Transmission and scanning electron microscopic examination of intracellular organelles in freeze-substitutedKloeckera andSaccharomyces cerevisiae Yeast Cells

1987 ◽  
Vol 5 (3) ◽  
pp. 249-261 ◽  
Author(s):  
Misuzu Baba ◽  
Masako Osumi
Keyword(s):  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Yeast Cells ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Intracellular Organelles ◽  
Scanning Electron

A comparison of the effects of several antifungal imidazole derivatives and polyenes on Candida albicans: an ultrastructural study by scanning electron microscopy

1982 ◽  
Vol 28 (10) ◽  
pp. 1119-1126 ◽  
Author(s):  
M. Bastide ◽  
S. Jouvert ◽  
J.-M. Bastide
Keyword(s):  
Electron Microscopy ◽  
Cell Wall ◽  
Candida Albicans ◽  
Cytoplasmic Membrane ◽  
Electron Microscopic Examination ◽  
Yeast Cells ◽  
Electron Microscopic ◽  
Imidazole Derivatives ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

The early events in the interaction of two polyene (amphotericin B and nystatin) and five imidazole (clotrimazole, ketoconazole, miconazole, isoconazole, and econazole) antimycotics used at fungicidal concentrations with the surface of Candida albicans were studied by scanning electron microscopic examination of treated intact young yeast cells, treated spheroplasts, and spheroplasts liberated from treated young yeast cells. In all cases, treatment lasted 2 h. The polyenes passed through the yeast cell wall and interacted with the cytoplasmic membrane causing the spheroplasts to lose their characteristic spheric form and to liberate their contents. Clotrimazole caused the formation of numerous circular openings in the cytoplasmic membrane, but only when the agent was used to treat spheroplasts directly. Ketoconazole, miconazole, isoconazole, and econazole interacted with the cell wall causing formation of convolutions and wrinkles. The three imidazole derivatives that are structurally closely related, miconazole, isoconazole, and econazole, inhibited the enzyme-catalyzed release of spheroplasts from young yeast cells.

Developmental Disturbances of the Rat Molar Induced By Two Diphosphonates

1989 ◽  
Vol 3 (2) ◽  
pp. 234-240 ◽  
Author(s):  
N. Fouda ◽  
M. Caracatsanis ◽  
L. Hammarstrom
Keyword(s):  
Microscopic Examination ◽  
Alveolar Bone ◽  
Morphological Changes ◽  
Electron Microscopic Examination ◽  
Enamel Matrix ◽  
Electron Microscopic ◽  
Developmental Disturbances ◽  
Scanning Electron Microscopic ◽  
Rat Molar ◽  
Scanning Electron

Very few reports have been published about the effects of diphosphonates on the cells and tissues of developing teeth. The present study was designed to investigate possible morphological changes in ameloblasts and odontoblasts and relate these changes to defects in the enamel surface of erupted teeth. Young rats were injected subcutaneously with single or multiple doses of HEDP or Cl2MDP (10 mg P/kg b.w.). Light microscopic examination of developing maxillary first molars showed that single injections of HEDP or Cl2MDP induced subameloblastic cysts between the secretory ameloblasts and the developing enamel. The ameloblastic lining of the cysts contained numerous calcified deposits. A few days after injection, hypoplasias were seen in the enamel in areas previously occupied by cysts. In the erupted teeth, scanning electron microscopic examination revealed enamel hypoplasias which were mainly localized on the mesial sides of the cusps. In addition to the previously mentioned disturbances, multiple injections resulted in more extensive cysts, some of which contained non-mineralized enamel matrix. Inhibition of the mineralization of dentin and alveolar bone was also noticed.

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