The dynamics of a healthy and infected red blood cell in flow through constricted channels: A DPD simulation

In the blood flow through microvessels, platelets show high concentrations near the vessel wall. This phenomenon is called margination of platelets and is closely associated with hemostasis and thrombosis. In the present study, we conducted in vitro experiments using platelet-sized fluorescent particles as platelet substitutes to investigate the cross-sectional distribution of these particles in the red blood cell suspension flowing through microchannels with a square cross section. Fluorescence observations were performed to measure the transverse distribution of particles at various heights from the bottom face with the use of a confocal laser scanning microscope system. In downstream cross sections of the channel, particles showed focusing near the four corners rather than uniform margination along the entire circumference of the cross section. The focusing of particles near the corners was more enhanced for higher hematocrits. On the other hand, particles in circular channel flows showed nearly axisymmetric uniform accumulation adjacent to the channel wall. The present result suggests that the segregation of suspended particles in the flow of multicomponent suspensions could have such heterogeneous 2D features of particle distribution in the cross section of channels, especially for rectangular channels often used in microfluidics.

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Indirect antiglobulin paper test for red blood cell antigen typing by flow-through method

Analytical Methods ◽

10.1039/c5ay00744e ◽

2015 ◽

Vol 7 (11) ◽

pp. 4645-4649 ◽

Cited By ~ 7

Author(s):

Natasha Yeow ◽

Heather McLiesh ◽

Gil Garnier

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Paper Test ◽

Cell Antigen ◽

Flow Through ◽

Simple Flow

Simple flow-through based indirect antiglobulin paper test.

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A compartmental analysis of the splenic circulation in rat

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1983.245.1.h17 ◽

1983 ◽

Vol 245 (1) ◽

pp. H17-H21 ◽

Cited By ~ 3

Author(s):

R. J. Stock ◽

E. V. Cilento ◽

F. D. Reilly ◽

R. S. McCuskey

Keyword(s):

Bovine Serum Albumin ◽

Blood Cell ◽

Plasma Flow ◽

Red Blood Cell ◽

Compartmental Analysis ◽

Metabolic Demand ◽

Plasma Skimming ◽

Flow Through ◽

Filtration Capacity ◽

Red Pulp

Isolated, acellular washout experiments of 125I-labeled bovine serum albumin (BSA) from control, anemic, and polycythemic rat spleens were used to develop a model of the splenic plasma circulation. The results indicated that the plasma circulation can be described adequately by two compartments. As in red blood cell (RBC) washouts [Am. J. Physiol. 239 (Heart Circ. Physiol. 8): H272-H277, 1980] the fast compartment represents intrasplenic vessels that bypass the red pulp, whereas the intermediate/slow compartment represents plasma flow through the red pulp (filter). The combined plasma and RBC parameters suggest the rat spleen is not an RBC reservoir and that splenic RBC filtration capacity decreases during polycythemia and anemia. The ratio of fast compartment to systemic hematocrit indicates hemodilution occurs, supporting the concept of plasma skimming. A small plasma holdup occurs in the red pulp of anemic and polycythemic spleens probably due to RBC congestion. This congestion, in turn, might be due to reticulocyte sequestration and/or erythropoiesis in anemic spleens and RBC sequestration and/or destruction in polycythemic spleens. There is plasma redistribution in polycythemic spleens possibly to meet the increased metabolic demand.

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