scholarly journals Jungbluth AA, Chen Y-T, Stockert E., Busam KJ, Kolb D, Iversen K, Coplan K, Williamson B, Altorki N, Old LJ. Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues.International Journal of Cancer 2001; 92(6) 856-860.

2002 ◽  
Vol 97 (6) ◽  
pp. 878-878 ◽  
Author(s):  
AA Jungbluth ◽  
Y-T Chen ◽  
E Stockert ◽  
KJ Busam ◽  
D Kolb ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Antigen Expression

scholarly journals Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues

2001 ◽  
Vol 92 (6) ◽  
pp. 856-860 ◽  
Author(s):  
Achim A. Jungbluth ◽  
Yao-Tseng Chen ◽  
Elisabeth Stockert ◽  
Klaus J. Busam ◽  
Denise Kolb ◽  
...  
Keyword(s):  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Human Tissues

Polypoid intranasal mass caused by Rosai–Dorfman disease: a diagnostic pitfall

2009 ◽  
Vol 124 (3) ◽  
pp. 345-348 ◽  
Author(s):  
M Ilie ◽  
N Guevara ◽  
L Castillo ◽  
P Hofman
Keyword(s):  
Correct Diagnosis ◽  
Disease Process ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Extranodal Involvement ◽  
Microscopic Appearance ◽  
Radiological Features ◽  
Diagnostic Pitfall ◽  
Rosai Dorfman Disease ◽  
Neoplastic Disorders

AbstractBackground:Rosai–Dorfman disease is a rare, idiopathic, histiocytic proliferative disorder with a distinctive microscopic appearance, which was formerly thought to be a disease process limited to lymph nodes. However, extranodal involvement has been documented in less than half of the reported patients, but rarely without associated lymphadenopathy.Case report:We report the case of a 43-year-old Senegalese woman who presented with a polypoid, intranasal mass caused by Rosai–Dorfman disease. A diagnosis of a granulomatous process, including rhinoscleroma, was initially discussed. The correct diagnosis was made histologically by demonstrating aggregates of histiocytes with large amounts of cytoplasm, emperipolesis and protein S100 antigen expression. Despite using ancillary methods (molecular biology and electron microscopy), we failed to demonstrate any associated pathogen.Conclusion:Diagnosis of Rosai–Dorfman disease can be very difficult, in particular in adults from Africa with pure, isolated, intranasal localisation, in whom clinical and radiological features may mimic other infectious or neoplastic disorders. The diagnosis is made based on the histological presence of large histiocytes with lymphophagocytosis. Moreover, immunohistochemical analysis of these histiocytes using anti-protein S100 antibody shows strong positivity.

Molecular similarities between primary peritoneal and primary ovarian carcinomas

2003 ◽  
Vol 13 (6) ◽  
pp. 749-755 ◽  
Author(s):  
L.-M. Chen ◽  
S. D. Yamada ◽  
Y.-S. Fu ◽  
R. L. Baldwin ◽  
B. Y. Karlan
Keyword(s):  
Expression Patterns ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Metastatic Tumor ◽  
Peritoneal Carcinoma ◽  
Ovarian Carcinomas ◽  
Serous Ovarian Carcinoma ◽  
Biologic Markers ◽  
Her 2 ◽  
Tissue Site

The objective of this paper was to characterize expression patterns of biologic markers to distinguish papillary serous peritoneal carcinoma (PPC) from papillary serous ovarian carcinoma (POC). Immunohistochemical analysis of HER-2/neu, p53, bcl-2, and nm23-H1 expression was performed on archival paraffin-embedded tissues. Antigen expression was compared at ovarian and extra-ovarian sites. Thirty-two PPC cases were compared to 18 POC cases. Mean age, stage, grade, and survival outcome were comparable between the two groups. Antigen expression patterns were not significantly different between PPC and POC for the four markers studied. In all cases, nm23-H1 was expressed. Conversely, bcl-2 was expressed at only a single tissue site in three of 32 (9.4%) PPC cases and in one of 18 (5.6%) POC cases. Eleven of 32 (34.4%) PPC cases overexpressed HER-2/neu, vs. four of 18 (22.2%) POC cases. P53 staining results were positive in 23 of 32 (71.9%) PPC and 13 of 18 (72.2%) POC cases. Intrapatient antigen expression was identical at primary and metastatic tumor sites in 50% of the POC and 48.4% of the PPC cases. We conclude that PPC and POC have a comparable immunohistochemical phenotype for these four molecular markers, which is reflected by their similar clinical courses.

