scholarly journals Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1251-1253
Author(s):  
DI Hoar ◽  
T Bowen ◽  
D Matheson ◽  
MC Poon
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Polymorphonuclear Leukocyte ◽  
Dna Hybridization ◽  
Unit Length ◽  
Viral Dna ◽  
B Genome ◽  
B Virus ◽  
Free Monomer

DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

scholarly journals Hepatitis B virus DNA is enriched in polymorphonuclear leukocytes

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1251-1253 ◽  
Author(s):  
DI Hoar ◽  
T Bowen ◽  
D Matheson ◽  
MC Poon
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Polymorphonuclear Leukocyte ◽  
Dna Hybridization ◽  
Unit Length ◽  
Viral Dna ◽  
B Genome ◽  
B Virus ◽  
Free Monomer

Abstract DNA hybridization and cell separation techniques were used to determine which blood components contained hepatitis B viral DNA sequences. Free monomer-length hepatitis B virus was found in large amounts in the polymorphonuclear leukocyte cell fraction in two of five HBsAG-positive patients. In these two patients, viral DNA sequences were not detected in the plasma or platelet fraction, whereas the mononuclear cell DNA contained small amounts of a 7.2 kb size unintegrated hepatitis B genome. These studies indicate that the major reservoir of unit-length viral DNA in the asymptomatic hepatitis B carriers studied here was in the polymorphonuclear leukocyte fraction. The basis for the presence of the viral DNA within these cells is presently unknown, but may relate to viral replication within, or phagocytosis of virus by, these cells.

scholarly journals Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 753
Author(s):  
Ece Yaralı ◽  
Arzum Erdem
Keyword(s):  
Ionic Liquid ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Phosphate Buffer Solution ◽  
Cobalt Phthalocyanine ◽  
Voltammetric Detection ◽  
B Virus

In this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning electron microscopy (SEM), and the electrochemical behavior of electrodes were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The voltammetric detection of hybridization was investigated by evaluating the guanine oxidation signal, measured by differential pulse voltammetry (DPV) technique. The implementation of our biosensor to serum samples was also examined using fetal bovine serum (FBS). The detection limit was established as 0.19 µg/mL in phosphate buffer solution (PBS) (pH 7.40) and 2.48 µg/mL in FBS medium. The selectivity of our assay regarding HBV DNA hybridization in FBS medium was tested in the presence of other DNA sequences. With this aim, the hybridization of DNA probe with non-complementary (NC) or mismatched DNA sequence (MM), or in the presence of mixture samples containing DNA target NC (1:1) or DNA target MM (1:1), was studied based on the changes in guanine signal.

scholarly journals Detection of hepatitis B virus DNA sequences in bone marrow of children with leukemia

Cancer ◽  
1987 ◽  
Vol 59 (2) ◽  
pp. 292-296 ◽  
Author(s):  
Patrizia Pontisso ◽  
Anna Locasciulli ◽  
Emma Schiavon ◽  
Giorgio Cattoretti ◽  
Raffaella Schirò ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Sequences ◽  
Hepatitis B Virus Dna ◽  
B Virus

scholarly journals Hepatitis B Virus Replication Induces Methylation of both Host and Viral DNA

2010 ◽  
Vol 84 (9) ◽  
pp. 4321-4329 ◽  
Author(s):  
Perumal Vivekanandan ◽  
Hubert Darius-J Daniel ◽  
Rajesh Kannangai ◽  
Francisco Martinez-Murillo ◽  
Michael Torbenson
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Replication ◽  
Cell Lines ◽  
Natural Infection ◽  
Cpg Islands ◽  
Hbv Infection ◽  
Viral Dna ◽  
B Virus

ABSTRACT Control of viral replication is a major therapeutic goal to reduce morbidity and mortality from chronic hepatitis B virus (HBV) infection. Recently, methylation has been identified as a novel host defense mechanism, and methylation of viral DNA leads to downregulation of HBV gene expression. To better understand the mechanisms of HBV methylation, cell lines were exposed to HBV using a model system that mimics natural infection and the expression of host DNA methyltransferase genes (DNMTs) was measured. DNMT1, DNMT2, and DNMT3 were all significantly upregulated in response to HBV. DNMT3 was further studied because of its known role in the de novo methylation of DNA. Cotransfection experiments with full-length HBV and DNMT3 led to the downregulation of viral protein and pregenomic RNA production. To investigate whether the upregulation of DNMTs could also have an effect on the methylation of host DNA, cell lines were exposed to HBV in two independent model systems, one that mimics natural infection and a second model with temporary transfection. Host DNA methylation was measured by DNA microarray analysis. Increased methylation of host CpG islands was detected in both experimental systems. Two CpG islands, corresponding to genes SUFU and TIRAP, were selected, and the downregulation of these genes in hepatocellular carcinomas was confirmed. In conclusion, hepatocytes respond to HBV infection by upregulating DNMTs. The DNMTs methylate viral DNA, leading to decreased viral gene expression and decreased viral replication. However, virus-induced overexpression of DNMTs also leads to methylation of host CpG islands.

scholarly journals Dominant negative mutants of the duck hepatitis B virus core protein interfere with RNA pregenome packaging and viral DNA synthesis

Hepatology ◽  
1999 ◽  
Vol 30 (1) ◽  
pp. 308-315 ◽  
Author(s):  
Fritz von Weizsäcker ◽  
Josef Köck ◽  
Stefan Wieland ◽  
Wolf-Bernhard Offensperger ◽  
Hubert E. Blum
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Synthesis ◽  
Core Protein ◽  
Dominant Negative ◽  
Viral Dna ◽  
Duck Hepatitis B Virus ◽  
Virus Core ◽  
Duck Hepatitis ◽  
B Virus
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