Primordial germ cell‐specific expression of
            eGFP
            in transgenic chickens

Genetic modification of the chicken in terms of gene addition is now robust and efficient. Transgenes can be introduced by injection of lentiviral vectors into chick embryos or by transfection of transposon vectors into embryos or primordial germ cells in vitro. Lentiviral vectors are limited in the size of transgene they can incorporate but we have generated several different transgenic lines using HIV-derived vectors and have observed high levels of transgene expression and tissue-specific expression using regulatory sequences from several genes. M. McGrew (The Roslin Institute) has established primordial germ cell lines and effective methods for transfection with piggyBac and Tol2 transposon vectors. The primordial cells are injected into chick embryos where they populate the developing gonads and contribute to the germline in mature birds. The availability of primordial germ cell lines will also form the basis of using artificial site-specific nucleases for gene knockout and potentially gene targeting in the chicken. These technologies facilitate the application of transgenesis in the chicken for basic research and for potential applications in poultry breeding. The chick embryo is an invaluable model for studying vertebrate development as the embryos can be accessed in ovo or in culture at the earlier stages of development. Embryos can be transfected with transgenes by electroporation and manipulated to study many aspects of development. We are developing transgenic chickens in which fluorescent protein reporters are expressed either ubiquitously or in targeted cell types. These form the basis of novel tools for increasing the value of the chick embryo in studying development. We provide fertile eggs from these lines to other research groups and are investigating the development of macrophages using a macrophage-targeted reporter. The potential for the use of genetic modification to be used in poultry breeding can now be explored. Commercial poultry production is challenged by several major pathogens including avian influenza. Flocks can be protected by good biosecurity measures and/or vaccination but vaccination is not always effective. It may be possible to add novel genes to the chicken genome targeting avian influenza virus replication. We are developing this approach (with L. Tiley, Cambridge University) and have generated transgenic chickens that do not transmit avian influenza when directly infected with H5N1 virus.

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Faculty Opinions recommendation of Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717959616.793465121 ◽

2012 ◽

Author(s):

John Gurdon ◽

Kei Miyamoto

Keyword(s):

Germ Cell ◽

Primordial Germ Cell

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Faculty Opinions recommendation of Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725381857.793512105 ◽

2015 ◽

Author(s):

Florence Marlow

Keyword(s):

Germ Cell ◽

Expression Profile ◽

Primordial Germ Cell ◽

Cell Commitment

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AYAM LEHER GUNDUL: SEJARAH, KARAKTERISTIK, DAN KONSERVASI

Majalah Ilmiah Peternakan ◽

10.24843/mip.2020.v23.i01.p04 ◽

2020 ◽

Vol 23 (1) ◽

pp. 22

Author(s):

Kostaman T.

Keyword(s):

Germ Cell ◽

Primordial Germ Cell

Ayam leher gundul adalah ayam asli/lokal Indonesia yang belum banyak diketahui informasinya dalam halsejarah, potensi, dan karakteristik; sehingga belum dimanfaatkan secara optimal. Ciri yang paling menonjoldari ayam leher gundul adalah tidak mempunyai bulu dibagian lehernya. Untuk mempertahankannya, metode konservasi yang telah dilakukan oleh Balai Penelitian Ternak (Balitnak) adalah dengan memelihara ternak hidup, akan tetapi dengan keterbatasan sarana dan prasarana tidak dapat dilanjutkan. Data performan ayam leher gundul yang dipelihara di Balitnak sudah terkumpul dan ditabulasi. Alternatif konservasi yang dilakukan oleh Balitnak untuk mempertahankan plasma nutfah ayam leher gundul adalah berupa primordial germ cell (PGC) yang dibekukan pada suhu -196 oC yang sewaktu-waktu dibutuhkan dapat ditransfer ke embrio resipien. Sumber PGC yang di koleksi adalah dari darah dan gonad embrio. Berdasarkan potensi yang dimilikinya, ayam leher gundul memiliki performan yang baik, sehingga dapat dimanfaatkan sebagai ayam lokal penghasil daging dan telur. Tulisan ini diharapkan dapat bermanfaat sebagai data pendukung bagi upaya pelestarian dan pengembangan ayam-ayam lokal yang ada di Indonesia, khususnya ayam leher gundul dan umumnya ayam-ayam lokal Indonesia lainnya.

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