Comparative Study of Tamoxifen and Raloxifene on Endometrial Cell Proliferation of Female Rats in Persistent Estrus

2012 ◽  
Vol 22 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Dorival Mendes Rodrigues Junior ◽  
Alesse Ribeiro dos Santos ◽  
Pedro Vitor Lopes Costa ◽  
Benedito Borges da Silva
Keyword(s):  
Protein Expression ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Female Rats ◽  
Ki 67 ◽  
Subcutaneous Dose ◽  
Persistent Estrus ◽  
Group 3 ◽  
Group 2 ◽  
Endometrial Epithelium

ObjectiveThe objective of the study was to compare the effect of tamoxifen and raloxifene on the endometrium of female rats in persistent estrus, by Ki-67 protein expression.MethodsThe study comprised 60 Wistar-Hannover female rats in persistent estrus, induced by a single subcutaneous dose of 1.25 mg of testosterone propionate on the second day of age. At 90 days of life, the animals were randomly divided into 3 groups of 20 animals each. Group 1 (control), received only placebo; group 2, the animals were treated with tamoxifen, 250 μg/d; and group 3, the rats were treated with 750 μg/d of raloxifene by gavage during 30 days. Then, the animals were killed, and the endometrium was removed for immunohistochemical analysis of Ki-67 antigen expression. Statistical analysis was performed by β regression model (P < 0.05).ResultsMean percentages of Ki-67 protein expression in the endometrium of rats in persistent estrus were 43.21% ± 3.39%, 7.36% ± 0.95%, and 7.20% ± 0.76% in groups 1, 2 and 3, respectively (P < 0.001). There was no statistical difference between groups 2 and 3 (P = 0.7159).ConclusionsThe present results indicate that, at the doses and during the time of treatment used, both tamoxifen and raloxifene induce atrophy in a similar way of endometrial epithelium of rats in persistent estrus.

scholarly journals Viral gene expression during acute simian varicella virus infection

2002 ◽  
Vol 83 (4) ◽  
pp. 841-846 ◽  
Author(s):  
Wayne L. Gray ◽  
Lisa Mullis ◽  
Kenneth F. Soike
Keyword(s):  
Gene Expression ◽  
Viral Antigen ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Pcr Analysis ◽  
Viral Gene ◽  
Vervet Monkeys ◽  
Simian Varicella Virus ◽  
Varicella Zoster ◽  
Varicella Virus

Simian varicella virus (SVV) causes a natural varicella-like disease in nonhuman primates. Outbreaks of simian varicella occur sporadically in primate facilities. Simian varicella is used as a model for investigation of varicella-zoster virus (VZV) pathogenesis and latency. In this study, SVV gene expression and histopathology were analysed in tissues of acutely infected vervet monkeys. RT–PCR analysis demonstrated expression of specific SVV immediate early, early and late genes in the skin, lung, liver and ganglia tissues of acutely infected monkeys. Viral antigen expression and histopathology, including necrosis and inflammation, were detected in the skin, lungs, liver and spleen of infected monkeys by immunohistochemical analysis. Viral antigen expression, but little or no histopathology, was evident in the neural ganglia, the eventual site of viral latency. The study provides a foundation for further investigation on the role of viral genes in varicella pathogenesis and latency.

