Establishment and characterization of two epithelial tumor cell lines (hne-1 and hone-1) latently infected with epstein-barr virus and derived from nasopharyngeal carcinomas

1990 ◽  
Vol 45 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Kaitai Yao ◽  
Hai-Ying Zhang ◽  
He-Cheng Zhu ◽  
Fu-Xi Wang ◽  
Gui-Yuan Li ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Tumor Cell Lines ◽  
Epithelial Tumor ◽  
Barr Virus ◽  
Epithelial Tumor Cell ◽  
Latently Infected ◽  
Epstein Barr

scholarly journals Identification of Epstein-Barr Virus RK-BARF0-Interacting Proteins and Characterization of Expression Pattern

2004 ◽  
Vol 78 (23) ◽  
pp. 12848-12856 ◽  
Author(s):  
Natalie J. Thornburg ◽  
Shuichi Kusano ◽  
Nancy Raab-Traub
Keyword(s):  
Cell Lines ◽  
Epstein Barr Virus ◽  
Interacting Proteins ◽  
Cellular Functions ◽  
Barr Virus ◽  
Acid Protein ◽  
Infected Cells ◽  
Epstein Barr ◽  
Endoplasmic Reticulum Targeting

ABSTRACT The Epstein-Barr virus (EBV) BamHI A transcripts are a family of transcripts that are differentially spliced and can be detected in multiple EBV-associated malignancies. Several of the transcripts may encode proteins. One transcript of interest, RK-BARF0, is proposed to encode a 279-amino-acid protein with a possible endoplasmic reticulum-targeting sequence. In this study, the properties of RK-BARF0 were examined through identification of cellular-interacting proteins through yeast two-hybrid analysis and characterization of its expression in EBV-infected cells and tumors. In addition to the interaction previously identified with cellular Notch, it was determined that RK-BARF0 also bound cellular human I-mfa domain-containing protein (HIC), epithelin, and scramblase. An interaction between RK-BARF0 and Notch or epithelin induced proteasome-dependent degradation of Notch and epithelin but not of HIC or scramblase. Low levels of endogenous Notch expression in EBV-positive cell lines may correlate with RK-BARF0 expression. However, a screen of EBV-positive cell lines and tumors with an affinity-purified α-RK-BARF0 antiserum did not consistently detect RK-BARF0. These data suggest that while RK-BARF0 may have important cellular functions during EBV infection, and while the phenotype of EBV-positive cells suggest its expression, RK-BARF0 levels may be too low to detect.

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