Use of immunogold-silver staining to visualize antibody-antigen complexes on LR white embedded tissues prior to electron microscopy

1990 ◽  
Vol 16 (1) ◽  
pp. 83-84 ◽  
Author(s):  
John R. Barta ◽  
Jean Corbin
Keyword(s):  
Electron Microscopy ◽  
Silver Staining ◽  
Lr White

Factors influencing adhesive bonding at the bone/implant interface

1992 ◽  
Vol 50 (2) ◽  
pp. 1114-1115
Author(s):  
Julie A. Martini ◽  
Robert H. Doremus
Keyword(s):  
Electron Microscopy ◽  
Adhesive Bonding ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Bone Implant ◽  
Factors Influencing ◽  
Lr White ◽  
Transmission Electron ◽  
New Zealand White Rabbits ◽  
Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

Esophageal Collision Tumor (Large Cell Neuroendocrine Carcinoma and Papillary Carcinoma) Arising in a Barrett Esophagus

2000 ◽  
Vol 124 (3) ◽  
pp. 411-415 ◽  
Author(s):  
Christopher I. Wilson ◽  
Janna Summerall ◽  
Irvin Willis ◽  
Jack Lubin ◽  
Beria Cabello Inchausti
Keyword(s):  
Electron Microscopy ◽  
Neuroendocrine Carcinoma ◽  
Silver Staining ◽  
Large Cell ◽  
Collision Tumor ◽  
Papillary Adenocarcinoma ◽  
Large Cell Neuroendocrine Carcinoma ◽  
Barrett Esophagus ◽  
Unique Case ◽  
Hematoxylin Eosin

Abstract We report herein a unique case of an esophageal collision tumor composed of a papillary adenocarcinoma and a large cell neuroendocrine carcinoma arising in a Barrett esophagus. Hematoxylin-eosin and silver staining patterns, immunohistochemistry, and electron microscopy of the large cell neuroendocrine component are discussed.

Silver staining of NORs in electron microscopy

1979 ◽  
Vol 123 (2) ◽  
pp. 449-452 ◽  
Author(s):  
C.A. Bourgeois ◽  
D. Hernandez-Verdun ◽  
J. Hubert ◽  
M. Bouteille
Keyword(s):  
Electron Microscopy ◽  
Silver Staining

scholarly journals Combined use of silver staining of the retrograde tracer WGAapoHRP-Au and pre-embedding immunocytochemistry for electron microscopy: demonstration of dopaminergic terminals in synaptic contact with striatal neurons projecting to the substantia nigra in the rat.

1992 ◽  
Vol 40 (6) ◽  
pp. 889-892 ◽  
Author(s):  
R Arai ◽  
Y Kojima ◽  
M Geffard ◽  
K Kitahama ◽  
T Maeda
Keyword(s):  
Electron Microscopy ◽  
Substantia Nigra ◽  
Silver Staining ◽  
Synaptic Contact ◽  
Immunoperoxidase Staining ◽  
Immunoperoxidase Technique ◽  
Striatal Neurons ◽  
Combined Use ◽  
Cryostat Sections ◽  
The Brain

We investigated the applicability of the pre-embedding immunoperoxidase technique to WGAapoHRP-Au retrograde tracing. After injection of the tracer into the substantia nigra of rat, the brain was fixed and cryostat sections were immunostained for dopamine. The sections were osmicated and silver-stained to amplify the colloidal gold particles. Products of both the immunoperoxidase staining and the silver staining could be detected and distinguished by electron microscopy at low magnification. The ultrastructure was so well preserved that synaptic characteristics could be investigated. Dopaminergic terminals were demonstrated to synapse with striatal neurons projecting to the substantia nigra.

The immunogold-silver staining approach in the study of lymphocyte subpopulations in transmission electron microscopy

1990 ◽  
Vol 128 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Gian Carlo Manara ◽  
Corrado Ferrari ◽  
Claudio Torresani ◽  
Paolo Sansoni ◽  
Giuseppe De Panfilis
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Silver Staining ◽  
Lymphocyte Subpopulations ◽  
Transmission Electron

Investigation of immunogold-silver staining by electron microscopy

1985 ◽  
Vol 83 (6) ◽  
pp. 545-550 ◽  
Author(s):  
P. M. Lackie ◽  
R. J. Hennessy ◽  
G. W. Hacker ◽  
J. M. Polak
Keyword(s):  
Electron Microscopy ◽  
Silver Staining
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