Application Design of Web-based Computer Laboratory Inventory and Maintenance System at SMK Bhakti Anindya

This paper describes a problem in computer laboratory maintenance and inventory. Inventory of goods in Every company or institution always has a connection with the data management process of goods / equipment owned by the company. In addition to inventory, maintenance is also very necessary in order to keep company assets under control, safe, and always maintained. For this reason, an Inventory Information System is needed as well as a maintenance system in managing the data. The results of this design are focused on managing information about the management of goods and several aspects regarding the maintenance of goods, especially in the computer laboratory of SMK Bhakti Anindya. With the implementation of the maintenance information system and laboratory inventory at Bhakti Anindya Vocational School, data management and equipment maintenance will be better. Tulisan ini menjelaskan mengenai sebuah permasalahan dalam perawatan dan inventaris laboratorium komputer. Inventarisasi barang dalam Setiap perusahaan atau sebuah lembaga selalu memiliki keterkaitan dengan proses pengelolaan data barang / peralatan yang di miliki oleh perusahaan tersebut. Disamping inventarisasi, hal perawatan juga sangat diperlukan demi menjaga agar aset perusahaan terkontrol, aman, dan selalu terawat. Untuk itulah di butuhkan suatu Sistem Informasi Inventaris sekaligus sistem perawatannya dalam mengelola data tersebut. Hasil dari rancang bangun ini dititik beratkan pada pengelolaan informasi tentang pengelolaan barang dan beberapa aspek mengenai perawatan barang terutama pada bagian laboratorium komputer SMK Bhakti Anindya. Dengan diimplementasikannya sistem informasi Perawatan dan inventaris laboratorium di SMK Bhakti Anindya, pengelolaan data barang dan perawatan alat akan lebih baik.

Prolonged Laboratory Maintenance of Toxoplasma gondii Tachyzoites in Co-cultivation with the Bovine Theileria annulata-Infected Lymphoblastoids

Background and Aims: In most of the studies, Toxoplasma gondii is maintained in laboratory mice or studied in vitro using non-lymphoid cell lines or primary mouse macrophages. The target of our research was to design a new axenic culture of Toxoplasma gondii tachyzoites to providing a sufficient quantity of them. Material and Methods: Theileria annulata-infected lymphoblastoids, which had been maintained up to 260 sub-cultures to attenuate the Theileria annulata, were evaluated for their suitability to the cultivation of Toxoplasma gondii tachyzoites. This cultivation process was carried out continuously for up to 10 passages, and after each 5 sub-culture, 0.1 ml of culture suspension (1×106 tachyzoites) was inoculated to each BALB/c mouse. Results: It was observed that the tachyzoites have attacked the lympho blastoids, multiplied inside them, and many fresh tachyzoites with typical shape and gliding movement were present in the culture suspension. In all processes of cultivation, the pathogenesis of parasites remained stable, and they were able to proliferate in mice and eventually lead to the death of the animals. Conclusions: We describe here a new protocol for prolonged maintenance of tachyzoites of Toxoplasma gondii, which is more efficient (both in terms of yield and cost (it does not need fetal calf serum)) than other traditional methods for maintenance of the parasite.

Selection of non-target Lepidoptera species to test Bt maize effects in the laboratory: which species and how to breed them?

Bt maize targeting Lepidopteran pests poses potential risks for non-target (NT) butterflies and moths which are addressed in the environmental risk assessment of genetically modified crop plants. For this purpose, eco-toxicological tests are often conducted with specific NT species in the laboratory in order to assess possible adverse effects. As only a limited number of surrogate species can be addressed, the choice of focal species to be tested is an important decision. However, practical and standardised selection procedures have hardly been developed and applied for NTLepidoptera, so far. Here, we present a transparent and systematic selection process of suitable test species for Germany, involving selection criteria such as exposure to Bt maize, habitat range and laboratory maintenance of the species. As a result, we compiled a list of 15 lepidopteran species particularly appropriate for testing the adverse effects of Bt maize in the laboratory. In addition, we collected and reviewed published reports for breeding methods of Lepidoptera, which provides essential information on maintaining lab stocks of NTLepidoptera. The presented selection procedure allows focusing on the relevant test species in a transparent and reproducible way, and supplies the breeding knowledge required to breed and maintain them, which will be of great utility for the future assessment on possible risks of Bt maize cultivation to non-target Lepidoptera.

Investigation of flagellates in sandfly guts by microscopic observation

Background The identification of natural Leishmania infection, observing the location of flagellates in the gut and development stages of promastigotes, helps to incriminate a certain species as vector and to assess the infection risk in host populations, thus contributing with leishmaniasis surveillance. Objectives To document with photos, videos and description of the dissection process of sandfly females for observation in their digestive tract of Leishmania infections of Leishmania and Viannia subgenera (suprapillary and peripillary distribution, respectively), to produce a manual to assist in leishmaniasis surveillance actions. Methods For the documentation of suprapillary infection, Lutzomyia longipalpis females fed on hamsters infected with Leishmania (L.) infantum and for peripillary infection, Pintomyia fischeri and Nyssomyia neivai fed on hamsters infected with L (V.) braziliensis were used. The dissection of the females was performed at intervals of 12 hours after infectious repast, until completing 120 hours, to observe the different phases of the parasite's evolutionary cycle in the gut. Results A manual was produced with description and photos of the entire process, from field sandfly collection, transportation, laboratory maintenance and dissection, as well as the complete cycle of the parasite's evolution inside the vector. Videos were also produce. Conclusions This project sought to contribute with the leishmaniasis surveillance as regards the sandfly natural infection investigation by Leishmania to assess the transmission risk of parasites. Although the identification of the Leishmania species depends on molecular techniques, this initial screening may reduce its costs. Key messages This study enabled the elaboration of a support manual for technicians from the Entomology laboratory networks. This study enabled with information to identify infected sandflies and thus colaborate with the leishmaniasis epidemiological surveillance programs.