Differential expression of α-fetoprotein genes on the inactive X chromosome in extraembryonic and somatic tissues of a transgenic mouse line

Nature ◽  
1986 ◽  
Vol 319 (6050) ◽  
pp. 224-226 ◽  
Author(s):  
R. Krumlauf ◽  
V. M. Chapman ◽  
R. E. Hammer ◽  
R. Brinster ◽  
S. M. Tilghman
Keyword(s):  
Differential Expression ◽  
Transgenic Mouse ◽  
X Chromosome ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Inactive X Chromosome ◽  
Somatic Tissues

scholarly journals Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line

2010 ◽  
Vol 11 (1) ◽  
pp. 58 ◽  
Author(s):  
Bowen Yan ◽  
Defa Li ◽  
Kemian Gou
Keyword(s):  
Transgenic Mouse ◽  
X Chromosome ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Random Integration ◽  
Foreign Dna

scholarly journals A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals

Function ◽  
2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Nelly Redolfi ◽  
Elisa Greotti ◽  
Giulia Zanetti ◽  
Tino Hochepied ◽  
Cristina Fasolato ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Brain Slices ◽  
Resonance Energy ◽  
Mouse Line ◽  
Isolated Cells ◽  
Genomic Locus ◽  
Transgenic Mouse Line

AbstractMitochondria play a key role in cellular calcium (Ca2+) homeostasis. Dysfunction in the organelle Ca2+ handling appears to be involved in several pathological conditions, ranging from neurodegenerative diseases, cardiac failure and malignant transformation. In the past years, several targeted green fluorescent protein (GFP)-based genetically encoded Ca2+ indicators (GECIs) have been developed to study Ca2+ dynamics inside mitochondria of living cells. Surprisingly, while there is a number of transgenic mice expressing different types of cytosolic GECIs, few examples are available expressing mitochondria-localized GECIs, and none of them exhibits adequate spatial resolution. Here we report the generation and characterization of a transgenic mouse line (hereafter called mt-Cam) for the controlled expression of a mitochondria-targeted, Förster resonance energy transfer (FRET)-based Cameleon, 4mtD3cpv. To achieve this goal, we engineered the mouse ROSA26 genomic locus by inserting the optimized sequence of 4mtD3cpv, preceded by a loxP-STOP-loxP sequence. The probe can be readily expressed in a tissue-specific manner upon Cre recombinase-mediated excision, obtainable with a single cross. Upon ubiquitous Cre expression, the Cameleon is specifically localized in the mitochondrial matrix of cells in all the organs and tissues analyzed, from embryos to aged animals. Ca2+ imaging experiments performed in vitro and ex vivo in brain slices confirmed the functionality of the probe in isolated cells and live tissues. This new transgenic mouse line allows the study of mitochondrial Ca2+ dynamics in different tissues with no invasive intervention (such as viral infection or electroporation), potentially allowing simple calibration of the fluorescent signals in terms of mitochondrial Ca2+ concentration ([Ca2+]).

Transgenic Mouse Line with Green-fluorescent Protein-labeled Centrin 2 allows Visualization of the Centrosome in Living Cells

2004 ◽  
Vol 13 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Holden Higginbotham ◽  
Stephanie Bielas ◽  
Teruyuki Tanaka ◽  
Joseph G. Gleeson
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Living Cells ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Green Fluorescent

The transgenic mouse line Igsf9- eGFP allows targeted stimulation of inferior olive efferents

2018 ◽  
Vol 296 ◽  
pp. 84-92 ◽  
Author(s):  
Christina Pätz ◽  
Simone Brachtendorf ◽  
Jens Eilers
Keyword(s):  
Transgenic Mouse ◽  
Inferior Olive ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Stimulation Of

scholarly journals Generation of an inducible RPE-specific Cre transgenic-mouse line

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207222 ◽  
Author(s):  
Sandra Schneider ◽  
Nathan Hotaling ◽  
Maria Campos ◽  
Sarita Rani Patnaik ◽  
Kapil Bharti ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Line ◽  
Transgenic Mouse Line

Troponin T isoforms modulate calcium dependence of the kinetics of the cross-bridge cycle: studies using a transgenic mouse line

2002 ◽  
Vol 405 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Sarah M MacFarland ◽  
Jian-Ping Jin ◽  
Frank V Brozovich
Keyword(s):  
Transgenic Mouse ◽  
Troponin T ◽  
Mouse Line ◽  
Calcium Dependence ◽  
Transgenic Mouse Line ◽  
Cross Bridge ◽  
The Cross ◽  
Kinetics Of

scholarly journals A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0129934 ◽  
Author(s):  
Stefanie Besser ◽  
Marit Sicker ◽  
Grit Marx ◽  
Ulrike Winkler ◽  
Volker Eulenburg ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Gabaergic Neurons ◽  
Mouse Line ◽  
Red Fluorescent Protein ◽  
Transgenic Mouse Line

scholarly journals Nas transgenic mouse line allows visualization of Notch pathway activity in vivo

genesis ◽  
2006 ◽  
Vol 44 (6) ◽  
pp. 277-286 ◽  
Author(s):  
Céline Souilhol ◽  
Sarah Cormier ◽  
Marie Monet ◽  
Sandrine Vandormael-Pournin ◽  
Anne Joutel ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Notch Pathway ◽  
Mouse Line ◽  
Pathway Activity ◽  
Transgenic Mouse Line

scholarly journals Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63029 ◽  
Author(s):  
Lucie Klimova ◽  
Jitka Lachova ◽  
Ondrej Machon ◽  
Radislav Sedlacek ◽  
Zbynek Kozmik
Keyword(s):  
Transgenic Mouse ◽  
Gene Deletion ◽  
Mouse Line ◽  
Transgenic Mouse Line

scholarly journals Transmission of tauopathy strains is independent of their isoform composition

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhuohao He ◽  
Jennifer D. McBride ◽  
Hong Xu ◽  
Lakshmi Changolkar ◽  
Soo-jung Kim ◽  
...  
Keyword(s):  
Human Brain ◽  
Transgenic Mouse ◽  
Mouse Line ◽  
Cell Type ◽  
Transgenic Mouse Line ◽  
Adult Human ◽  
Tau Isoforms ◽  
Underlying Mechanisms ◽  
Mouse Lines

AbstractThe deposition of pathological tau is a common feature in several neurodegenerative tauopathies. Although equal ratios of tau isoforms with 3 (3R) and 4 (4R) microtubule-binding repeats are expressed in the adult human brain, the pathological tau from different tauopathies have distinct isoform compositions and cell type specificities. The underlying mechanisms of tauopathies are unknown, partially due to the lack of proper models. Here, we generate a new transgenic mouse line expressing equal ratios of 3R and 4R human tau isoforms (6hTau mice). Intracerebral injections of distinct human tauopathy brain-derived tau strains into 6hTau mice recapitulate the deposition of pathological tau with distinct tau isoform compositions and cell type specificities as in human tauopathies. Moreover, through in vivo propagation of these tau strains among different mouse lines, we demonstrate that the transmission of distinct tau strains is independent of strain isoform compositions, but instead intrinsic to unique pathological conformations.

Sign in / Sign up

Export Citation Format

Share Document