytes at the onset of meiosis while other segments remained nonresponsive. We conclude that NGF is a meiotic growth factor that acts through Sertoli cells.

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CRK protein binds to two guanine nucleotide-releasing proteins for the Ras family and modulates nerve growth factor-induced activation of Ras in PC12 cells

Molecular and Cellular Biology ◽

10.1128/mcb.14.8.5495-5500.1994 ◽

1994 ◽

Vol 14 (8) ◽

pp. 5495-5500

Author(s):

M Matsuda ◽

Y Hashimoto ◽

K Muroya ◽

H Hasegawa ◽

T Kurata ◽

...

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Pc12 Cells ◽

Sh2 Domain ◽

Single Amino Acid ◽

Guanine Nucleotide ◽

Nerve Growth ◽

Tyrosine Residues ◽

Ngf Receptor ◽

Ras Family

It has been reported that growth factors activate Ras through a complex of an adaptor type SH2-containing molecule, Grb2, and a Ras guanine nucleotide-releasing protein (GNRP), mSos. We report on the involvement of another adaptor molecule, CRK, in the activation of Ras. Overexpression of wild-type CRK proteins CRK-I and CRK-II enhanced the nerve growth factor (NGF)-induced activation of Ras in PC12 cells, although the basal level of GTP-bound active Ras was not altered. In contrast, mutants with a single amino acid substitution in either the SH2 or SH3 domain of the CRK-I protein inhibited the NGF-induced activation of Ras. Two GNRPs for the Ras family, mSos and C3G, were coimmunoprecipitated with the endogenous Crk proteins in PC12 cells. The association between C3G and the CRK mutants was dependent upon the presence of intact SH3. The SH2 domain of CRK bound to the SHC protein phosphorylated on tyrosine residues by NGF stimulation. The results demonstrate that, in addition to Grb2, CRK participates in signaling from the NGF receptor and that two GNRPs appear to transmit signals from these adaptor molecules to Ras.

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Immunohistochemical analysis of nerve growth factor receptor expression in normal and malignant human tissues.

Journal of Histochemistry & Cytochemistry ◽

10.1177/36.4.2831267 ◽

1988 ◽

Vol 36 (4) ◽

pp. 383-389 ◽

Cited By ~ 91

Author(s):

P G Chesa ◽

W J Rettig ◽

T M Thomson ◽

L J Old ◽

M R Melamed

Keyword(s):

Nervous System ◽

Nerve Growth Factor ◽

Growth Factor ◽

Normal Development ◽

Malignant Tumors ◽

Sympathetic Neurons ◽

Growth Factor Receptor ◽

Nerve Growth Factor Receptor ◽

Receptor Expression ◽

Nerve Growth

Nerve growth factor (NGF) is a polypeptide important for normal development of the nervous system and promotion of survival and differentiation of sensory and sympathetic neurons in culture. The cellular effects of NGF are mediated by a specific cell surface molecule, nerve growth factor receptor (NGF-R). In the present study we have used a monoclonal antibody against human NGF-R to examine, by the avidin-biotin-immunoperoxidase method, the receptor distribution in a wide range of normal tissues and in more than 200 malignant tumors. Our results show that (a) human NGF-R is expressed in the peripheral nervous system but not in any of the central nervous system areas tested; (b) NGF-R expression is not restricted to neural tissues but is also found in a number of normal epithelial, mesenchymal, and lymphoid tissues; (c) NGF-R expression changes during normal development; and (d) NGF-R expression in malignant tumors generally parallels its normal tissue distribution. Thus, NGF-R is detected in a proportion of neuroectoderm-derived tumors, carcinomas, and lymphomas, and also in a characteristic group of small round-cell tumors (Ewing's sarcomas and embryonal rhabdomyosarcomas). These findings suggest a normal regulatory role for NGF in both neuronal and non-neuronal cells and identify a range of human tumors in which the NGF/NGF-R system may contribute to the malignant phenotype.

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Nerve growth factor receptor expression in lymphocytes: Comment on the article by Raychaudhuri et al

Arthritis & Rheumatism ◽

10.1002/art.34366 ◽

2012 ◽

Vol 64 (4) ◽

pp. 1295-1296

Author(s):

Natacha Ralainirina ◽

François Hentges ◽

Jacques Zimmer

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Growth Factor Receptor ◽

Nerve Growth Factor Receptor ◽

Receptor Expression ◽

Nerve Growth

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Human Nerve Growth Factor Receptor Expression in the CNS of Transgenic Mice

Growth Factors and Alzheimer’s Disease ◽

10.1007/978-3-642-46722-6_6 ◽

1991 ◽

pp. 62-72

Author(s):

N. Patil ◽

E. Lacy ◽

M. V. Chao

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Transgenic Mice ◽

Growth Factor Receptor ◽

Nerve Growth Factor Receptor ◽

Receptor Expression ◽

Nerve Growth ◽

Human Nerve

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Expression of nerve growth factor and neurotrophin receptors in testicular cells suggest novel roles for neurotrophins outside the nervous system

Reproduction Fertility and Development ◽

10.1071/rd9961075 ◽

1996 ◽

Vol 8 (7) ◽

pp. 1075 ◽

Cited By ~ 24

Author(s):

K Seidl ◽

A Buchberger ◽

C Erck

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Germ Cells ◽

Sertoli Cells ◽

Leydig Cells ◽

Expression Patterns ◽

Myoid Cells ◽

Nerve Growth ◽

High Affinity ◽

Ngf Receptor

The present study was designed to clarify the non-neurotrophic role for neurotrophins in mouse testis. By means of SI nuclease protection assay we could demonstrate that the gene coding for the low-affinity nerve growth factor (NGF) receptor p75NGFR is transiently expressed during germ cell development. Gene expression for p75NGFR was detected in late-meiotic spermatocytes and early spermatids and was found to be co-expressed with trkB and trkC, two tyrosine kinase receptors, commonly regarded as the high-affinity receptors for brain-derived neurotrophic factor and neurotrophin-3. Gene transcripts for the high-affinity NGF receptor trkA were found exclusively in non-germ cells. Isolated Leydig cells, peritubular myoid cells and Sertoli cells, but not germ cells, could be identified as potential testicular NGF sources. Non-germ cells respond after incubation for several days with a sharp induction in NGF synthesis, which is accompanied by a loss of phenotypic expression patterns. The fact that p75NGFR mRNA expression was induced in cultured Sertoli cells and peritubular myoid cells suggests an autocrine mode of NGF action in these cells. Induction of NGF synthesis in cultured Leydig cells could be prevented by the glucocorticoid dexamethasone. Results indicate different roles for the individual neurotrophins in distinct testicular compartments and suggest that these neurotrophins might support testicular functions by signalling between individual cell types in an autocrine and paracrine manner.

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