Photoaffinity labeling of the N-formyl peptide receptor of human polymorphonuclear leukocytes

1982 ◽  
Vol 20 (2) ◽  
pp. 203-214 ◽  
Author(s):  
Richard G. Painter ◽  
Manfred Schmitt ◽  
Algirdas J. Jesaitis ◽  
Larry A. Sklar ◽  
Klaus Preissner ◽  
...  
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Photoaffinity Labeling ◽  
Formyl Peptide Receptor ◽  
Peptide Receptor ◽  
Formyl Peptide ◽  
Human Polymorphonuclear Leukocytes

Design and characterization of a cleavage-resistant Annexin A1 mutant to control inflammation in the microvasculature

Blood ◽  
2010 ◽  
Vol 116 (20) ◽  
pp. 4288-4296 ◽  
Author(s):  
Magali Pederzoli-Ribeil ◽  
Francesco Maione ◽  
Dianne Cooper ◽  
Adam Al-Kashi ◽  
Jesmond Dalli ◽  
...  
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Early Stage ◽  
Leukocyte Adhesion ◽  
Formyl Peptide Receptor ◽  
Annexin A1 ◽  
Formyl Peptide ◽  
Anti Inflammatory ◽  
Human Polymorphonuclear Leukocytes

Abstract Human polymorphonuclear leukocytes adhesion to endothelial cells during the early stage of inflammation leads to cell surface externalization of Annexin A1 (AnxA1), an effector of endogenous anti-inflammation. The antiadhesive properties of AnxA1 become operative to finely tune polymorphonuclear leukocytes transmigration to the site of inflammation. Membrane bound proteinase 3 (PR3) plays a key role in this microenvironment by cleaving the N terminus bioactive domain of AnxA1. In the present study, we generated a PR3-resistant human recombinant AnxA1—named superAnxA1 (SAnxA1)—and tested its in vitro and in vivo properties in comparison to the parental protein. SAnxA1 bound and activated formyl peptide receptor 2 in a similar way as the parental protein, while showing a resistance to cleavage by recombinant PR3. SAnxA1 retained anti-inflammatory activities in the murine inflamed microcirculation (leukocyte adhesion being the readout) and in skin trafficking model. When longer-lasting models of inflammation were applied, SAnxA1 displayed stronger anti-inflammatory effect over time compared with the parental protein. Together these results indicate that AnxA1 cleavage is an important process during neutrophilic inflammation and that controlling the balance between AnxA1/PR3 activities might represent a promising avenue for the discovery of novel therapeutic approaches.

scholarly journals Recent advances in the design and development of formyl peptide receptor 2 (FPR2/ALX) agonists as pro-resolving agents with diverse therapeutic potential

2021 ◽  
Vol 213 ◽  
pp. 113167
Author(s):  
Monika Maciuszek ◽  
Antonino Cacace ◽  
Eoin Brennan ◽  
Catherine Godson ◽  
Timothy M. Chapman
Keyword(s):  
Therapeutic Potential ◽  
Formyl Peptide Receptor ◽  
Design And Development ◽  
Peptide Receptor ◽  
Recent Advances ◽  
Formyl Peptide ◽  
Resolving Agents
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