scholarly journals An X-linked Myh11-CreERT2 mouse line resulting from Y to X chromosome-translocation of the Cre allele

genesis ◽  
2017 ◽  
Vol 55 (9) ◽  
pp. e23054 ◽  
Author(s):  
Mingmei Liao ◽  
Junmei Zhou ◽  
Fen Wang ◽  
Yasmin H. Ali ◽  
Kelvin L. Chan ◽  
...  
Keyword(s):  
X Chromosome ◽  
Chromosome Translocation ◽  
Mouse Line

Abstract 309: A Female Myh11-CreER T2 Mouse Line Resulting from Y to X Chromosome-translation

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Junmei Zhou ◽  
Mingmei Liao ◽  
Pu Yang ◽  
Yasmin Ali ◽  
Fei Zou ◽  
...  
Keyword(s):  
Y Chromosome ◽  
X Chromosome ◽  
Gene Deletion ◽  
Bayes Factor ◽  
Vascular Diseases ◽  
Chromosome Translocation ◽  
Chromosome Location ◽  
Mouse Line ◽  
Male And Female ◽  
Female Mice

The Myh11-CreER T2 mouse line ( Cre +/ ) provides a powerful tool for studying the role of smooth muscle cells (SMCs) in the pathogenesis of vascular diseases. The Cre allele was initially inserted into Y chromosome, and thus excluded its inheritance by female mice. However, a number of vascular diseases exhibit sexual dimorphism in development and progression, and the underlying mechanisms may involve regulation of SMC biology by the dosage-effects of sex-linked genes and gender. A Cre line that induces SMC-specific gene deletion in female mice would complement the existing Cre +/ line to address this critical issue. Our lab established the Cre +/ colony with one male ancestor and it spontaneously separated to two subcolonies during subsequent breeding. One subcolony produced only male Cre +/ progenies, but the other produced both male and female Cre +/ progenies, indicating chromosome-translocation of the Cre allele. In view of this observation, we mapped the chromosome-location of the Cre allele for these subcolonies with cross-breeding experiments. The 1 st experiment crossed two Cre +/ males from the first subcolony with four Cre -/ females. Of the produced progenies, all males ( n =9) carried and all females ( n =22) did not carry the Cre allele, which favors Y chromosome- ( Y Cre+ ) over autosome-location of the Cre allele in this subcolony (Bayes Factor>1000). In the 2 nd experiment, a Cre +/ female from the second subcolony was bred with a Cre -/ male. Then, a Cre +/ male progeny of these breeders was mated with a Cre -/ female. The former breeding pair exhibited equal inheritance of the Cre allele by its male and female offspring. The latter breeding pair showed that all female progenies ( n =8) did, but all male progenies ( n =4) did not, carry the Cre allele. The data favors X chromosome- ( X Cre+ ) over autosome-location of the Cre allele in this subcolony (Bayes Factor>1000). To assess the recombination driven by Cre +/ allele with different chromosome-location, we crossed X Cre+ and Y Cre+ strains with R26R reporter mice. Robust Cre activity (x-gal staining) was observed in vascular SMCs of Y Cre+ and X Cre+ mice. The X Cre+ mice generated via Y to X chromosome-translocation thus represents a novel strain that is suitable for inducible gene deletion in SMCs of female mice.

Differential expression of α-fetoprotein genes on the inactive X chromosome in extraembryonic and somatic tissues of a transgenic mouse line

Nature ◽  
1986 ◽  
Vol 319 (6050) ◽  
pp. 224-226 ◽  
Author(s):  
R. Krumlauf ◽  
V. M. Chapman ◽  
R. E. Hammer ◽  
R. Brinster ◽  
S. M. Tilghman
Keyword(s):  
Differential Expression ◽  
Transgenic Mouse ◽  
X Chromosome ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Inactive X Chromosome ◽  
Somatic Tissues

scholarly journals Sex chromosomal dimorphisms narrated by X-chromosome translocation in a spiny frog (Quasipaa boulengeri)

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
Xiuyun Yuan ◽  
Yun Xia ◽  
Xiaomao Zeng
Keyword(s):  
X Chromosome ◽  
Chromosome Translocation

Y-to-X chromosome translocation observed in two generations

1980 ◽  
Vol 55 (1) ◽  
pp. 39-42 ◽  
Author(s):  
H. O. �kesson ◽  
B. Hagberg ◽  
J. Wahlstr�m
Keyword(s):  
X Chromosome ◽  
Chromosome Translocation ◽  
Two Generations

scholarly journals X-autosome and X-Y translocations in female carriers: X-chromosome inactivation easily detectable by 5-ethynyl-2-deoxyuridine (EdU)

