scholarly journals Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity

2020 ◽  
Vol 131 (1) ◽  
Author(s):  
Pawin Pongkorpsakol ◽  
Jerrold R. Turner ◽  
Li Zuo
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Size Selectivity ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Vitro Analysis ◽  
Macromolecular Permeability ◽  
In Vitro Analysis

Acanthamoeba culbertsoni isolated from a clinical case with intraocular dissemination: Structure and in vitro analysis of the interaction with hamster cornea and MDCK epithelial cell monolayers

2017 ◽  
Vol 183 ◽  
pp. 245-253 ◽  
Author(s):  
Arturo González-Robles ◽  
Maritza Omaña-Molina ◽  
Lizbeth Salazar-Villatoro ◽  
Catalina Flores-Maldonado ◽  
Jacob Lorenzo-Morales ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Clinical Case ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Acanthamoeba Culbertsoni ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Dysregulation of junctional adhesion molecule-A contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers

2017 ◽  
Vol 5 (23) ◽  
pp. e13541 ◽  
Author(s):  
Daniel M. Chopyk ◽  
Pradeep Kumar ◽  
Reben Raeman ◽  
Yunshan Liu ◽  
Tekla Smith ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Barrier Disruption ◽  
Epithelial Cell Monolayers

Oxygen free radical injury of IEC-18 small intestinal epithelial cell monolayers

1991 ◽  
Vol 100 (6) ◽  
pp. 1533-1543 ◽  
Author(s):  
Thomas Y. Ma ◽  
Daniel Hollander ◽  
Doug Freeman ◽  
Thang Nguyen ◽  
Pavel Krugliak
Keyword(s):  
Free Radical ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Oxygen Free Radical ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Small Intestinal Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Small Intestinal

PSIII-21 Efficacy of deoxynivalenol detoxification by sodium metabisulfite (SMBS) containing nanofibrous mats using an in vitro intestinal epithelial cell IPEC-J2

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 341-342
Author(s):  
Changning Yu ◽  
Quintin Litke ◽  
Peng Lu ◽  
Shangxi Liu ◽  
Joshua Gong ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Sodium Metabisulfite ◽  
Intestinal Epithelial ◽  
Cell Integrity ◽  
Eudragit L100 ◽  
Vitro Analysis

Abstract Deoxynivalenol (DON) occurs in many commonly used cereal grains. Pigs feed with DON concentrations as low as 0.6–2.0 mg/kg can result in reduced feed intake and growth rate, damage to intestinal epithelial cells, and increase susceptibility to enteric pathogens. Sodium metabisulfite (SMBS) can efficiently detoxify DON by converting it 8-DONS or 10-DONS in vitro. However, if SMBS is added directly to the feed, SMBS rapidly degrades under acidic aqueous conditions (e.g. pig stomach) and little SMBS is delivered to the intestinal absorption site where it can effectively detoxify DON. Thus, the objective of this study was to encapsulate SMBS into Eudragit L100-55 nanofibrous mats to deliver intact SMBS to the small intestine and evaluate the efficacy of DON detoxification in the simulated intestine fluid (SIF) using an in vitro intestinal epithelial cell (IPEC-J2) model. Nanofibrous mats were produced by coaxial electrospinning, with peak loading capacity and loading efficiency of SMBS reaching 32.00% and 80.01%. DON-induced cytotoxicity was not observed during in vitro analysis consisting of incubation of DON in the presence of SMBS-containing nanofibers (0.5% w/w) in simulated gastric fluid (SGF) for 2 h followed by incubation in a mixture of SGF and SIF (1:1) for 20 min. Meanwhile, compared to the DON treatment, incubation of DON in the presence of SMBS-containing nanofiber (0.5% w/w) in SGF for 2 h and SIF for 20 min decreased the gene expression of inflammatory cytokines in the IPEC-J2 cells and maintained the cell integrity. To conclude, SMBS released from Eudragit L100-55 nanofibrous mats in the SIF effectively decreased the adverse effects induced by DON in the IPEC-J2 cells. Nanofibrous mats can release a large amount of SMBS in a short time in SIF to achieve the effect of detoxifying DON.

scholarly journals Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Emily C. Moorefield ◽  
R. Eric Blue ◽  
Nancy L. Quinney ◽  
Martina Gentzsch ◽  
Shengli Ding
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Functional Analyses

Polarized transport of docetaxel and vinblastine mediated by P-glycoprotein in human intestinal epithelial cell monolayers

1994 ◽  
Vol 48 (7) ◽  
pp. 1528-1530 ◽  
Author(s):  
Pierre Wils ◽  
Véronique Phung-Ba ◽  
Armelle Warnery ◽  
Delphine Lechardeur ◽  
Shamsi Raeissi ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cell ◽  
Cell Monolayers ◽  
P Glycoprotein ◽  
Epithelial Cell Monolayers ◽  
Polarized Transport

scholarly journals Generation of Human iPSC–Derived Intestinal Epithelial Cell Monolayers by CDX2 Transduction

2019 ◽  
Vol 8 (3) ◽  
pp. 513-526 ◽  
Author(s):  
Kazuo Takayama ◽  
Ryosuke Negoro ◽  
Tomoki Yamashita ◽  
Kanae Kawai ◽  
Moe Ichikawa ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Human Ipsc
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