Rapid identification of RNA‐interference‐based resistance to Bean golden mosaic virus in transgenic common beans via loop‐mediated isothermal amplification

Crop Science ◽  
2020 ◽  
Vol 60 (6) ◽  
pp. 3004-3012
Author(s):  
Nara Cristina Teixeira ◽  
Adriane Wendland ◽  
Maythsulene Inácio de Souza Oliveira ◽  
Livia Teixeira Duarte Brandão ◽  
Thiago Lívio Pessoa Oliveira Souza ◽  
...  
Keyword(s):  
Rna Interference ◽  
Mosaic Virus ◽  
Rapid Identification ◽  
Isothermal Amplification ◽  
Common Beans ◽  
Bean Golden Mosaic Virus ◽  
Loop Mediated Isothermal Amplification

scholarly journals Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Soybean yellow mottle mosaic virus

2016 ◽  
Vol 22 (3) ◽  
pp. 178-183
Author(s):  
Dae Hyeon Bae ◽  
Chung Youl Park ◽  
Bong-Sub Kim ◽  
Yeong-Hoon Lee ◽  
Young-Nam Yoon ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Yellow Mottle

scholarly journals Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants

2015 ◽  
Vol 31 (4) ◽  
pp. 438-440 ◽  
Author(s):  
Siwon Lee ◽  
Jin-Ho Kim ◽  
Ji-Young Choi ◽  
Won-Cheoul Jang
Keyword(s):  
Mosaic Virus ◽  
Wheat Streak Mosaic Virus ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

scholarly journals Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification

2011 ◽  
Vol 47 (No. 4) ◽  
pp. 140-148 ◽  
Author(s):  
N. Rostamkhani ◽  
A. Haghnazari ◽  
M. Tohidfar ◽  
A. Moradi
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Lamp Assay ◽  
Transgenic Cotton ◽  
Rapid Identification ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Lamp Method ◽  
Loop Mediated Isothermal Amplification ◽  
Leaf Tissues

In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was extracted from fresh leaf tissues of T<sub>2</sub> transgenic cotton containing chitinase (chi) and cry1A(b) genes. Detection of genes of interest was performed by polymerase chain reaction (PCR), LAMP and real-time PCR methods. In LAMP assay the amplification was performed after 30 min at 65&deg;C when loop primers were involved in the reaction. The involvement of loop primers decreased the time needed for amplification. By testing serial tenfold dilutions (10<sup>&ndash;1</sup> to 10<sup>&ndash;8</sup>) of the genes of interest, the detection sensitivity of LAMP was found to be 100-fold higher than that of PCR. The rapid DNA extraction method and LAMP assay can be performed within 30 min and the derived LAMP products can be directly observed as visually detectable based on turbidity in the reaction tube. The accuracy of LAMP method in the screening of transgenes was confirmed by PCR and real-time PCR. The developed method was efficient, rapid and sensitive in the screening of cotton transgenic plants. This method can be applied to any other crops.

scholarly journals Rapid identification of Aconitum plants based on loop-mediated isothermal amplification assay

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Masashi Kitamura ◽  
Akira Kazato ◽  
Tadashi Yamamuro ◽  
Hirokazu Ando ◽  
Yohei Sasaki ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay
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