scholarly journals Amino acid sequence of the ‘pathogenesis-related’ leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins

1985 ◽  
Vol 4 (11) ◽  
pp. 2745-2749 ◽  
Author(s):  
J. Lucas ◽  
A. Camacho Henriquez ◽  
F. Lottspeich ◽  
A. Henschen ◽  
H.L. Sänger
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Leaf Protein ◽  
Pathogenesis Related ◽  
Infected Tomato ◽  
New Type

Pathogenesis-Related Proteins in Trifolium subterraneum: A General Survey and Subsequent Characterisation of a Protein Inducible by Ethephon and Redlegged Earth Mite Attack

1997 ◽  
Vol 24 (6) ◽  
pp. 819 ◽  
Author(s):  
Kevin Broderick ◽  
Christopher Pittock ◽  
Tony Arioli ◽  
Ernest H. Creaser ◽  
Jeremy J. Weinman ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Chitinase Activity ◽  
Pr Proteins ◽  
Gene Encoding ◽  
Pathogenesis Related ◽  
Redlegged Earth Mite ◽  
Ethephon Treatment

One of the chief predators of subterranean clover (Trifolium subterraneum) pastures is redlegged earth mite (Halotydeus destructor; RLEM). Subterranean clover pathogenesis-related (PR) proteins induced by RLEM attack and ethephon treatment were surveyed, and PR proteins with peroxidase, β-1,3-glucanase and chitinase activities were detected. A protein co-migrating with a chitinase activity, induced by RLEM predation and treatment with ethephon, was isolated. It was purified and the N-terminal amino acid sequence determined. Using a degenerate oligonucleotide primer designed from this sequence, a corresponding cDNA fragment was amplified by reverse transcriptase-PCR, then cloned, and used as a probe to screen a subterranean clover cv. Karridale genomic library. The cDNA and a 97% homologous genomic clone were sequenced and the deduced amino acid sequence revealed an open reading frame of 157 amino acids capable of encoding a peptide of 16 478 Da. Significant homology (80%) was found between this protein and an abscisic acid (ABA)-responsive protein from Pisum sativum of unknown function which is an intracellular pathogenesis-related (IPR) protein. The gene encoding this protein also has homology to pea ‘disease response resistance genes’ and to proteins from other plant species in the PR-10 family. The induced protein was designated TsPR-10a due to its homology to other PR-10 proteins. Genomic Southern analysis indicates that the gene encoding this protein, designated Ypr10a, is a member of a multigene family with at least three members. Northern blot analysis indicates that the subterranean clover Ypr10a mRNA, or homologous transcript, level is strongly induced by ethephon treatment in both root and aerial tissues of 3 week old plants. The rapid induction kinetics of Ypr10a mRNA under ethephon treatment, its correlation with a putative chitinase activity, and homology to other PR-protein genes, suggests a pathogenesis-related role for TsPR-10a protein in subterranean clover.

scholarly journals VanD-type glycopeptide-resistant Enterococcus faecium BM4339.

1997 ◽  
Vol 41 (9) ◽  
pp. 2016-2018 ◽  
Author(s):  
B Perichon ◽  
P Reynolds ◽  
P Courvalin
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Enterococcus Faecium ◽  
Glycopeptide Resistance ◽  
Resistance Phenotype ◽  
Resistance Proteins ◽  
Genes Encoding ◽  
New Type ◽  
Low Levels

Enterococcus faecium BM4339 was constitutively resistant to vancomycin (MIC, 64 microg/ml) and to low levels of teicoplanin (MIC, 4 microg/ml). A 605-bp product obtained with the V1 and V2 primers for amplification of genes encoding D-Ala:D-Ala ligases and related glycopeptide resistance proteins was sequenced after cloning. The deduced amino acid sequence had 69% identity with VanA and VanB and 43% identity with VanC, consistent with the finding that BM4339 synthesized peptidoglycan precursors terminating in D-lactate. This new type of glycopeptide resistance phenotype was designated VanD.

scholarly journals Complete Amino Acid Sequence of a Polypeptide from Zea mays Similar to the Pathogenesis-Related-1 Family

1991 ◽  
Vol 96 (4) ◽  
pp. 1372-1375 ◽  
Author(s):  
Jeffrey W. Gillikin ◽  
William Burkhart ◽  
John S. Graham
Keyword(s):  
Zea Mays ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
Pathogenesis Related

The complete amino acid sequence of the pathogenesis-related (PR2) protein induced in chemically stressed bean leaves

1991 ◽  
Vol 1077 (2) ◽  
pp. 241-244 ◽  
Author(s):  
Abalo Awade ◽  
Marie Hélène Metz-Boutigue ◽  
Monique Le Ret ◽  
Geneviève Genot ◽  
Iradj Amiri ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
Pathogenesis Related ◽  
Bean Leaves

VanE, a New Type of Acquired Glycopeptide Resistance in Enterococcus faecalis BM4405

1999 ◽  
Vol 43 (9) ◽  
pp. 2161-2164 ◽  
Author(s):  
Marguerite Fines ◽  
Bruno Perichon ◽  
Peter Reynolds ◽  
Daniel F. Sahm ◽  
Patrice Courvalin
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Degenerate Primers ◽  
Glycopeptide Resistance ◽  
Pcr Product ◽  
New Type ◽  
Total Dna ◽  
Low Levels

ABSTRACT Enterococcus faecalis BM4405 was resistant to low levels of vancomycin (MIC, 16 μg/ml) and was susceptible to teicoplanin (MIC, 0.5 μg/ml). No PCR product was obtained when the total DNA of this clinical isolate was used as a template with primers specific for glycopeptide resistance genes vanA,vanB, vanC, and vanD. However, a 604-bp PCR fragment was obtained when V1 and V2 degenerate primers were used and total DNA was digested with HindIII as a template. The product was cloned and sequenced. The deduced amino acid sequence had greater identity (55%) with VanC than with VanA (45%), VanB (43%), or VanD (44%). This was consistent with the fact that BM4405 synthesized peptidoglycan precursors that terminated ind-serine residues. After induction with vancomycin, weakd,d-dipeptidase and penicillin-insensitived,d-carboxypeptidase activities were detected in cytoplasmic extracts of BM4405, whereas a serine racemase activity was found in the membrane preparation. This new type of acquired glycopeptide resistance was named VanE.

scholarly journals COMPLETE AMINO ACID SEQUENCE OF A NEW TYPE OF NEUROTOXIN FROM THE VENOM OF THE SPIDER, AGELENA OPULENTA

1990 ◽  
Vol 11 (3) ◽  
pp. 181-186 ◽  
Author(s):  
KENICHI HAGIWARA ◽  
TAKESHI SAKAI ◽  
AKIKO MIWA ◽  
NOBUFUMI KAWAI ◽  
TERUMI NAKAJIMA
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Complete Amino Acid Sequence ◽  
New Type
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