Establishment of a Human Blood-Brain Barrier Co-Culture Model Mimicking the Neurovascular Unit Using Induced Pluripotent Stem Cells

2018 ◽  
Vol 47 (1) ◽  
pp. e62 ◽  
Author(s):  
Antje Appelt-Menzel ◽  
Alevtina Cubukova ◽  
Marco Metzger
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Pluripotent Stem Cells ◽  
Neurovascular Unit ◽  
Brain Barrier ◽  
Blood Brain ◽  
Culture Model ◽  
Induced Pluripotent

scholarly journals Establishment of a Human Blood-Brain Barrier Co-culture Model Mimicking the Neurovascular Unit Using Induced Pluri- and Multipotent Stem Cells

2017 ◽  
Vol 8 (4) ◽  
pp. 894-906 ◽  
Author(s):  
Antje Appelt-Menzel ◽  
Alevtina Cubukova ◽  
Katharina Günther ◽  
Frank Edenhofer ◽  
Jörg Piontek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Neurovascular Unit ◽  
Brain Barrier ◽  
Multipotent Stem Cells ◽  
Blood Brain ◽  
Culture Model

scholarly journals Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 994 ◽  
Author(s):  
Annalise Di Marco ◽  
Domenico Vignone ◽  
Odalys Gonzalez Paz ◽  
Ivan Fini ◽  
Maria Rosaria Battista ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Human Model ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent

The blood-brain barrier (BBB) is responsible for the homeostasis between the cerebral vasculature and the brain and it has a key role in regulating the influx and efflux of substances, in healthy and diseased states. Stem cell technology offers the opportunity to use human brain-specific cells to establish in vitro BBB models. Here, we describe the establishment of a human BBB model in a two-dimensional monolayer culture, derived from human induced pluripotent stem cells (hiPSCs). This model was characterized by a transendothelial electrical resistance (TEER) higher than 2000 Ω∙cm2 and associated with negligible paracellular transport. The hiPSC-derived BBB model maintained the functionality of major endothelial transporter proteins and receptors. Some proprietary molecules from our central nervous system (CNS) programs were evaluated revealing comparable permeability in the human model and in the model from primary porcine brain endothelial cells (PBECs).

scholarly journals Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Emma K. Hollmann ◽  
Amanda K. Bailey ◽  
Archit V. Potharazu ◽  
M. Diana Neely ◽  
Aaron B. Bowman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Blood Brain ◽  
Induced Pluripotent
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