Applying eco‐friendly micellar liquid chromatography for the simultaneous determination of two ternary mixtures utilized for cold treatment using monolithic column

2021 ◽  
Author(s):  
Mohamed A. Abu El‐Enin ◽  
Yomna A. Salem ◽  
Saadia M. El‐Ashry ◽  
Mohammed E. A. Hammouda
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Monolithic Column ◽  
Cold Treatment ◽  
Micellar Liquid Chromatography ◽  
Ternary Mixtures

scholarly journals Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Mahmoud Mohamed Issa ◽  
R'afat Mahmoud Nejem ◽  
Alaa Abu Shanab ◽  
Raluca-Ioana Stefan-van Staden
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ternary Mixture ◽  
Standard Addition ◽  
Ternary Mixtures ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
The Matrix ◽  
Crossing Point

Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5–20 μg mL−1 of aspirin, 2.5–17.5 μg mL−1 of atorvastatin, and 2.5–20 μg mL−1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212 nm, while for atorvastatin the zero crossing point at 250 nm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5–20 μg mL−1 aspirin, 2.5–17.5 μg mL−1 atorvastatin and 2.5–20 μg mL−1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography.

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