Impact of acute inflammation on cytochromes P450 activity assessed by the Geneva Cocktail.

Pharmacotherapy in patients with comorbidity is a current issue for clinical practice. Combination of hypertension and musculoskeletal diseases can be found in 40% of outpatients, which requires simultaneous administration of different drugs. The main mechanisms of drug interactions are associated with pharmacokinetics or pharmacodynamics alterations. It has been proven that changes in drugs pharmacokinetics can be due to cytochromes P450 activity. The main symptom of musculoskeletal diseases is chronic pain, which requires long-term therapy with non-steroidal anti-inflammatory drugs (NSAIDs). The 2C19 isoenzyme takes part in metabolism of some NSAIDs. Losartan, the inhibitor of renin-angiotensinaldosterone system (RAAS), is also metabolized by the 2C9 isoenzyme and is quite often prescribed to outpatients to treat hypertension. Hence, an influence of genetic factors on efficacy and safety of antihypertensive drugs and NSAIDs combinations requires further studies.

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Evaluation of P450 monooxygenase activity in lyophilized recombinant E. coli cells compared to resting cells

AMB Express ◽

10.1186/s13568-021-01319-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Thomas Hilberath ◽

Alessandra Raffaele ◽

Leonie M. Windeln ◽

Vlada B. Urlacher

Keyword(s):

Cytochromes P450 ◽

Rhodococcus Erythropolis ◽

Resting Cells ◽

P450 Monooxygenase ◽

Monooxygenase Activity ◽

Whole Cells ◽

E Coli ◽

Redox Partner ◽

Starting Point ◽

P450 Activity

AbstractCytochromes P450 catalyze oxidation of chemically diverse compounds and thus offer great potential for biocatalysis. Due to the complexity of these enzymes, their dependency of nicotinamide cofactors and redox partner proteins, recombinant microbial whole cells appear most appropriate for effective P450-mediated biocatalysis. However, some drawbacks exist that require individual solutions also when P450 whole-cell catalysts are used. Herein, we compared wet resting cells and lyophilized cells of recombinant E. coli regarding P450-catalyzed oxidation and found out that lyophilized cells are well-appropriate as P450-biocatalysts. E. coli harboring CYP105D from Streptomyces platensis DSM 40041 was used as model enzyme and testosterone as model substrate. Conversion was first enhanced by optimized handling of resting cells. Co-expression of the alcohol dehydrogenase from Rhodococcus erythropolis for cofactor regeneration did not affect P450 activity of wet resting cells (46% conversion) but was crucial to obtain sufficient P450 activity with lyophilized cells reaching a conversion of 72% under the same conditions. The use of recombinant lyophilized E. coli cells for P450 mediated oxidations is a promising starting point towards broader application of these enzymes.

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Evaluation of Recombinant Cytochromes P450 Activity in Metabolic Pathways

Drug Metabolism and Disposition ◽

10.1124/dmd.31.1.145 ◽

2003 ◽

Vol 31 (1) ◽

pp. 145-146 ◽

Cited By ~ 3

Author(s):

Bertrand Rochat

Keyword(s):

Metabolic Pathways ◽

Cytochromes P450 ◽

P450 Activity

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Impact of Inflammation on Cytochromes P450 Activity in Pediatrics: A Systematic Review

Clinical Pharmacokinetics ◽

10.1007/s40262-021-01064-4 ◽

2021 ◽

Author(s):

Camille Lenoir ◽

Frédérique Rodieux ◽

Jules A. Desmeules ◽

Victoria Rollason ◽

Caroline F. Samer

Keyword(s):

Systematic Review ◽

Cytochromes P450 ◽

P450 Activity

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Cytochromes P450

10.1039/9781847558428 ◽

2008 ◽

Cited By ~ 13

Keyword(s):

Cytochromes P450

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Acute Inflammation of the Pancreas

Gastroenterology ◽

10.1016/s0016-5085(58)80009-8 ◽

1958 ◽

Vol 34 (3) ◽

pp. 467-475 ◽

Cited By ~ 12

Author(s):

H.L. Bockus

Keyword(s):

Acute Inflammation

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Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000620 ◽

2019 ◽

pp. 1-9

Author(s):

Samira Salem ◽

Essaid Leghouchi ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Time Course ◽

Acute Inflammation ◽

Lichen Species ◽

Paw Edema ◽

Sod Activity ◽

Edema Formation ◽

Lobaria Pulmonaria ◽

Anti Inflammatory ◽

Endogenous Antioxidant ◽

Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

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Schistosomal Appendicitis in Pregnancy

Swiss Surgery ◽

10.1024/1023-9332.8.3.121 ◽

2002 ◽

Vol 8 (3) ◽

pp. 121-122 ◽

Cited By ~ 9

Author(s):

Halkic ◽

Abdelmoumene ◽

Gintzburger ◽

Mosimann

Keyword(s):

Acute Appendicitis ◽

Schistosoma Haematobium ◽

Acute Inflammation ◽

The Body ◽

Parasitic Infections ◽

Common Complication ◽

Surgical Infection ◽

High Prevalence ◽

Potential Challenge ◽

In Pregnancy

Acute appendicitis is the most common acute surgical infection during pregnancy. Although usually pyogenic in origin, parasitic infections account for a small percentage of cases. Despite the relatively high prevalence of acute appendicitis in our environment, it is not commonly associated with schistosomiasis. We report here the association of pregnancy and appendicitis caused by Schistosoma haematobium. Schistosomiasis is very common complication of pregnancy in hyperendemic areas. Schistosome egg masses can lodge throughout the body and cause acute inflammation of the appendix, liver and spleen. Congestion of pelvic vessels during pregnancy facilitates passage of eggs into the villi and intervillous spaces, causing an inflammatory reaction. Tourism and immigration make this disease a potential challenge for practitioners everywhere.

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