scholarly journals Laboratory Maintenance and Growth of Talaromyces marneffei

2020 ◽  
Vol 56 (1) ◽  
Author(s):  
Alex Andrianopoulos
Keyword(s):  
Talaromyces Marneffei ◽  
Laboratory Maintenance

scholarly journals The novel Dbl homology/BAR domain protein, MsgA, of Talaromyces marneffei regulates yeast morphogenesis during growth inside host cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Harshini Weerasinghe ◽  
Hayley E. Bugeja ◽  
Alex Andrianopoulos
Keyword(s):  
Pathogenic Fungi ◽  
Host Cells ◽  
Microbial Pathogens ◽  
Mammalian Host ◽  
Host Immune System ◽  
Nucleotide Exchange ◽  
Phagocytic Cells ◽  
Macrophage Infection ◽  
Bar Domain ◽  
Talaromyces Marneffei

AbstractMicrobial pathogens have evolved many strategies to evade recognition by the host immune system, including the use of phagocytic cells as a niche within which to proliferate. Dimorphic pathogenic fungi employ an induced morphogenetic transition, switching from multicellular hyphae to unicellular yeast that are more compatible with intracellular growth. A switch to mammalian host body temperature (37 °C) is a key trigger for the dimorphic switch. This study describes a novel gene, msgA, from the dimorphic fungal pathogen Talaromyces marneffei that controls cell morphology in response to host cues rather than temperature. The msgA gene is upregulated during murine macrophage infection, and deletion results in aberrant yeast morphology solely during growth inside macrophages. MsgA contains a Dbl homology domain, and a Bin, Amphiphysin, Rvs (BAR) domain instead of a Plekstrin homology domain typically associated with guanine nucleotide exchange factors (GEFs). The BAR domain is crucial in maintaining yeast morphology and cellular localisation during infection. The data suggests that MsgA does not act as a canonical GEF during macrophage infection and identifies a temperature independent pathway in T. marneffei that controls intracellular yeast morphogenesis.

A norbisabolane and an arabitol benzoate from Talaromyces marneffei, an endophytic fungus of Epilobium angustifolium

Fitoterapia ◽  
2021 ◽  
pp. 104948
Author(s):  
Zhong-Duo Yang ◽  
Xu-Dong Zhang ◽  
Xing Yang ◽  
Xiao-Jun Yao ◽  
Zong-Mei Shu
Keyword(s):  
Endophytic Fungus ◽  
Talaromyces Marneffei ◽  
Epilobium Angustifolium

scholarly journals An investigation into the possible regulation of the expression of genes by yapA in Talaromyces marneffei using the qRT- PCR method

2017 ◽  
Vol 56 (6) ◽  
pp. 735-745 ◽  
Author(s):  
Wiyada Dankai ◽  
Monsicha Pongpom ◽  
Nongnuch Vanittanakom
Keyword(s):  
Talaromyces Marneffei ◽  
Qrt Pcr ◽  
Expression Of Genes ◽  
Pcr Method

scholarly journals HIV-negative case of Talaromyces marneffei pulmonary infection with a TSC2 mutation

2021 ◽  
Vol 49 (5) ◽  
pp. 030006052110167
Author(s):  
Qian Shen ◽  
Lingyan Sheng ◽  
Jianying Zhou
Keyword(s):  
Pulmonary Infection ◽  
Pathogenic Fungus ◽  
Critical Role ◽  
Lavage Fluid ◽  
Tsc2 Gene ◽  
Current Case ◽  
Negative Patient ◽  
Talaromyces Marneffei ◽  
Negative Case ◽  
Hiv Negative

Talaromyces marneffei is a rare dimorphic pathogenic fungus that can induce severe infections in human immunodeficiency virus (HIV)-infected patients. However, such infections have also been reported in non-HIV hosts. This current case report describes a very rare case of a T. marneffei pulmonary infection in an HIV-negative patient with a mutation in the tuberous sclerosis complex subunit 2 ( TSC2) gene. A 24-year-old male patient presented with cough and expectoration for 6 months. Computed tomography showed multiple ground-glass opacities and cystic and cavitated lesions in both lungs. Next generation sequencing (NGS) of the bronchoalveolar lavage fluid was performed to confirm T. marneffei pulmonary infection. The results were further verified using bronchoscopy specimen cultures. This was an HIV-negative patient without a travel history to endemic zones and his blood exon sequencing results showed a mutation in the TSC2 gene. To date, he has recovered well with voriconazole therapy. In summary, patients with TSC2 mutations that induce bronchopulmonary dysplasia may be potential hosts for T. marneffei. Early and timely diagnosis is important for improving prognosis. NGS plays a critical role in the diagnosis of T. marneffei pulmonary infection.

Laboratory Maintenance of Phlebotomine Sand Flies

2014 ◽  
pp. 131-164
Author(s):  
Michelle Colacicco-Mayhugh
Keyword(s):  
Sand Flies ◽  
Phlebotomine Sand Flies ◽  
Laboratory Maintenance

Techniques and Laboratory Maintenance of Cryptosporidium

2018 ◽  
pp. 31-50 ◽  
Author(s):  
W. L. Current
Keyword(s):  
Laboratory Maintenance

Preservation of diversity and oenological properties of wine yeasts during long-term laboratory maintenance: A study of strains of a century-old Tokaj wine yeast collection

LWT ◽  
2019 ◽  
Vol 101 ◽  
pp. 789-798 ◽  
Author(s):  
Zoltán Kállai ◽  
Walter P. Pfliegler ◽  
Judit Mitercsák ◽  
Gergő Szendei ◽  
Matthias Sipiczki
Keyword(s):  
Wine Yeast ◽  
Wine Yeasts ◽  
Laboratory Maintenance
Sign in / Sign up

Export Citation Format

Share Document