Genetic and antigenic requirements for induction of T helper cells for anti-Sendai virus-specific antibody production

Mouse mammary tumor virus (MMTV[SW]) encodes a superantigen expressed by infected B cells. It evokes an antibody response specific for viral envelope protein, indicating selective activation of antigen-specific B cells. The response to MMTV(SW) in draining lymph nodes was compared with the response to haptenated chicken gamma globulin (NP-CGG) using flow cytometry and immunohistology. T cell priming occurs in both responses, with T cells proliferating in association with interdigitating dendritic cells in the T zone. T cell proliferation continues in the presence of B cells in the outer T zone, and B blasts then undergo exponential growth and differentiation into plasma cells in the medullary cords. Germinal centers develop in both responses, but those induced by MMTV(SW) appear later and are smaller. Most T cells activated in the T zone and germinal centers in the MMTV(SW) response are superantigen specific and these persist for weeks in lymph nodes draining the site MMTV(SW) injection; this contrasts with the selective loss of superantigen-specific T cells from other secondary lymphoid tissues. The results indicate that this viral superantigen, when expressed by professional antigen-presenting cells, drives extrafollicular and follicular B cell differentiation leading to virus-specific antibody production.

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Virus-specific antibody production and polyclonal B-cell activation in the intestinal mucosa of HIV-infected individuals

AIDS ◽

10.1097/00002030-199507000-00005 ◽

1995 ◽

Vol 9 (7) ◽

pp. 695-700 ◽

Cited By ~ 27

Author(s):

Kristina Eriksson ◽

Anders Kilander ◽

Lars Hagberg ◽

Gunnar Norkrans ◽

Jan Holmgren ◽

...

Keyword(s):

B Cell ◽

Intestinal Mucosa ◽

Antibody Production ◽

Specific Antibody ◽

Cell Activation ◽

B Cell Activation ◽

Virus Specific Antibody ◽

Specific Antibody Production

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Peripheral T follicular helper cells from H1N1/09 vaccine nonresponders fail to induce antigen-specific antibody production in vitro

Retrovirology ◽

10.1186/1742-4690-9-s2-p277 ◽

2012 ◽

Vol 9 (S2) ◽

Author(s):

A Parmigiani ◽

S Pallikkuth ◽

SY Silva ◽

M Fischl ◽

R Pahwa ◽

...

Keyword(s):

Antibody Production ◽

Specific Antibody ◽

T Follicular Helper Cells ◽

Helper Cells ◽

Follicular Helper ◽

Specific Antibody Production

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Induction of Virus-Specific Antibody Production by Lamina Propria Lymphocytes following Intramuscular Inoculation with Rotavirus

The Journal of Infectious Diseases ◽

10.1093/infdis/172.3.874 ◽

1995 ◽

Vol 172 (3) ◽

pp. 874-878 ◽

Cited By ~ 22

Author(s):

S. E. Coffin ◽

M. Klinek ◽

P. A. Offit

Keyword(s):

Lamina Propria ◽

Antibody Production ◽

Specific Antibody ◽

Intramuscular Inoculation ◽

Virus Specific Antibody ◽

Specific Antibody Production ◽

Lamina Propria Lymphocytes

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Nasal-Associated Lymphoid Tissue Is a Site of Long-Term Virus-Specific Antibody Production following Respiratory Virus Infection of Mice

Journal of Virology ◽

10.1128/jvi.75.11.5416-5420.2001 ◽

2001 ◽

Vol 75 (11) ◽

pp. 5416-5420 ◽

Cited By ~ 64

Author(s):

Bin Liang ◽

Lisa Hyland ◽

Sam Hou

Keyword(s):

Virus Infection ◽

Antibody Production ◽

Specific Antibody ◽

Immunoglobulin A ◽

Influenza Virus Infection ◽

Lymphoid Tissues ◽

Respiratory Pathogens ◽

Virus Specific Antibody ◽

Specific Antibody Production

ABSTRACT Nasal immunoglobulin A provides an initial defense against inhaled respiratory pathogens. However, it is not known whether the nasal-associated lymphoid tissues (NALT) are able to mount an effective long-lasting pathogen-specific immune response, nor is it known whether functional differences exist between the organized NALT (O-NALT) and the diffuse NALT lining the nasal passages (D-NALT). Here we show that although both the O-NALT and the D-NALT are capable of producing virus-specific antibody in response to influenza virus infection, the frequency of specific antibody-forming cells in the D-NALT is much greater than the frequency observed in the O-NALT. Furthermore, we show that the D-NALT but not the O-NALT is the site of long-term virus-specific humoral immunity which lasts for the life of the animal. These results indicate that the D-NALT is not only the major effector site of the NALT but also the site of local long-term specific antibody production.

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Suppression of IgE antibody production in SJL mice. IV. Interaction of primed and unprimed T cells.

Journal of Experimental Medicine ◽

10.1084/jem.150.3.507 ◽

1979 ◽

Vol 150 (3) ◽

pp. 507-516 ◽

Cited By ~ 9

Author(s):

T Itaya ◽

Z Ovary

Keyword(s):

T Cells ◽

Antibody Production ◽

T Helper Cells ◽

High Titer ◽

Sublethal Dose ◽

T Helper ◽

Spleen Cells ◽

Ige Antibody ◽

Helper Cells ◽

Transfer Method

The mechanism of selective anti-hapten IgE antibody production was studied in SJL mice. Using an adoptive transfer method of spleen cells into syngeneic recipients irradiated with a sublethal dose of 600 rads, it was demonstrated that for the suppression of anti-dinitrophenyl (DNP) IgE antibody production the interaction of two subsets of T cells is necessary. DNP-primed B cells and carrier-primed T helper cells are taken from donors primed with small amounts of DNP-carrier conjugates. Without injection of other cells, high titer and persistent anti-DNP antibodies are produced in the recipients. The two subsets of T cells that are active in suppression of IgE are taken from two types of donors: one donor is immunized (hyperprimed) with larger amounts of carrier protein twice, the other is an unprimed donor. The carrier for hyperpriming the first type of donor may be unrelated to the carrier used for priming the helper T cells. To bring about anti-DNP IgE suppression it is necessary that the animals should be challenged with the same DNP-carrier conjugate used for priming the B and T helper cells. If the hyperprimed donors were immunized with a heterologous, unrelated carrier, then this heterologous unconjugated carrier must also be injected together with the homologous DNP-carrier conjugate. In these conditions, anti-DNP IgE antibody production is suppressed, but the production of anti-DNP IgG1 antibody is not diminished.

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