scholarly journals Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues

2001 ◽  
Vol 92 (6) ◽  
pp. 856-860 ◽  
Author(s):  
Achim A. Jungbluth ◽  
Yao-Tseng Chen ◽  
Elisabeth Stockert ◽  
Klaus J. Busam ◽  
Denise Kolb ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Human Tissues

Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues

2021 ◽  
pp. 27-42
Author(s):  
Davide Vecchiotti ◽  
Daniela Verzella ◽  
Daria Capece ◽  
Jessica Cornice ◽  
Mauro Di Vito Nolfi ◽  
...  
Keyword(s):  
Nuclear Translocation ◽  
Immunohistochemical Analysis ◽  
Human Tissues

Polypoid intranasal mass caused by Rosai–Dorfman disease: a diagnostic pitfall

2009 ◽  
Vol 124 (3) ◽  
pp. 345-348 ◽  
Author(s):  
M Ilie ◽  
N Guevara ◽  
L Castillo ◽  
P Hofman
Keyword(s):  
Correct Diagnosis ◽  
Disease Process ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Extranodal Involvement ◽  
Microscopic Appearance ◽  
Radiological Features ◽  
Diagnostic Pitfall ◽  
Rosai Dorfman Disease ◽  
Neoplastic Disorders

AbstractBackground:Rosai–Dorfman disease is a rare, idiopathic, histiocytic proliferative disorder with a distinctive microscopic appearance, which was formerly thought to be a disease process limited to lymph nodes. However, extranodal involvement has been documented in less than half of the reported patients, but rarely without associated lymphadenopathy.Case report:We report the case of a 43-year-old Senegalese woman who presented with a polypoid, intranasal mass caused by Rosai–Dorfman disease. A diagnosis of a granulomatous process, including rhinoscleroma, was initially discussed. The correct diagnosis was made histologically by demonstrating aggregates of histiocytes with large amounts of cytoplasm, emperipolesis and protein S100 antigen expression. Despite using ancillary methods (molecular biology and electron microscopy), we failed to demonstrate any associated pathogen.Conclusion:Diagnosis of Rosai–Dorfman disease can be very difficult, in particular in adults from Africa with pure, isolated, intranasal localisation, in whom clinical and radiological features may mimic other infectious or neoplastic disorders. The diagnosis is made based on the histological presence of large histiocytes with lymphophagocytosis. Moreover, immunohistochemical analysis of these histiocytes using anti-protein S100 antibody shows strong positivity.

scholarly journals The protein expression profile of ACE2 in human tissues

2020 ◽  
Author(s):  
Feria Hikmet ◽  
Loren Méar ◽  
Åsa Edvinsson ◽  
Patrick Micke ◽  
Mathias Uhlén ◽  
...  
Keyword(s):  
Host Cell ◽  
Immunohistochemical Analysis ◽  
Cell Types ◽  
Cell Entry ◽  
Cell Surface Receptor ◽  
Renal Tubules ◽  
Human Tissues ◽  
Specific Expression ◽  
Ductal Cells ◽  
Host Cell Entry

ABSTRACTThe novel SARS-coronavirus 2 (SARS-CoV-2) poses a global challenge on healthcare and society. For understanding the susceptibility for SARS-CoV-2 infection, the cell type-specific expression of the host cell surface receptor is necessary. The key protein suggested to be involved in host cell entry is Angiotensin I converting enzyme 2 (ACE2). Here, we report the expression pattern of ACE2 across >150 different cell types corresponding to all major human tissues and organs based on stringent immunohistochemical analysis. The results were compared with several datasets both on the mRNA and protein level. ACE2 expression was mainly observed in enterocytes, renal tubules, gallbladder, cardiomyocytes, male reproductive cells, placental trophoblasts, ductal cells, eye and vasculature. In the respiratory system, the expression was limited, with no or only low expression in a subset of cells in a few individuals, observed by one antibody only. Our data constitutes an important resource for further studies on SARS-CoV-2 host cell entry, in order to understand the biology of the disease and to aid in the development of effective treatments to the viral infection.

Molecular similarities between primary peritoneal and primary ovarian carcinomas

2003 ◽  
Vol 13 (6) ◽  
pp. 749-755 ◽  
Author(s):  
L.-M. Chen ◽  
S. D. Yamada ◽  
Y.-S. Fu ◽  
R. L. Baldwin ◽  
B. Y. Karlan
Keyword(s):  
Expression Patterns ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Metastatic Tumor ◽  
Peritoneal Carcinoma ◽  
Ovarian Carcinomas ◽  
Serous Ovarian Carcinoma ◽  
Biologic Markers ◽  
Her 2 ◽  
Tissue Site

The objective of this paper was to characterize expression patterns of biologic markers to distinguish papillary serous peritoneal carcinoma (PPC) from papillary serous ovarian carcinoma (POC). Immunohistochemical analysis of HER-2/neu, p53, bcl-2, and nm23-H1 expression was performed on archival paraffin-embedded tissues. Antigen expression was compared at ovarian and extra-ovarian sites. Thirty-two PPC cases were compared to 18 POC cases. Mean age, stage, grade, and survival outcome were comparable between the two groups. Antigen expression patterns were not significantly different between PPC and POC for the four markers studied. In all cases, nm23-H1 was expressed. Conversely, bcl-2 was expressed at only a single tissue site in three of 32 (9.4%) PPC cases and in one of 18 (5.6%) POC cases. Eleven of 32 (34.4%) PPC cases overexpressed HER-2/neu, vs. four of 18 (22.2%) POC cases. P53 staining results were positive in 23 of 32 (71.9%) PPC and 13 of 18 (72.2%) POC cases. Intrapatient antigen expression was identical at primary and metastatic tumor sites in 50% of the POC and 48.4% of the PPC cases. We conclude that PPC and POC have a comparable immunohistochemical phenotype for these four molecular markers, which is reflected by their similar clinical courses.

Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer

2003 ◽  
Vol 88 (3) ◽  
pp. 635-642 ◽  
Author(s):  
James B. DuHadaway ◽  
Frank J. Lynch ◽  
Shawn Brisbay ◽  
Carlos Bueso-Ramos ◽  
Patricia Troncoso ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Immunohistochemical Analysis ◽  
Human Tissues ◽  
Nuclear Expression

Immunohistochemical analysis of P185 and ASL activity in transformed human tissues

1997 ◽  
Vol 30 (3) ◽  
pp. 285
Author(s):  
L. Terzuoli ◽  
B. Frosi ◽  
B. Porcelli ◽  
F. Carlucci ◽  
C. Minacci ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Human Tissues

Immunohistochemical analysis of p186 in normal and trasformed human tissues

1996 ◽  
Vol 24 (2) ◽  
pp. 306S-306S
Author(s):  
PAGANI ROBERTO ◽  
PORCELLI BRUNETTA ◽  
FROSI BARBARA ◽  
PIZZICHINI MARIA ◽  
VERNILLO REMO ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Human Tissues

Immunohistochemical analysis of steroid sulfatase in human tissues

2007 ◽  
Vol 105 (1-5) ◽  
pp. 115-123 ◽  
Author(s):  
Kyle W. Selcer ◽  
Heidi M. DiFrancesca ◽  
Abhinav B. Chandra ◽  
Pui-Kai Li
Keyword(s):  
Immunohistochemical Analysis ◽  
Steroid Sulfatase ◽  
Human Tissues
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