scholarly journals Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance

2008 ◽  
pp. NA-NA ◽  
Author(s):  
Ming-Wei Li ◽  
Rui-Qing Lin ◽  
Hui-Qun Song ◽  
Rehana A. Sani ◽  
Xiang-Yun Wu ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Animal Health ◽  
Electrophoretic Analysis ◽  
Sequence Variability ◽  
Health Significance

Sequence variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana of human and animal health significance

2011 ◽  
Vol 86 (3) ◽  
pp. 271-275 ◽  
Author(s):  
W. Liu ◽  
G.H. Liu ◽  
F. Li ◽  
D.S. He ◽  
T. Wang ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Nadh Dehydrogenase ◽  
Animal Health ◽  
Hunan Province ◽  
Sequence Variability ◽  
Specific Sequence ◽  
Sequence Variations ◽  
Geographical Regions ◽  
Health Significance ◽  
Polymerase Chain

AbstractSequence variability in three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 3 (cox3), NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) in Spirometra erinaceieuropaei spargana from different geographical regions in China was examined. A portion of each of the cox3 (pcox3), nad1 (pnad1) and nad4 genes (pnad4) were amplified separately from individual S. erinaceieuropaei spargana by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. The sequences of pcox3, pnad1 and pnad4 were 541, 607 and 847 bp in length, respectively. The A+T contents of the sequences were 68.39–68.76% (pcox3), 63.76–64.91% (pnad1) and 67.18–67.77% (pnad4), respectively, while the intra-specific sequence variations within each of the S. erinaceieuropaei spargana were 0–1.5% for pcox3, 0–2.8% for pnad1 and 0–2.7% for pnad4. Phylogenetic analysis using neighbour joining (NJ), maximum likelihood (ML) and maximum parsimony (MP) methods, indicated that all the spargana isolates in Hunan Province represented S. erinaceieuropaei. These findings demonstrated clearly the usefulness of the three mtDNA sequences for population genetics studies of S. erinaceieuropaei spargana of human and animal health significance.

Cryptosporidium species in post-weaned and adult sheep and goats from N.W. Spain: Public and animal health significance

2018 ◽  
Vol 254 ◽  
pp. 1-5 ◽  
Author(s):  
P. Díaz ◽  
E. Navarro ◽  
A. Prieto ◽  
A. Pérez-Creo ◽  
M. Viña ◽  
...  
Keyword(s):  
Animal Health ◽  
Cryptosporidium Species ◽  
Sheep And Goats ◽  
Adult Sheep ◽  
Health Significance

scholarly journals Interspecific mitochondrial DNA transfer and the colonization of Scandinavia by mice

1987 ◽  
Vol 49 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Ulf Gyllensten ◽  
Allan C. Wilson
Keyword(s):  
Mitochondrial Dna ◽  
Hybrid Zone ◽  
Restriction Enzymes ◽  
Electrophoretic Analysis ◽  
Nuclear Genes ◽  
Mouse Population ◽  
Apparent Lack ◽  
North Germany ◽  
Founder Event ◽  
The Baltic

SummaryRestriction enzymes were used to search for genetic variability at 162 cleavage sites in mitochondrial DNA (mtDNA) purified from 22 mice caught at seven Swedish localities. Although all of these mice bear the nuclear genes of Mus musculus, they bear the mtDNA of M. domesticus exclusively. Yet, some of the Swedish localities are 750 km away from the hybrid zone between these two species. Furthermore, only one type of mtDNA was found at the seven Swedish localities; this type was found before at an eighth locality in Sweden as well as in Jutland north of the hybrid zone. The apparent lack of mtDNA divergence in the mouse population of Sweden contrasts with the extensive divergence usually found within other geographic areas in Europe, Africa and North America. Electrophoretic analysis of proteins encoded by nuclear genes indicates that the Swedish mice have lower average heterozygosity than Danish and Central European populations of musculus mice. These findings lead us to suggest that the source of the commensal mouse population in Sweden was a small propagule that originated from a population situated only a few kilometres to the east of the point at which the hybrid zone on the European mainland meets the Baltic Sea, namely on East Holstein. Such a founder event may have been associated with the spread of farming from north Germany into Sweden about 4000 years ago.

Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China

2011 ◽  
Vol 86 (2) ◽  
pp. 245-251 ◽  
Author(s):  
R.S. Dai ◽  
G.H. Liu ◽  
H.Q. Song ◽  
R.Q. Lin ◽  
Z.G. Yuan ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
The Other ◽  
Sequence Variability ◽  
Taenia Hydatigena ◽  
Specific Sequence ◽  
5.8S Rdna ◽  
Rdna Sequences

AbstractSequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochromecoxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4), and internal transcribed spacer (ITS) of rDNA among and within three cestodes,Spirometra erinaceieuropaei,Taenia multicepsandTaenia hydatigena, from different geographical origins in China was examined. A portion of thecox1 (pcox1),nad4 genes (pnad4) and the ITS (ITS1+5.8S rDNA+ITS2) were amplified separately from individual cestodes by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. While the intra-specific sequence variations within each of the tapeworm species were 0–0.7% for pcox1, 0–1.7% for pnad4 and 0.1–3.6% for ITS, the inter-specific sequence differences were significantly higher, being 12.1–17.6%, 18.7–26.2% and 31–75.5% for pcox1, pnad4 and ITS, respectively. Phylogenetic analyses based on the pcox1 sequence data revealed thatT. multicepsandT. hydatigenawere more closely related to the other members of theTaeniagenus, andS. erinaceieuropaeiwas more closely related to the other members of theSpirometragenus. These findings demonstrated clearly the usefulness of mtDNA and rDNA sequences for population genetic studies of these cestodes of socio-economic importance.

