Characterization of a species of non-specific cross-reacting antigen (NCA) exressed by human monocytic cell lines: Structure and expression during cell differentiation

1994 ◽  
Vol 56 (6) ◽  
pp. 886-891 ◽  
Author(s):  
Motomu Kuroki ◽  
Takahiko Yamanaka ◽  
Yoshino Matsuo ◽  
Takeshi Ohtani ◽  
Yoshinobu Matsuo ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Cell Lines ◽  
Monocytic Cell ◽  
Human Monocytic Cell

Characterization of FcεRI Expressing Human Monocytic Cell Lines

1997 ◽  
Vol 113 (4) ◽  
pp. 444-453 ◽  
Author(s):  
I.G. Reischl ◽  
N. Corvaïa ◽  
J. Unger ◽  
M. Woisetschläger ◽  
G.C. Mudde
Keyword(s):  
Cell Lines ◽  
Monocytic Cell ◽  
Human Monocytic Cell

scholarly journals Factors affecting SP-A-mediated phagocytosis in human monocytic cell lines

2004 ◽  
Vol 98 (7) ◽  
pp. 637-650 ◽  
Author(s):  
Jianqiang Ding ◽  
Todd M Umstead ◽  
Joanna Floros ◽  
David S Phelps
Keyword(s):  
Cell Lines ◽  
Monocytic Cell ◽  
Factors Affecting ◽  
Human Monocytic Cell

Characterization of two novel pre-B-cell lines (LK63 and LiLa-1): Potential models of pre-B-cell differentiation

1992 ◽  
Vol 16 (6-7) ◽  
pp. 655-663 ◽  
Author(s):  
Evelyn Salvaris ◽  
Jürgen R. Novotny ◽  
Karen Welch ◽  
Lynda Campbell ◽  
Andrew W. Boyd
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
Cell Lines ◽  
B Cell Differentiation ◽  
Potential Models

scholarly journals Human Monocytic Cell Lines Transformed In Vitro by Epstein-Barr Virus Display a Type II Latency and LMP-1-Dependent Proliferation

2002 ◽  
Vol 76 (13) ◽  
pp. 6460-6472 ◽  
Author(s):  
Eric Masy ◽  
Eric Adriaenssens ◽  
Claire Montpellier ◽  
Pascale Crépieux ◽  
Alexandra Mougel ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Type Ii ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Barr Virus ◽  
Human Monocytic Cell ◽  
Epstein Barr ◽  
Monocytic Lineage

ABSTRACT Epstein-Barr virus (EBV) classically infects and transforms B lymphocytes in vitro, yielding lymphoblastoid cell lines (LCLs). In contrast to other herpesviruses, EBV is not described as an infectious agent for monocytes. However, recent papers described in vitro infection of monocytes leading to abortive or transient viral expression. In the present study, we report the characterization of E1, a monocytic cell line infected and transformed by EBV. This cell line was derived from an LCL by a drastic electroporation and selection of neomycin-resistant cells, unfavorable to B-cell outgrowth. E1 expressed surface molecules of monocytic lineage (CD14, major histocompatibility complex class II, and CD80) and the c-fms gene, a highly specific marker for the monocytic lineage. This cell line is able to phagocytose and secrete proinflammatory monokines tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8. E1 cells are tumorigenic after injection in nude mice, and a monocytic cell line obtained from one of these tumors (TE1) displayed immunophenotype and functional properties similar to those of E1. We detected the presence of the EBV genome in both cell lines, as well as expression of the EBNA-1 and LMP-1, but not EBNA-2, viral genes, characteristic of a type II latency. LMP-1 influences the phenotype of these monocytic cell lines, as demonstrated by down-regulation of cell proliferation and membrane intercellular adhesion molecule 1 expression due to an LMP-1 antisense strategy. This is the first description of a latently infected human monocytic cell line and the first direct demonstration of an instrumental role for LMP-1 in the proliferation of EBV-transformed cell lines expressing a type II latency.

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