Quantitative trait loci for field resistance to barley stripe rust derived from malting line 95SR316A

The Chinese wheat landrace “Gaoxianguangtoumai” (GX) has exhibited a high level of adult-plant resistance (APR) to stripe rust in the field for more than a decade. To reveal the genetic background for APR to stripe rust in GX, a set of 249 F6:8 (F6, F7, and F8) recombinant inbred lines (RILs) was developed from a cross between GX and the susceptible cultivar “Taichung 29.” The parents and RILs were evaluated for disease severity at the adult-plant stage in the field by artificial inoculation with the currently predominant Chinese Puccinia striiformis f. sp. tritici races during three cropping seasons and genotyped using the Wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,871 SNP markers finally. Two stable APR quantitative trait loci (QTL), QYr.GX-2AS and QYr.GX-7DS in GX, were detected on chromosomes 2AS and 7DS, which explained 15.5–27.0% and 11.5–13.5% of the total phenotypic variation, respectively. Compared with published Yr genes and QTL, QYr.GX-7DS and Yr18 may be the same, whereas QYr.GX-2AS is likely to be novel. Haplotype analysis revealed that QYr.GX-2AS is likely to be rare which presents in 5.3% of the 325 surveyed Chinese wheat landraces. By analyzing a heterogeneous inbred family (HIF) population from a residual heterozygous plant in an F8 generation of RIL, QYr.GX-2AS was further flanked by KP2A_36.85 and KP2A_38.22 with a physical distance of about 1.37Mb and co-segregated with the KP2A_37.09. Furthermore, three tightly linked Kompetitive allele-specific PCR (KASP) markers were highly polymorphic among 109 Chinese wheat cultivars. The results of this study can be used in wheat breeding for improving resistance to stripe rust.

Download Full-text

Introgression of quantitative trait loci (QTLs) determining stripe rust resistance in barley: an example of marker-assisted line development

Theoretical and Applied Genetics ◽

10.1007/s001220050718 ◽

1998 ◽

Vol 96 (1) ◽

pp. 123-131 ◽

Cited By ~ 105

Author(s):

T. Toojinda ◽

E. Baird ◽

A. Booth ◽

L. Broers ◽

P. Hayes ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Stripe Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Trait Loci

Download Full-text

Identification and mapping of leaf, stem and stripe rust resistance quantitative trait loci and their interactions in durum wheat

Molecular Breeding ◽

10.1007/s11032-012-9798-4 ◽

2012 ◽

Vol 31 (2) ◽

pp. 405-418 ◽

Cited By ~ 40

Author(s):

A. Singh ◽

M. P. Pandey ◽

A. K. Singh ◽

R. E. Knox ◽

K. Ammar ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Durum Wheat ◽

Stripe Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Trait Loci

Download Full-text

Quantitative trait loci associated with agronomic traits and stripe rust in winter wheat mapping population using single nucleotide polymorphic markers

Molecular Breeding ◽

10.1007/s11032-017-0704-y ◽

2017 ◽

Vol 37 (8) ◽

Cited By ~ 2

Author(s):

Ma Yu ◽

Hong Zhang ◽

Xin-Li Zhou ◽

Da-Bin Hou ◽

Guo-Yue Chen

Keyword(s):

Quantitative Trait Loci ◽

Winter Wheat ◽

Stripe Rust ◽

Quantitative Trait ◽

Agronomic Traits ◽

Mapping Population ◽

Polymorphic Markers ◽

Single Nucleotide Polymorphic ◽

Single Nucleotide ◽

Trait Loci

Download Full-text

Mapping of Stripe Rust and Leaf Rust Resistance Quantitative Trait Loci in the Chinese Spring Wheat Line Mianyang351-15

Phytopathology ◽

10.1094/phyto-08-19-0316-r ◽

2020 ◽

Vol 110 (5) ◽

pp. 1074-1081 ◽

Cited By ~ 1

Author(s):