scholarly journals Molecular Survey of Viral Poultry Diseases with an Indirect Public Health Significance in Central Ethiopia

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3564
Author(s):  
Behailu Assefa Wayou ◽  
Gezahegne Mamo Kassa ◽  
Daniela Pasotto ◽  
Teshale Sori ◽  
Claudia Maria Tucciarone ◽  
...  
Keyword(s):  
Disease Virus ◽  
Animal Health ◽  
Food Resource ◽  
Bursa Of Fabricius ◽  
Poultry Production ◽  
Fta Cards ◽  
High Quality Protein ◽  
Bursal Disease ◽  
Routine Basis ◽  
Health Significance

The importance of poultry production is globally increasing, in Ethiopia as well, where high-quality protein and contained costs make poultry a valuable food resource. However, this entails some problems linked to rural, backyard and intensively reared flock proximity and pathogen circulation. This study is aimed at monitoring the presence of important viral pathogens in poultry (infectious bronchitis virus (IBV), avian metapneumovirus (aMPV), infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV)) in Ethiopia. Respiratory and cloacal swabs and bursa of Fabricius and kidney imprints on FTA cards were collected in 2021 from 16 farms and tested for IBV, aMPV, NDV and IBDV. One farm was positive for IBDV, resulting in strains similar to those present in vaccines, belonging to genogroup A1a; two farms were positive for IBV but, due to sensitivity limits, only one sample was sequenced, resulting in a 4/91-like strain (GI-13); a layer farm tested positive for NDV with a Lasota-like vaccine strain. These findings suggest a low presence of these pathogens, probably due to the implementation of vaccination strategies, which is also testified by the detection of vaccine strains. A close diagnostic activity should be implemented on a routine basis in order to monitor pathogen circulation, ameliorate biosecurity measures and protect animal health and production levels.

scholarly journals The complete mitochondrial genomes for three Toxocara species of human and animal health significance

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 224 ◽  
Author(s):  
Ming-Wei Li ◽  
Rui-Qing Lin ◽  
Hui-Qun Song ◽  
Xiang-Yun Wu ◽  
Xing-Quan Zhu
Keyword(s):  
Animal Health ◽  
Mitochondrial Genomes ◽  
Health Significance ◽  
Toxocara Species ◽  
Complete Mitochondrial Genomes

scholarly journals Characterization of MicroRNAs from Orientobilharzia turkestanicum, a Neglected Blood Fluke of Human and Animal Health Significance

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47001 ◽  
Author(s):  
Chun-Ren Wang ◽  
Min-Jun Xu ◽  
Jing-Hua Fu ◽  
Alasdair J. Nisbet ◽  
Qiao-Cheng Chang ◽  
...  
Keyword(s):  
Animal Health ◽  
Blood Fluke ◽  
Health Significance

scholarly journals Enhanced diagnosis of rabies and molecular evidence for the transboundary spread of the disease in Mozambique

2017 ◽  
Vol 88 ◽  
Author(s):  
Andre Coetzer ◽  
Iolanda Anahory ◽  
Paula T. Dias ◽  
Claude T. Sabeta ◽  
Terence P. Scott ◽  
...  
Keyword(s):  
Current Knowledge ◽  
Phylogenetic Analyses ◽  
Fluorescent Antibody ◽  
Animal Health ◽  
Molecular Evidence ◽  
Reference Laboratory ◽  
Diagnostic Laboratory ◽  
Negative Results ◽  
Health Significance ◽  
Diagnostic Capabilities

Rabies is a neglected zoonotic disease with veterinary and public health significance, particularly in Africa and Asia. The current knowledge of the epidemiology of rabies in Mozambique is limited because of inadequate sample submission, constrained diagnostic capabilities and a lack of molecular epidemiological research. We wanted to consider the direct, rapid immunohistochemical test (DRIT) as an alternative to the direct fluorescent antibody (DFA) for rabies diagnosis at the diagnostic laboratory of the Central Veterinary Laboratory (CVL), Directorate of Animal Science, Maputo, Mozambique. Towards this aim, as a training exercise at the World Organisation for Animal Health (OIE) Rabies Reference Laboratory in South Africa, we performed the DRIT on 29 rabies samples from across Mozambique. With the use of the DRIT, we found 15 of the 29 samples (52%) to be negative. The DRIT-negative samples were retested by DFA at the OIE Rabies Reference Laboratory, as well as with an established real-time Polymerase chain reaction, confirming the DRIT-negative results. The DRIT-positive results (14/29) were retested with the DFA and subsequently amplified, sequenced and subjected to phylogenetic analyses, confirming the presence of rabies RNA. Molecular epidemiological analyses that included viruses from neighbouring countries suggested that rabies cycles within Mozambique might be implicated in multiple instances of cross-border transmission. In this regard, our study has provided new insights that should be helpful in informing the next steps required to better diagnose, control and hopefully eliminate rabies in Mozambique.

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