Type I collagen regulated dentin matrix protein-1 (Dmp-1) and osteocalcin (OCN) gene expression of rat dental pulp cells

2003 ◽  
Vol 88 (6) ◽  
pp. 1112-1119 ◽  
Author(s):  
Morimichi Mizuno ◽  
Tetsuro Miyamoto ◽  
Keinoshin Wada ◽  
Sanae Watatani ◽  
Gui Xia Zhang
Keyword(s):  
Gene Expression ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Type I Collagen ◽  
Type I ◽  
Dental Pulp Cells ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Pulp Cells ◽  
Dentin Matrix

scholarly journals Dentin matrix protein 1 (DMP1) expression in developing human teeth

2009 ◽  
Vol 20 (5) ◽  
pp. 365-369 ◽  
Author(s):  
Elizabeth Ferreira Martinez ◽  
Luciana Alves Herdy da Silva ◽  
Cristiane Furuse ◽  
Ney Soares de Araújo ◽  
Vera Cavalcanti de Araújo
Keyword(s):  
Dental Pulp ◽  
Matrix Protein ◽  
Enamel Organ ◽  
Dentin Matrix Protein 1 ◽  
Tooth Formation ◽  
Dentinal Tubules ◽  
Dental Lamina ◽  
Dentin Matrix Protein ◽  
Dental Papilla ◽  
Dentin Matrix

Dentin matrix protein 1 (DMP1) is an acidic phosphoprotein that plays an important role in mineralized tissue formation by initiation of nucleation and modulation of mineral phase morphology. The purpose of the present study was to examine the immunoexpression of DMP1 in tooth germs of 7 human fetuses at different gestational ages (14, 16, 19, 20, 21, 23 and 24 weeks) comparing with completed tooth formation erupted teeth. The results showed the presence of DMP1 in the dental lamina, as well as in the cells of the external epithelium, stellate reticulum and stratum intermedium of the enamel organ. However, in the internal dental epithelium, cervical loop region and dental papilla some cells have not labeled for DMP1. In the crown stage, DMP1 was expressed in the ameloblast and odontoblast layer, as well as in the dentinal tubules of coronal dentin near the odontoblast area. Erupted teeth with complete tooth formation exhibited immunolabeling for DMP1 only in the dentinal tubules mainly close to the dental pulp. No staining was observed in the enamel, predentin or dental pulp matrix. DMP1 is present in all developing dental structures (dental lamina, enamel organ, dental papilla) presenting few immunoexpression variations, with no staining in mineralized enamel and dentin.

scholarly journals Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Shihui Jiang ◽  
Zhaoxia Yu ◽  
Lanrui Zhang ◽  
Guanhua Wang ◽  
Xiaohua Dai ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Dental Pulp ◽  
Type I Collagen ◽  
Type I ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Alp Activity ◽  
Proliferation And Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Abstract This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.

Dentin Matrix Protein-1 Activates Dental Pulp Fibroblasts

2012 ◽  
Vol 38 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Ashraf Abd-Elmeguid ◽  
Donald C. Yu ◽  
Loren W. Kline ◽  
Redwan Moqbel ◽  
Harissios Vliagoftis
Keyword(s):  
Dental Pulp ◽  
Matrix Protein ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Dentin Matrix

scholarly journals Biomimetic Approach to Perforation Repair Using Dental Pulp Stem Cells and Dentin Matrix Protein 1

2011 ◽  
Vol 37 (8) ◽  
pp. 1092-1097 ◽  
Author(s):  
Rajaa Alsanea ◽  
Sriram Ravindran ◽  
Mohamed I. Fayad ◽  
Bradford R. Johnson ◽  
Christopher S. Wenckus ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Dental Pulp Stem Cells ◽  
Dentin Matrix Protein 1 ◽  
Dentin Matrix Protein ◽  
Dentin Matrix ◽  
Biomimetic Approach

scholarly journals Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta

2014 ◽  
Author(s):  
G. Orsini ◽  
A. Majorana ◽  
A. Mazzoni ◽  
A. Putignano ◽  
M. Falconi ◽  
...  
Keyword(s):  
Osteogenesis Imperfecta ◽  
Matrix Protein ◽  
Primary Teeth ◽  
Type I Collagen ◽  
Immunohistochemical Analysis ◽  
Matrix Proteins ◽  
Type I ◽  
Dentinogenesis Imperfecta ◽  
Dentin Matrix Protein ◽  
Dentin Matrix

Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P<0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins.

Hyper-expression of Osteocalcin mRNA in Odontoblasts of Hyp Mice

2005 ◽  
Vol 84 (1) ◽  
pp. 84-88 ◽  
Author(s):  
T. Onishi ◽  
T. Ogawa ◽  
T. Hayashibara ◽  
T. Hoshino ◽  
R. Okawa ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Type I Collagen ◽  
Tooth Germ ◽  
Type I ◽  
Wild Type ◽  
Gene Expressions ◽  
Dentin Matrix Protein ◽  
Osteocalcin Gene ◽  
Dentin Matrix

The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontn, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization.

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