Prenatal sexing and detection of zfy gene sequences in sex chromosome disorders by polymerase chain reaction

In this report we analyse the effects of distamycin and five distamycin analogues on amplification by polymerase-chain reaction (PCR) of two gene sequences displaying a different A+T/G+C content. The first was a 5′ region of the human oestrogen receptor (ER) gene, containing a (TA)26 stretch; the second was a CG-rich sequence of the human Ha-ras oncogene. The results obtained unequivocally demonstrate that the addition of one pyrrole ring significantly improves the ability of distamycin derivatives to interfere with PCR-mediated amplification of the human ER genomic region carrying a (TA)26 stretch. The distamycin analogues analysed differ in the number of pyrrole rings and in the presence of an N-formyl, an N-formimidoyl or a retroamide group at position X1. Among compounds carrying the same number of pyrrole rings, those carrying an N-formyl or an N-formimidoyl group retain a similar inhibitory activity. The retroamide analogues, on the contrary, are much less efficient in inhibiting PCR-mediated amplification of the 5′ER region. With respect to sequence selectivity both distamycin and distamycin analogues exhibit a sequence preference, since they do not inhibit PCR amplification of Ha-ras CG-rich gene regions, with the exception of a distamycin analogue carrying four pyrrole rings.

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Sex determination in ostrich (Struthio camelus) using DNA markers

Canadian Journal of Animal Science ◽

10.4141/cjas09125 ◽

2010 ◽

Vol 90 (3) ◽

pp. 357-360 ◽

Cited By ~ 1

Author(s):

M. Alipanah ◽

A. Torkamanzehi ◽

H. Taghavi

Keyword(s):

Polymerase Chain Reaction ◽

Sexual Dimorphism ◽

Sex Determination ◽

Sex Chromosome ◽

Molecular Genetic ◽

Bird Species ◽

Rapid Identification ◽

Chain Reaction ◽

Struthio Camelus ◽

Polymerase Chain

Production of bird species such as ostrich (Struthio camelus) has been gaining increasing importance in Iran as well as many other countries. Ostrich, similar to many other species of birds, lacks sexual dimorphism, making it difficult to differentiate between males and females, especially at an early age, which can be problematic in breeding programs. Recently developed molecular genetic methods that utilize polymerase chain reaction (PCR) based techniques can facilitate rapid identification of the bird’s sex in these species using a DNA sample, which can be easily extracted from blood or feather pulps. We successfully applied a PCR-based RFLP technique and sex chromosome primers for sex determination in a sample of 30 Ostrich chicks using DNA extracted from blood and feather pulps. Both DNA samples (blood and feather pulps) provided useful results. However, using feather pulps from 1-day-old chicks can provide an easy and inexpensive method for sex determination in ostrich. Key words: Ostrich (struthio camelus), sex determination, sexual dimorphism, polymerase chain reaction, RFLP

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Polymerase chain reaction–hybridization method using urease gene sequences for high-throughput Ureaplasma urealyticum and Ureaplasma parvum detection and differentiation

Analytical Biochemistry ◽

10.1016/j.ab.2016.01.018 ◽

2016 ◽

Vol 499 ◽

pp. 57-62 ◽

Cited By ~ 3

Author(s):

Chen Xu ◽

Nan Zhang ◽

Qianyu Huo ◽

Minghui Chen ◽

Rengfeng Wang ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

High Throughput ◽

Ureaplasma Urealyticum ◽

Hybridization Method ◽

Gene Sequences ◽

Chain Reaction ◽

Ureaplasma Parvum ◽

Urease Gene ◽

Polymerase Chain

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Molecular characterization and detection of variants of Taenia multiceps in sheep in Turkey

Parasitology ◽

10.1017/s0031182016001669 ◽

2016 ◽

Vol 144 (2) ◽

pp. 220-225 ◽

Cited By ~ 2

Author(s):

BETUL SONMEZ ◽

ERGUN KOROGLU ◽

SAMI SIMSEK

Keyword(s):

Central Nervous System ◽

Polymerase Chain Reaction ◽

Adult Stage ◽

Infected Sheep ◽

Gene Sequences ◽

Chain Reaction ◽

Taenia Multiceps ◽

Coenurus Cerebralis ◽

The Central Nervous System ◽

Polymerase Chain

SUMMARYTaenia multiceps is a cestode (family Taeniidae) that in its adult stage lives in the small intestine of dogs and other canids. The metacestode, known as Coenurus cerebralis, is usually found in the central nervous system including brain and spinal card in sheep and other ruminants. The presence of cysts typically leads to neurological symptoms that in the majority of cases result in the death of the animal. Coenurosis could cause high losses in sheep farms because the disease commonly affects young animals. A total of 20 C. cerebralis isolates collected from naturally infected sheep in Mardin province of Turkey were characterized through the polymerase chain reaction and sequencing of a fragment of cytochrome c oxidase subunit 1 (CO1) gene. The results showed that the CO1 gene sequences were highly conserved in C. cerebralis isolates. Phylogenetic analysis based on partial CO1 gene sequences revealed that C. cerebralis isolates were composed of three different variants.

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The Polymerase Chain Reaction (PCR) to Detect Gene Sequences of Borrelia Burgdorferi, the Etiologic Agent of Lyme Disease

PCR Topics ◽

10.1007/978-3-642-75924-6_37 ◽

1991 ◽

pp. 206-208

Author(s):

Sabine E. Moter ◽

Michael D. Kramer ◽

Markus M. Simon ◽

Ullrich E. Schaible ◽

R. Kinzelbach ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Lyme Disease ◽

Borrelia Burgdorferi ◽

Etiologic Agent ◽

Gene Sequences ◽

Chain Reaction ◽

Polymerase Chain

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Determination of gender in cetaceans by the polymerase chain reaction

Canadian Journal of Zoology ◽

10.1139/z92-292 ◽

1992 ◽

Vol 70 (11) ◽

pp. 2166-2170 ◽

Cited By ~ 97

Author(s):

Per J. Palsbøll ◽

Anna Vader ◽

Ingrid Bakke ◽

M. Raafat El-Gewely

Keyword(s):

Polymerase Chain Reaction ◽

Sex Chromosome ◽

Chain Reaction ◽

Cetacean Species ◽

Simple Method ◽

Fin Whale ◽

Skin Biopsies ◽

Polymerase Chain ◽

Free Ranging

We determined the gender of a variety of cetacean species, including both ondotocetes and mysticetes, using the polymerase chain reaction for amplification of the sex chromosome specific regions ZFY/ZFX and SRY. This quick and simple method requires extremely small amounts of tissue, and therefore allows gender to be determined from skin biopsies taken from free-ranging specimens. In the fin whale, Balaenoptera physalus, no gender-specific bands were observed when the ZFY/ZFX system was used, but when the SRY system was used, sex was accurately determined. Previous studies in other mammals have also shown the SRY system to be more reliable in sex determination. We therefore recommend amplification of the SRY region alone or in parallel with the ZFY/ZFX regions, as described here, as a test for gender in cetaceans and other mammals.

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