2017 ◽  
Vol 20 (1) ◽  
pp. 87-90 ◽  
Author(s):  
M Donat ◽  
A Louis ◽  
K Kreskowski ◽  
M Ziegler ◽  
A Weise ◽  
...  
Keyword(s):  
X Chromosome ◽  
Chromosome Translocation ◽  
X Chromosome Inactivation ◽  
Chromosome Inactivation ◽  
Correct Interpretation ◽  
Cell Level ◽  
Diagnostic Use ◽  
Autosome Translocation ◽  
Skewed X Chromosome Inactivation ◽  
Female Carriers

Abstract Here we report one new case each of an X-autosome translocation (maternally derived), and an X-Y-chromosome translocation. Besides characterizing the involved breakpoints and/or imbalances in detail by molecular cyto-genetics, also skewed X-chromosome inactivation was determined on single cell level using 5-ethynyl-2-deoxyuridine (EdU). Thus, we confirmed that the recently suggested EdU approach can be simply adapted for routine diagnostic use. The latter is important, as only by knowing the real pattern of the skewed X-chromosome inactivation, correct interpretation of obtained results and subsequent reliable genetic counseling, can be done.

Adding the heterochromatic YL arm to an X chromosome reduces reproductive fitnesses in Drosophila melanogaster: implications for the evolution of rDNA, heterochromatin, and reproductive isolation

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 340-347 ◽  
Author(s):  
R. Frankham
Keyword(s):  
Drosophila Melanogaster ◽  
Reproductive Isolation ◽  
X Chromosome ◽  
Sex Chromosome ◽  
Reproductive Fitness ◽  
Chromosome Translocation ◽  
Translocation Chromosome ◽  
X Chromosomes ◽  
The Face ◽  
Over Time

For X–Y exchange to be of importance in the coevolution of X and Y rDNA, there must be a mechanism to maintain cytologically normal X chromosomes in the face of continual infusions of X.YL chromosomes produced by X–Y exchanges. Replicated populations were founded with different frequencies of isogenic X and X.YL chromosomes. The X.YL chromosome declined in frequency over time in all lines. Relative fitnesses, estimated from chromosome frequency trajectories, were 0.40, 1.01, and 1.0 for X.YL/X.YL, X.YL/X, and X/X females and 0.75 and 1.0 for X.YL/Y and X/Y males, respectively. The equilibrium frequency for the X.YL chromosome due to the balance between X–Y exchange and selection was predicted to be 4–16 × 10−4. The results strengthen the evidence for the involvement of X–Y exchange in the coevolution of X and Y rDNA arrays. Conditions for the evolution of reproductive isolation by sex-chromosome translocation are much less probable than previously supposed since the X.YL translocation chromosome is at a selective disadvantage to cytologically normal X chromosomes. Additional heterochromatin was not neutral but was only deleterious beyond a threshold, as one dose of the heterochromatic XL arm did not reduce female reproductive fitness, but two doses did.Key words: Drosophila, rRNA, heterochromatin, fitness, speciation.

Evidence for X/X chromosome translocation in humans

1969 ◽  
Vol 33 (2) ◽  
pp. 117-124 ◽  
Author(s):  
ANIL K. SINHA ◽  
JAMES J. NORA
Keyword(s):  
X Chromosome ◽  
Chromosome Translocation

Interstitial deletion of 13q and a 13;X chromosome translocation results in partial trisomy 13 and bilateral retinoblastoma

2003 ◽  
Vol 24 (3) ◽  
pp. 175-180 ◽  
Author(s):  
David Dries ◽  
Katrina Baca ◽  
Lisa Truss ◽  
Sheila Dobin
Keyword(s):  
X Chromosome ◽  
Partial Trisomy ◽  
Chromosome Translocation ◽  
Interstitial Deletion ◽  
Trisomy 13 ◽  
Bilateral Retinoblastoma

scholarly journals Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line

2010 ◽  
Vol 11 (1) ◽  
pp. 58 ◽  
Author(s):  
Bowen Yan ◽  
Defa Li ◽  
Kemian Gou
Keyword(s):  
Transgenic Mouse ◽  
X Chromosome ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Random Integration ◽  
Foreign Dna
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