scholarly journals Both developmental and metabolic signals activate the promoter of a class I patatin gene

1989 ◽  
Vol 8 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Mario Rocha-Sosa ◽  
Uwe Sonnewald ◽  
Wolf Frommer ◽  
Marina Stratmann ◽  
Jeff Schell ◽  
...  
Keyword(s):  
Class I ◽  
Class I Patatin ◽  
Patatin Gene

The expression of class I patatin gene fusions in transgenic potato varies with both gene and cultivar

1991 ◽  
Vol 16 (1) ◽  
pp. 153-160 ◽  
Author(s):  
K. S. Blundy ◽  
M. A. C. Blundy ◽  
D. Carter ◽  
F. Wilson ◽  
W. D. Park ◽  
...  
Keyword(s):  
Transgenic Potato ◽  
Class I ◽  
Gene Fusions ◽  
Class I Patatin ◽  
Patatin Gene

scholarly journals Organ-specific gene expression in transgenic potato: the cloning a new promoter of a class I patatin gene

1995 ◽  
Vol 11 (6) ◽  
pp. 96-103 ◽  
Author(s):  
I. M. Yefimenko ◽  
T. V. Medvedeva ◽  
P. G. Kovalenko ◽  
K. G. Gazaryan ◽  
A. P. Galkin
Keyword(s):  
Gene Expression ◽  
Transgenic Potato ◽  
Class I ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Organ Specific ◽  
Class I Patatin ◽  
Patatin Gene

Functional analysis of a class I patatin gene SK24-1 in microtuber formation of transgenic potatoes

2008 ◽  
Vol 88 (4) ◽  
pp. 593-598 ◽  
Author(s):  
Huaijun Si ◽  
Jun Liu ◽  
Jian Huang ◽  
Conghua Xie
Keyword(s):  
Escherichia Coli ◽  
Cdna Clone ◽  
Antisense Rna ◽  
Cauliflower Mosaic Virus ◽  
Class I ◽  
35S Promoter ◽  
Transformed Plants ◽  
Transgenic Potatoes ◽  
Class I Patatin

Expression of a class I patatin cDNA clone, SK24-1, in Escherichia coli revealed that the cDNA clone possessed lipid acyl hydrolase (LAH) activity. Transformed potato plants were obtained via Agrobacterium-mediated transformation using the chimeric constructs containing the sense and antisense cDNA under the control cauliflower mosaic virus 35S (CaMV 35S) promoter. In some sense transformed plants, both sense patatin RNA and LAH activity were increased and further resulted in a significant increase of percentage of plantlets that formed microtubers and numbers of microtubers per plantlet in vitro. All antisense plants displayed a reduction in LAH activity. Both sense and antisense RNA could be detected in antisense plants, but transcripts of antisense RNA resulted in a reduction of endogenous sense RNA. Moreover, expression of antisense cDNA in some antisense transformed plants led to a significant decrease in the number of microtubers formed. These results suggest that SK24-1 was involved in regulating microtuber formation. Key words: Patatin, potato, Escherichia coli, sense RNA, antisense RNA

Transformation of potato using an antisense class I patatin gene and its effect on microtuber formation

2005 ◽  
Vol 2 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Si Huai-Jun ◽  
Liu Jun ◽  
Xie Cong-Hua
Keyword(s):  
Pcr Amplification ◽  
Transgenic Potato ◽  
Class I ◽  
Total Soluble Protein ◽  
Northern Hybridization ◽  
35S Promoter ◽  
Antisense Gene ◽  
Potato Genome ◽  
Transformed Plants ◽  
Class I Patatin

AbstractAn antisense class I patatin gene under control of the CaMV 35S promoter was introduced into potato (Solanum tuberosum) cultivar E-potato 3 using the Agrobacterium tumefaciens system. PCR amplification and PCR–Southern blot analysis indicated that the antisense class I patatin gene had been integrated into the potato genome. Northern hybridization analysis showed that the antisense gene transcribed normally in the transgenic potato plants and resulted in a reduction of endogenous class I patatin mRNA. Total soluble protein content and lipid acyl hydrolase activity of microtubers, derived from transformed plants, decreased by a maximum of 36.4% and 31.4%, respectively, compared with control plants. The expression of this antisense gene also resulted in reductions of the plantlets forming tubers, tubers per plantlet and the effective tubers (≥50 mg) of the transformed plants.

Electron microscopy analysis of degenerating pancreatic ß cells in transgenic mice expressing the MHC Class I antigen Dd

1990 ◽  
Vol 48 (3) ◽  
pp. 548-549
Author(s):  
T. A. Stewart ◽  
D. Liggitt ◽  
S. Pitts ◽  
L. Martin ◽  
M. Siegel ◽  
...  
Keyword(s):  
Type I Diabetes ◽  
Disease Process ◽  
Class Ii ◽  
Class I ◽  
Type I ◽  
Aberrant Expression ◽  
Mhc Molecules ◽  
Endogenous Insulin ◽  
Class Ii Mhc ◽  
Ifn Γ

Insulin-dependant (Type I) diabetes mellitus (IDDM) is a metabolic disorder resulting from the lack of endogenous insulin secretion. The disease is thought to result from the autoimmune mediated destruction of the insulin producing ß cells within the islets of Langerhans. The disease process is probably triggered by environmental agents, e.g. virus or chemical toxins on a background of genetic susceptibility associated with particular alleles within the major histocompatiblity complex (MHC). The relation between IDDM and the MHC locus has been reinforced by the demonstration of both class I and class II MHC proteins on the surface of ß cells from newly diagnosed patients as well as mounting evidence that IDDM has an autoimmune pathogenesis. In 1984, a series of observations were used to advance a hypothesis, in which it was suggested that aberrant expression of class II MHC molecules, perhaps induced by gamma-interferon (IFN γ) could present self antigens and initiate an autoimmune disease. We have tested some aspects of this model and demonstrated that expression of IFN γ by pancreatic ß cells can initiate an inflammatory destruction of both the islets and pancreas and does lead to IDDM.

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