Commitment and proliferation kinetics of human lymphocytes stimulated in vitro: Effects of colchicine on mitogen response

1982 ◽  
Vol 112 (2) ◽  
pp. 157-161 ◽  
Author(s):  
David J. Hall ◽  
James J. O'leary ◽  
Andreas Rosenberg
Keyword(s):  
Human Lymphocytes ◽  
Proliferation Kinetics ◽  
Mitogen Response ◽  
In Vitro Effects ◽  
Kinetics Of

In Vitro Effects of Albendazole and Its Metabolites on the Cell Proliferation Kinetics and Micronuclei Frequency of Stimulated Human Lymphocytes

2001 ◽  
Vol 32 (2) ◽  
pp. 119-122 ◽  
Author(s):  
Tzutzuy Ramı́rez ◽  
Luis Benı́tez-Bribiesca ◽  
Patricia Ostrosky-Wegman ◽  
Luis A Herrera
Keyword(s):  
Cell Proliferation ◽  
Human Lymphocytes ◽  
Proliferation Kinetics ◽  
In Vitro Effects

scholarly journals Protein Synthesis in Human Leukocytes and Lymphocytes: 1. Effect of Steroids and Sterols

Blood ◽  
1969 ◽  
Vol 34 (3) ◽  
pp. 348-356 ◽  
Author(s):  
SEYMOUR WERTHAMER ◽  
CARL HICKS ◽  
LEONARD AMARAL
Keyword(s):  
Protein Synthesis ◽  
Steroid Hormones ◽  
Plasma Protein ◽  
Human Lymphocytes ◽  
Amino Acid Incorporation ◽  
Binding Factor ◽  
In Vitro Effects ◽  
Physiologic Role

Abstract The in vitro effects of sterols, cholesterol and 3-methyl cholanthrene and steroids, cortisol, prednisolone and testosterone on protein synthesis in separate popultions of human lymphocytes and leukocytes has been investigated. It has been shown that all agents used result in the inhibition of protein synthesis under these conditions. It has also been shown that the inhibitory mechanism of the steroid hormones requires the presence of plasma, presumably as a protein binding factor in order to achieve its effect. The sterol, cholesterol and 3-methyl cholanthrene, in the absence of plasma, still inhibit amino acid incorporation. However, in the case of cholesterol, the magnitude of inhibition is lower than that observed in the presence of plasma, perhaps indicating a partial plasma dependence. The results presented therefore support the hypothesis that the inhibition of lymphocyte protein synthesis by steroid hormones occurs only when the steroid is bound to a plasma protein. The physiologic role of the plasma protein-cortisol complex and its relation to the condition of lymphopenia in man is discussed.

In Vivo and in Vitro Proliferation Kinetics of Sarcoma 180 in Solid Form

1972 ◽  
Vol 58 (5) ◽  
pp. 335-339 ◽  
Author(s):  
Rosella Silvestrini ◽  
Ornella Sanfilippo ◽  
Luigi Lenaz
Keyword(s):  
Double Labelling ◽  
Pulse Labelling ◽  
Sarcoma 180 ◽  
In Vitro Proliferation ◽  
Proliferation Kinetics ◽  
Human Solid Tumors ◽  
A Cell ◽  
Kinetics Of

In order to obtain data for setting up a rapid and relatively inexpensive method for studying the proliferation kinetics of human solid tumors, we have determined the kinetic parameters of an experimental solid tumor (Sarcoma 180). The curve of labelled mitosis after pulse labelling with 3H thymidine and the 3H and 14C thymidine double labelling technic on tumor samples incubated in vitro with the labelled precursors were used. A method of digestion of the tissue with hyaluronidase to obtain a cell suspension is described. This method allows easy identification of cells labelled with 3H or 14C thymidine. The two methods yielded reproducible results, the labelling index being 45%, and the duration of S phase 9.9 hours. The in vitro double labelling method with subsequent hyaluronidase digestion is proposed for studying the proliferation kinetics of solid malignancies.

scholarly journals Detection of Paralytic Shellfish Poison by Rapid Cell Bioassay: Antagonism of Voltage-Gated Sodium Channel Active Toxins in vitro

