Skull base cerebrospinal fluid fistula: a novel detection method based on two-dimensional electrophoresis

Head & Neck ◽  
2004 ◽  
Vol 26 (5) ◽  
pp. 464-469 ◽  
Author(s):  
Alma Ricchetti ◽  
Pierre R. Burkhard ◽  
Neftali Rodrigo ◽  
Zdravko Gamulin ◽  
Mélanie Côte ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Skull Base ◽  
Detection Method ◽  
Two Dimensional ◽  
Cerebrospinal Fluid Fistula ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Fluid Fistula

Extradural cartilage inlay graft in cerebrospinal fluid fistula repair

2010 ◽  
Vol 124 (12) ◽  
pp. 1294-1297 ◽  
Author(s):  
P Thulasi Das ◽  
D Balasubramanian
Keyword(s):  
Cerebrospinal Fluid ◽  
Skull Base ◽  
Success Rate ◽  
Bony Defect ◽  
Cerebrospinal Fluid Fistula ◽  
Fistula Repair ◽  
Cerebrospinal Fluid Rhinorrhoea ◽  
Skull Base Defects ◽  
Fluid Fistula

AbstractObjective:To present our experience in managing cerebrospinal fluid rhinorrhoea using the cartilage inlay (underlay) technique to repair skull base defects larger than 4 mm.Study design:Retrospective study involving patients presenting with cerebrospinal fluid rhinorrhoea between 1994 and 2008.Setting:Patients were treated in a tertiary referral centre for nose and sinus diseases. Patients' medical records were reviewed and analysed.Results:A total of 62 patients were operated upon using a cartilage inlay technique to repair bony skull base defects ranging in size from 4 to 20 mm (widest diameter). Of these 62 patients, 16 constituted revisions of earlier procedures undertaken elsewhere. The success rate of the technique was 100 per cent. Patient follow up ranged from six months to 16 years, with a median follow up of 15 months. Three patients had minor post-operative sinus infections; no serious complications were encountered.Conclusion:Extradural cartilage inlay appears to be an effective technique in the management of cerebrospinal fluid rhinorrhoea, especially for large defects and revision procedures. To our knowledge, the described patients represent the largest reported series of cerebrospinal fluid rhinorrhoea cases managed using the cartilage inlay technique. We believe that the crucial factors in our high success rate for cerebrospinal fluid fistula repair are: precise identification of the bony defect; meticulous preparation of the graft bed; careful elevation of the dura; judicious use of just enough graft tissue; and adequate graft support.

Enhancement techniques for detecting trace and fluid-specific components in two-dimensional electrophoresis patterns.

1982 ◽  
Vol 28 (4) ◽  
pp. 759-765 ◽  
Author(s):  
G B Dermer ◽  
L M Silverman ◽  
J F Chapman
Keyword(s):  
Cerebrospinal Fluid ◽  
Serum Proteins ◽  
Prostatic Acid Phosphatase ◽  
Two Dimensional ◽  
Cibacron Blue ◽  
Two Dimensional Electrophoresis ◽  
Prostatic Fluid ◽  
Creatine Kinase B ◽  
Malignant Effusions ◽  
Dimensional Electrophoresis

Abstract Albumin and other serum-derived proteins were removed from several types of body fluids by affinity chromatography, to facilitate detection of trace or non-serum-derived proteins in two-dimensional electrophoresis patterns. Albumin was removed by the dye Cibacron Blue F3G-A coupled to Sepharose. Two-dimensional patterns of albumin-depleted serum lack the large albumin spot, and several families of spots become visible that ordinarily are partly or totally hidden by it. However, other proteins also bind to Cibacron Blue. Most serum proteins, including albumin, were effectively removed by anti-human serum antibodies coupled to Sepharose. Two-dimensional patterns of serum-depleted cerebrospinal fluid exhibit five clusters of probable nervous-system protein families not detected in serum. One additional family, probably antigenically related to transferrin, was removed by the affinity step. Two-dimensional patterns of serum-depleted prostatic fluid exhibit five major non-serum families, two of which may be creatine kinase B subunits and prostatic acid phosphatase. Two-dimensional patterns of serum-depleted malignant effusions exhibit one or more of three proteins that possibly are tumor products. Pattern matching suggests the presence of one non-serum-derived protein family common to cerebrospinal fluid, prostatic fluid, and malignant effusions. Prostatic fluid and malignant effusions have in common as many as three non-serum families of proteins.

The use of platelet gel for repair of a cerebrospinal fluid fistula of the skull base (a case report and literature review)

2018 ◽  
Vol 82 (1) ◽  
pp. 86
Author(s):  
O. I. Sharipov ◽  
M. A. Kutin ◽  
A. V. Bayuklin ◽  
A. A. Imaev ◽  
A. A. Abdilatipov ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Case Report ◽  
Literature Review ◽  
Skull Base ◽  
Cerebrospinal Fluid Fistula ◽  
Platelet Gel ◽  
Fluid Fistula

Assessing cerebrospinal fluid rhinorrhea: A two-dimensional electrophoresis approach

2001 ◽  
Vol 22 (9) ◽  
pp. 1826-1833 ◽  
Author(s):  
Pierre R. Burkhard ◽  
Neftali Rodrigo ◽  
Daniel May ◽  
Roman Sztajzel ◽  
Jean-Charles Sanchez ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Two Dimensional ◽  
Cerebrospinal Fluid Rhinorrhea ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
Sign in / Sign up

Export Citation Format

Share Document