Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial-Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model

2021 ◽  
Author(s):  
Yuan Li ◽  
Georg C. Terstappen ◽  
Wandong Zhang
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent ◽  
Barrier Model

scholarly journals Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 994 ◽  
Author(s):  
Annalise Di Marco ◽  
Domenico Vignone ◽  
Odalys Gonzalez Paz ◽  
Ivan Fini ◽  
Maria Rosaria Battista ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Human Model ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent

The blood-brain barrier (BBB) is responsible for the homeostasis between the cerebral vasculature and the brain and it has a key role in regulating the influx and efflux of substances, in healthy and diseased states. Stem cell technology offers the opportunity to use human brain-specific cells to establish in vitro BBB models. Here, we describe the establishment of a human BBB model in a two-dimensional monolayer culture, derived from human induced pluripotent stem cells (hiPSCs). This model was characterized by a transendothelial electrical resistance (TEER) higher than 2000 Ω∙cm2 and associated with negligible paracellular transport. The hiPSC-derived BBB model maintained the functionality of major endothelial transporter proteins and receptors. Some proprietary molecules from our central nervous system (CNS) programs were evaluated revealing comparable permeability in the human model and in the model from primary porcine brain endothelial cells (PBECs).

scholarly journals An isogenic blood-brain barrier model comprising brain endothelial cells, astrocytes, and neurons derived from human induced pluripotent stem cells

2017 ◽  
Vol 140 (6) ◽  
pp. 874-888 ◽  
Author(s):  
Scott G. Canfield ◽  
Matthew J. Stebbins ◽  
Bethsymarie Soto Morales ◽  
Shusaku W. Asai ◽  
Gad D. Vatine ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Brain Endothelial Cells ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent ◽  
Barrier Model

scholarly journals Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Emma K. Hollmann ◽  
Amanda K. Bailey ◽  
Archit V. Potharazu ◽  
M. Diana Neely ◽  
Aaron B. Bowman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Blood Brain ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document