2003 ◽  
Vol 86 (3) ◽  
pp. 540-543 ◽  
Author(s):  
Ronald L Manger ◽  
Linda S Leja ◽  
Sue Y Lee ◽  
James M Hungerford ◽  
Mary Ann Kirkpatrick ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Mouse Bioassay ◽  
Marine Toxins ◽  
Prolonged Incubation ◽  
Paralytic Shellfish Poison ◽  
Paralytic Shellfish ◽  
Channel Blocking ◽  
In Vitro Effects ◽  
Kinetics Of

Abstract Although cytotoxicity assays provide several advantages over mouse bioassays, sodium channel-blocking marine toxins, such as those associated with paralytic shellfish poison (PSP), require prolonged incubation periods of 24–48 h. This is in marked contrast to in vitro detection of sodium channel-enhancing marine toxins such as ciguatoxins or brevetoxins which can be accomplished in as few as 4–6 h. We developed a modified PSP cell bioassay that is as rapid as in vitro methods for sodium channel-enhancing toxins. The cell bioassay is based on a saxitoxin-dependent antagonism of the rapid in vitro effects of brevetoxin or ciguatoxin. Comparative analysis of naturally incurred PSP residues by both antagonism cell bioassay and the mouse bioassay demonstrated significant correlation. The simplicity, sensitivity, and enhanced kinetics of the new antagonism cell bioassay format provide the basis for development of a practical alternative to conventional mouse testing for PSP.

In vitro study on proliferation kinetics of oral mucosal keratinocytes

2015 ◽  
Vol 120 (4) ◽  
pp. 429-435 ◽  
Author(s):  
Janike Dickhuth ◽  
Steffen Koerdt ◽  
Ulrike Kriegebaum ◽  
Christian Linz ◽  
Urs D. Müller-Richter ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Proliferation Kinetics ◽  
Kinetics Of

In vitro effects of mycophenolic acid on cell cycle and activation of human lymphocytes

2000 ◽  
Vol 300 (1-2) ◽  
pp. 23-28 ◽  
Author(s):  
Angelika Heinschink ◽  
Markus Raab ◽  
Heide Daxecker ◽  
Andrea Griesmacher ◽  
Mathias M Müller
Keyword(s):  
Cell Cycle ◽  
Mycophenolic Acid ◽  
Human Lymphocytes ◽  
In Vitro Effects

scholarly journals Kinetics of nuclear phosphorylation ( -H2AX) in human lymphocytes treated in vitro with UVB, bleomycin and mitomycin C

Mutagenesis ◽  
2013 ◽  
Vol 28 (4) ◽  
pp. 465-473 ◽  
Author(s):  
R. Scarpato ◽  
S. Castagna ◽  
R. Aliotta ◽  
A. Azzara ◽  
F. Ghetti ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Human Lymphocytes ◽  
Kinetics Of

Induktion von Einzel-und Doppelstrangbrüchen in linearer und superzirkularer DNA durch Phleomycin / Induction of Single-and Double-Strand Breaks in Linear and Superhelical DNA by Phleomycin

1982 ◽  
Vol 37 (11-12) ◽  
pp. 1228-1233 ◽  
Author(s):  
Franz Schmülling ◽  
Wolfgang Köhnlein
Keyword(s):  
Human Lymphocytes ◽  
Dna Interaction ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Dna Breakage ◽  
Single Strand Breaks ◽  
Breakage Rate ◽  
Strand Breakage ◽  
In Vitro Effects

Abstract Phleomycin induced DNA breakage was investigated with superhelical Col. E1 DNA and linear T2 DNA as well. In both DNA-forms besides single-strand breaks direct double-strand breaks were produced by the drug. The double-strand breakage rate obtained after treatment with phleomycin, however, was considerably smaller than that found after bleomycin treatment. Whereas the single-and double-strand breakage rate in Col. E1 DNA showed a linear and nearly quadratic dependence on the phleomycin concentration, respectively, in T2 DNA the breakage rates increased faster than the first or second power of the concentration. This indicates various modes of drug-DNA interaction. Under nondegrading conditions a strong retardation of electrophoretic mobility was observed for all three topological isomers of Col. E1 DNA whereas the sedimentation behaviour remained unchanged. The in vitro effects (strand breakage) of phleomycin and bleomycin are compared with in­ duction of chromosomal aberrations in human lymphocytes and oocytes of Drosophila melanogaster.

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