Takele Weldu Gebrewahid ◽

Yue Zhou ◽

Peipei Zhang ◽

Yong Ren ◽

Pu Gao ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Stripe Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Chinese Spring Wheat ◽

Ril Population ◽

Trait Loci ◽

Rust Severity

Stripe rust and leaf rust cause wheat yield losses of up to 70% worldwide. The employment of resistant cultivars is the major method to reduce losses from these diseases. The objective of this study was to detect quantitative trait loci (QTL) for stripe rust and leaf rust resistance in 150 F6 recombinant inbred lines (RIL) derived from a cross between Mianyang351-15 and Zhengzhou 5389. Both parents and the RIL population were genotyped with the Wheat55K single nucleotide polymorphism (SNP) array and simple sequence repeat markers, and phenotyped for stripe rust severity at Mianyang in Sichuan Province and Baoding in Hebei Province, and for leaf rust severity at Zhoukou in Henan Province and at Baoding in 2014 to 2017 cropping seasons. Seven and four QTL all contributed from Mianyang351-15 were identified for resistance to stripe rust and leaf rust, respectively. Four of these QTL on chromosomes 1BL, 2AS, 2DS, and 7BL conferred resistance to both stripe rust and leaf rust. The QTL on 1BL, 2AS, and 7BL were identified as Lr46/Yr29, Lr37/Yr17, and Lr68, respectively. QYr.hbau-2DS/QLr.hbau-2DS was detected at similar positions to previously reported loci. QYr.hbau-1DL, QYr.hbau-3AS, and QYr.hbau-3DL are likely to be new. Combined effects of QTL in the RIL population indicated RIL combining all QTL had the highest resistance level compared with those of lower numbers or no QTL. These QTL, with their closely linked SNP markers, are applicable for marker-assisted breeding and candidate gene discovery.

Download Full-text

Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410

International Journal of Molecular Sciences ◽

10.3390/ijms21020478 ◽

2020 ◽

Vol 21 (2) ◽

pp. 478 ◽

Cited By ~ 3

Author(s):

Yan Liu ◽

Yanmin Qie ◽

Xing Li ◽

Meinan Wang ◽

Xianming Chen

Keyword(s):

High Temperature ◽

Quantitative Trait Loci ◽

Stripe Rust ◽

Spring Wheat ◽

Quantitative Trait ◽

Puccinia Striiformis ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Wheat Landrace ◽

Trait Loci

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Genetic resistance is the best strategy for control of the disease. Spring wheat landrace PI 181410 has shown high level resistance to stripe rust. The present study characterized the landrace to have both race-specific all-stage resistance and nonrace-specific high-temperature adult-plant (HTAP) resistance. To map quantitative trait loci (QTL) for the resistance in PI 181410, it was crossed with Avocet S (AvS), from which a recombinant inbred line population was developed. The F5–F8 populations were consecutively phenotyped for stripe rust response in multiple field environments under natural Pst infection, and the F7 population was phenotyped in seedlings at low temperature and in adult-plant stage with selected Pst races in the greenhouse. The F7 population was genotyped using the 90K wheat SNP chip. Three QTL, QYrPI181410.wgp-4AS, QYrPI181410.wgp-4BL, and QYrPI181410.wgp-5BL.1, from PI 181410 for all-stage resistance, were mapped on chromosome arms 4AS, 4BL, and 5BL, respectively. Four QTL, QYrPI181410.wgp-1BL, QYrPI181410.wgp-4BL, QYrPI181410.wgp-5AS, and QYrPI181410.wgp-5BL.2, were identified from PI 181410 for HTAP resistance and mapped to 1BL, 4BL, 5AS, and 5BL, respectively. Two QTL with minor effects on stripe rust response were identified from AvS and mapped to 2BS and 2BL. Four of the QTL from PI 181410 and one from AvS were potentially new. As the 4BL QTL was most effective and likely a new gene for stripe rust resistance, three kompetitive allele specific PCR (KASP) markers were developed for incorporating this gene into new wheat cultivars.

Download Full-text