Chitosan-carbon Nanofiber Modified Single-use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

Abstract DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

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Nanoparticle-based electrochemical detection of hepatitis B virus using stripping chronopotentiometry

Analytical Biochemistry ◽

10.1016/j.ab.2007.06.029 ◽

2007 ◽

Vol 370 (2) ◽

pp. 195-200 ◽

Cited By ~ 26

Author(s):

H. Hanaee ◽

H. Ghourchian ◽

A-A. Ziaee

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Electrochemical Detection ◽

Stripping Chronopotentiometry ◽

B Virus

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Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes

Blood ◽

10.1182/blood.v66.6.1251.bloodjournal6661251 ◽

1985 ◽

Vol 66 (6) ◽

pp. 1251-1253

Author(s):

DI Hoar ◽

T Bowen ◽

D Matheson ◽

MC Poon

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Dna Sequences ◽

Polymorphonuclear Leukocyte ◽

Dna Hybridization ◽

Unit Length ◽

Viral Dna ◽

B Genome ◽

B Virus ◽

Free Monomer

DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

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Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE

Bioelectrochemistry ◽

10.1016/j.bioelechem.2006.09.002 ◽

2007 ◽

Vol 70 (2) ◽

pp. 245-249 ◽

Cited By ~ 46

Author(s):

Guo Mandong ◽

Li Yanqing ◽

Guo Hongxia ◽

Wu Xiaoqin ◽

Fan Lifang

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Electrochemical Detection ◽

B Virus

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Determination of hepatitis B virus surface antigen in serum with a sandwich immunoassay and capillary electrophoresis–electrochemical detection

Analytical Methods ◽

10.1039/c4ay00109e ◽

2014 ◽

Vol 6 (8) ◽

pp. 2484-2489 ◽

Cited By ~ 7

Author(s):

Yu Chen ◽

Jie Wang ◽

Zhongming Liu ◽

Guoquan Wu

Keyword(s):

Capillary Electrophoresis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Electrochemical Detection ◽

Surface Antigen ◽

Virus Surface ◽

Virus Surface Antigen ◽

Detection Technology ◽

B Virus

We present a novel immunoassay that uses an efficient and specific sandwich-type protocol and sensitive capillary electrophoresis–electrochemical detection technology for the determination of hepatitis B virus surface antigen.

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