scholarly journals Spatial topography of a pericentromeric region (1q12) in hemopoietic cells studied by in situ hybridization and confocal microscopy

Cytometry ◽  
1990 ◽  
Vol 11 (5) ◽  
pp. 570-578 ◽  
Author(s):  
H. van Dekken ◽  
A. van Rotterdam ◽  
R. R. Jonker ◽  
H. T. M. van der Voort ◽  
G. J. Brakenhoff ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Confocal Microscopy ◽  
Pericentromeric Region ◽  
Hemopoietic Cells ◽  
Spatial Topography

Three-Dimensional Laser-Scanning Confocal Microscopy of In Situ Hybridization in the Skin

1994 ◽  
Vol 16 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Stephen E. Mahoney ◽  
Stephen W. Paddock ◽  
Louis C. Smith ◽  
Dorothy E. Lewis ◽  
Madeleine Duvic
Keyword(s):  
In Situ Hybridization ◽  
Confocal Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy

Detecting Early Apoptosis in Whole Epithelial Sheets with a Caspase 3 Substrate, Phiphilux and Annexes V Using Confocal Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 600-601
Author(s):  
Kathy K. H. Svoboda
Keyword(s):  
In Situ Hybridization ◽  
Confocal Microscopy ◽  
General Principle ◽  
Caspase 3 ◽  
Living Cells ◽  
Corneal Epithelial ◽  
Early Apoptosis ◽  
Epithelial Sheets ◽  
Conventional Light Microscope

Many reagents have been developed recently to label living cells with substrates that will become fluorescent if an enzyme is active. The general principle is that the substrate will be taken up by living cells then detected only if the enzyme is active. These substrates work well with isolated individual cells, however, more difficulty can be encountered when studying whole tissues. Problems can range from substrate penetration into whole tissues to being able to detect the label effectively. We have used the chicken corneal epithelia for many studies, but the tissue is to thick to view with a conventional light microscope, therefore we have developed techniques using laser confocal microscopes to view this tissue in with a variety of techniques including in situ hybridization, immunohistochemistry, and vital dyes/stains.Whole embryonic corneal epithelial sheets can be isolated without the basal lamina.

The pericentromeric heterochromatin of the grass Zingeria biebersteiniana (2n = 4) is composed of Zbcen1-type tandem repeats that are intermingled with accumulated dispersedly organized sequences

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 955-961 ◽  
Author(s):  
Verity A Saunders ◽  
Andreas Houben
Keyword(s):  
In Situ Hybridization ◽  
Tandem Repeat ◽  
Tandem Repeats ◽  
Repetitive Sequences ◽  
Pericentromeric Region ◽  
Pericentromeric Heterochromatin ◽  
Repeat Family ◽  
Dna Reassociation ◽  
Copy Dna

DNA reassociation and hydroxyapatite chromatography were used to isolate high-copy DNA of the grass Zingeria biebersteiniana (2n = 4). In situ hybridization demonstrated that the DNA isolated was enriched for pericentromere-specific repetitive sequences. One abundant pericentromere-specific component is the differentially methylated tandem-repeat family Zbcen1. Other sequences isolated, Zb46 and Zb47A, are dispersed and display similarity to parts of the gypsy- and copia-like retrotransposable elements of other grasses. In situ hybridization with the copia-like sequence Zb47A resulted in dispersed labelling along the chromosome arms, with a significant signal accumulation in the pericentromeric region of all chromosomes. It is concluded that the pericentromeric heterochromatin of Z. biebersteiniana is composed of members of the Zbcen1 tandem repeat family and that these tandem arrays are intermingled with accumulated putative copia-like retrotransposon sequences. An observed Rabl interphase orientation suggests that the length of the chromosomes rather than the genome size is the determining factor of the Rabl phenomenon.Key Words: centromere, heterochromatin, tandemly repeated DNA, retrotransposon-like, DNA reassociation.

Complementary patterns of expression of c-ets 1, c-myb and c-myc in the blood-forming system of the chick embryo

Development ◽  
1989 ◽  
Vol 107 (2) ◽  
pp. 265-274 ◽  
Author(s):  
B. Vandenbunder ◽  
L. Pardanaud ◽  
T. Jaffredo ◽  
M.A. Mirabel ◽  
D. Stehelin
Keyword(s):  
In Situ Hybridization ◽  
Chick Embryo ◽  
Temporal Distribution ◽  
Dna Binding Proteins ◽  
Spatial And Temporal Distribution ◽  
Specific Expression ◽  
Hemopoietic Cells ◽  
Cellular Oncogenes ◽  
Mesodermal Lineage

We have used in situ hybridization to study the spatial and temporal distribution of the transcription of three cellular oncogenes encoding DNA-binding proteins, c-ets 1, c-myb and c-myc during the development of the chick embryo. c-ets 1 mRNA expression appears linked to the mesodermal lineage and is strongly expressed in early endothelia; it subsequently becomes restricted to small vessel endothelia. Hemopoietic cells in extraembryonic blood islands at E2 express c-ets 1, while intraembryonic hemopoietic cells in aortic clusters (E3) and paraaortic foci (E6) express c-myb. c-myc transcripts are detected in cells undergoing hemopoiesis in both these extraembryonic and intraembryonic sites. Outside the blood-forming system, c-myc is transcribed in a large variety of cells; c-ets 1 displays tissue-specific expression in groups of mesodermal cells engaged in morphogenetic processes and appears excluded from all epithelia; finally the expression of c-myb is the most tightly linked to hemopoietic cells. In any case, it is clear that these three oncogenes display complementary expression in endothelial and hemopoietic cells where their patterns are modulated in relationship to multiplication and differentiation.

scholarly journals Sex chromosome composition revealed in Characidium fishes (Characiformes: Crenuchidae) by molecular cytogenetic methods

Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Marlon Pazian ◽  
Claudio Oliveira ◽  
Fausto Foresti
Keyword(s):  
In Situ Hybridization ◽  
Sequence Analysis ◽  
Sex Chromosome ◽  
Repetitive Sequences ◽  
Pericentromeric Region ◽  
W Chromosome ◽  
Degenerate Oligonucleotide ◽  
Clone Sequence ◽  
Positive Hybridization

AbstractThe W chromosome of the fishes Characidium cf. fasciatum, Characidium sp. and Characidium cf. gomesi is heterochromatic, as is usually seen in most Characidium species. Samples of W-chromatin were collected by mechanical microdissection and amplified by DOP-PCR (degenerate oligonucleotide-primed polymerase chain reaction), to be used as painting probes (DCg and CgW) and for sequence analysis. FISH (fluorescence in situ hybridization) with DCg probe painted the whole W chromosome, the pericentromeric region of Z chromosomes and the terminal region of B chromosomes. DOP-PCR-generated fragments were cloned, sequenced and tested by in situ hybridization, but only CgW4 produced positive hybridization signals. Clone sequence analysis recovered seven distinct sequences, of which six did not reveal any similarity to other known sequences in the GenBank or GIRI databases. Only CgW9 clone sequence was recognized as probably derived from a Helitron-transposon similar to that found in the genome of the zebrafish Danio rerio. Our results show that the composition of Characidium’s W chromosome does seem rich in repetitive sequences as well as other W chromosomes found in several species with a ZW sex-determining mechanism.

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