Morpho‐palynological assessment of some species of family Asteraceae and Lamiaceae of District Bannu, Pakistan on the bases of light microscope & scanning electron microscopy

Author(s):  
Siraj Khan ◽  
Gul Jan ◽  
Mushtaq Ahmad ◽  
Farzana Gul ◽  
Muhammad Zafar ◽  
...  
2020 ◽  
Vol 11 (2) ◽  
pp. 486-492
Author(s):  
Jens Anibal Juul ◽  
Vegard Asgeir Forsaa ◽  
Tor Paaske Utheim ◽  
Endre Willassen

We present a case report of periocular Loa loa. The key feature of L. loa distinguishing it from other human filarial parasites are cuticular bosses, which are presented in images from a light microscope and a scanning electron microscope. The cuticular bosses could be divided into three subtypes not previously described.


1985 ◽  
Vol 63 (12) ◽  
pp. 2357-2363 ◽  
Author(s):  
M. J. C. Rhodes ◽  
R. J. Robins ◽  
R. J. Turner ◽  
J. I. Smith

The surface features of plant cells immobilised in a matrix of either reticulated polyurethane foam or nylon fibre have been examined with the scanning electron microscope. It has been found that both cells and matrix are enveloped in a thin film, the appearance of which is very dependent on the method by which material is prepared for scanning electron microscopy. The structure is severely damaged by fixation and dehydration. Only in specimens examined in the frozen hydrated state is a structure seen compatible with that observed with the light microscope. From the way the appearance of the film is affected by different methods of preparation for the scanning electron microscope, it is suggested that the film is a hydrated mucilage. The importance of this film for the retention of cells within the matrix is discussed.


1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species


1983 ◽  
Vol 61 (7) ◽  
pp. 2047-2051 ◽  
Author(s):  
J. A. Traquair ◽  
E. G. Kokko

Scanning electron microscopy of the urediniospores of three Puccinia species further resolved their spore ornamentation and supported observations made with the light microscope. In particular, the smooth hilum surface of P. jaceae Otth. urediniospores was clearly observed in contrast to the minutely verrucose and distinctly verrucose hilum of P. centaureae D. C. and P. carthami Cda., respectively. These observations in combination with other morphological features of urediniospores were useful in delimiting the three species and in recognizing two urediniospore forms characteristic of separate evolutionary lineages.


1968 ◽  
Vol 100 (1) ◽  
pp. 1-4 ◽  
Author(s):  
E. H. Salkeld ◽  
A. Wilkes

A recent development in microscopy certain to be of great interest to entomologists is the Scanning Electron Microscope. This machine overcomes the difficulties of studying solid surfaces with a standard light microscope and the problems of the extremely small limits of penetration of the electron microscope. This new microscope focuses a stream of electrons into a beam as small as 1 μ in diameter which moves over the surface of the specimen in a regular pattern, causing secondary radiations to emerge from the surface of the specimen. These are collected by a very sensitive detector and converted to an image similar to that produced by a television tube.


1985 ◽  
Vol 77 (1) ◽  
pp. 143-153
Author(s):  
C.J. Harrison ◽  
E.M. Jack ◽  
T.D. Allen ◽  
R. Harris

A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding was confirmed by a positional correlation between the G-positive bands observed in the light microscope and the circumferential grooves between the quaternary coils of the metaphase chromosomes, observed in the SEM. In further support of this the regions between the grooves showed a positional relationship with the G-negative or reverse (R) bands. The examination of slightly extended metaphase chromosomes in the light microscope demonstrated that the G-banding pattern corresponded to that described by the Paris nomenclature for metaphase chromosomes. The arrangement of the circumferential grooves of the same chromosomes, observed in the SEM, was shown to relate to that described by the Paris nomenclature for prometaphase chromosomes. Therefore, using the SEM it is possible to demonstrate the details of prometaphase banding in metaphase chromosomes.


Author(s):  
S. K. Dutta ◽  
R. B. Johnson

Studies were conducted with chicken tracheas infected in vivo and in vitro with infectious bronchitis virus. For vivo studies, chickens were infected intratracheally with the virus. At intervals, they were sacrificed, tracheal rings were cut in 1-1.5 mm widths, washed, examined in the light microscope for ciliary movement and processed for scanning electron microscopy (SEM). For in vitro studies, tracheal rings of similar dimension were planted in tissue culture media and were infected with the virus. At intervals the tracheal rings were examined in the light microscope for ciliary movement and processed for SEM.


Author(s):  
Judith A. Murphy

Taxonomy of the Myxomyoetes has been based primarily on morphological characteristics observed in the light microscope. Although the fruiting stage is sensitive to environmental conditions, most species exhibit a distinctive although variable category, and thus characteristics of this stage have been used for morphological differentiation. There has been and still is, however, much disagreement concerning the classification and relationships between members of this group. Although capillitial and spore morphology are used in taxonomic classifications, because of their small dimensions they are often poorly defined or not visible in the light microscope. These characteristics are easily discernible by surface scanning electron microscopy (SEM). The purpose of this initial study was to investigate appropriate sample preparations and determine if taxonomically valuable information could be provided with the use of SEM.


Nematology ◽  
2005 ◽  
Vol 7 (2) ◽  
pp. 249-252 ◽  
Author(s):  
Etienne Geraert ◽  
Dieter Sturhan

AbstractLight-microscope studies revealed the presence of phasmids in the mid-tail region in species of the genera Tylenchulus, Trophotylenchulus (syn. Trophonema), Sphaeronema and Meloidoderita in Tylenchulidae. Scanning electron microscopy studies on Sphaeronema species verified these observations. Absence of phasmids or phasmid-like structures was confirmed for genera placed in Paratylenchinae and Tylenchocriconematinae in the same family, and for other taxa of Criconematoidea. This first record of phasmids in the superfamily Criconematoidea requires reconsideration of the systematic position of Tylenchulidae and of the relationships of the genera currently placed in this family. Presence of phasmids, deirids and other morphological characters place Tylenchulidae in a basal position in Criconematoidea.


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