Production of interferon-γ by microglia

2006 ◽  
Vol 12 (5) ◽  
pp. 558-564 ◽  
Author(s):  
J Kawanokuchi ◽  
T Mizuno ◽  
H Takeuchi ◽  
H Kato ◽  
J Wang ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
Immune Cell ◽  
Critical Role ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Interferon Γ ◽  
Lymphoid Cells ◽  
Neural Cells ◽  
Ifn Γ ◽  
Mhc Antigen

Neural cells do not usually interact with immune cells because of the lack of major histocompatibility complex (MHC) antigen expression. Interferon-γ (IFN-γ) enables this interaction via induction of MHC antigen expression in neural cells. Thus, IFN-γ is a critical cytokine for the development of central nervous system (CNS) pathologies. IFN-γ, however, is considered to be produced exclusively by lymphoid cells. Here, we show for the first time that murine microglia produce IFN-γ in response to IL-12 and/or IL-18, using RT-PCR detection of IFN-γ mRNA and Western blotting and immunohistochemical analysis for cytoplasmic expression of IFN-γ. Stimulation of microglia with IL-12 and IL-18 resulted in MHC class II mRNA expression in microglia. Since IL-12 and IL-18 are produced in the CNS by glial cells, these cytokines may play a critical role in the initiation of neural immune cell interaction and the induction of autoimmune processes in the CNS via induction of IFN-γ and MHC antigens.

Undifferentiated Tumor: True Identity by Immunohistochemistry

2008 ◽  
Vol 132 (3) ◽  
pp. 326-348 ◽  
Author(s):  
Armita Bahrami ◽  
Luan D. Truong ◽  
Jae Y. Ro
Keyword(s):  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Round Cell ◽  
Pertinent Literature ◽  
Small Round Cell ◽  
Poorly Differentiated ◽  
Undifferentiated Tumors ◽  
Microscopic Morphology ◽  
Round Cell Tumors

Abstract Context.—“Undifferentiated tumor” refers to a heterogeneous group of neoplasms with little or no evidence of differentiation on routine light microscopic morphology. Objective.—To identify the true identity of undifferentiated tumors by immunohistochemical analysis. Data Sources.—Review of the pertinent literature and the authors' experience. Conclusions.—For treatment and prognostic evaluation, it is crucial to delineate whether an undifferentiated neoplasm is epithelial, mesenchymal, melanocytic, or hematopoietic in nature. Application of a screening panel to demonstrate the expression of markers of major lineages is fundamental for determination of the broad category of neoplasia. Because poorly differentiated carcinomas and in particular sarcomatoid carcinomas are known to be heterogeneous in their antigen expression, several epithelial markers in combination may be required to establish the carcinomatous nature of tumor. A diagnostic misinterpretation as a consequence of occasional aberrant or unexpected antigen expression is best avoided by using a broad panel that includes both antibodies that are anticipated to be positive and those that are expected to be negative. In this treatise, the immunohistochemical dissection of undifferentiated tumors on the basis of their morphologic features is outlined, supplemented with algorithmic immunohistochemical analysis for each morphologic category of small round cell tumors, carcinomatous tumors, sarcomatous (or sarcoma-like) tumors, and tumors with histologically overlapping features, including hematolymphoid malignancies, melanoma, and sarcomas with epithelioid appearance. The utility of several organ- or tissue-specific markers in the context of undifferentiated tumors is reviewed.

Immunohistochemical analysis of developmental neural antigen expression in the balloon cells of focal cortical dysplasia

2011 ◽  
Vol 18 (1) ◽  
pp. 114-118 ◽  
Author(s):  
Chang-Woo Han ◽  
Byung-Woo Min ◽  
Young Kim ◽  
Eun-Hui Jeong ◽  
Chang-Soo Park ◽  
...  
Keyword(s):  
Focal Cortical Dysplasia ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Cortical Dysplasia ◽  
Balloon Cells

scholarly journals Immunohistochemical analysis of CDw52 antigen expression in non-Hodgkin's lymphomas.

1994 ◽  
Vol 47 (4) ◽  
pp. 313-317 ◽  
Author(s):  
J R Salisbury ◽  
N T Rapson ◽  
J D Codd ◽  
M V Rogers ◽  
A B Nethersell
Keyword(s):  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Hodgkin's Lymphomas
Sign in / Sign up

Export Citation Format

Share Document