Morpho‐anatomical characterization of some members of euglenophycota (algae) of north‐east Punjab, Pakistan based on light microscopy (LM) and scanning electron microscopy (SEM)

Author(s):  
Nadeem Ullah ◽  
Ghazala Yasmeen Butt ◽  
Mehwish Jaffer ◽  
Quratul Ain
Chromosoma ◽  
2005 ◽  
Vol 115 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Elizabeth Schroeder-Reiter ◽  
Andreas Houben ◽  
Jürke Grau ◽  
Gerhard Wanner

Author(s):  
Reyes Peña-Santiago ◽  
Marcel Ciobanu ◽  
Joaquin Abolafia

Several populations of four known species of the genus Pungentus (P. clavatus, P. engadinensis, P. marietani and P. silvestris), collected in the wild and in cultivated soils from the Iberian Peninsula, are studied. Detailed redescriptions and morphometrics are presented for each species. Illustrations are provided, including line drawings, light microscopy pictures of the four species as well as scanning electron microscopy observations of P. engadinensis. The Iberian populations are compared to type and other known populations, and new data are given that provide a better characterization of these taxa. Pungentus engadinensis is the most widely distributed species in the Iberian Peninsula.


2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Gabriele Patricia Vitoria da Silva ◽  
Bruna Tereza Possamai ◽  
Gabriel da Rosa Schroeder ◽  
Nilton Paulo Vieira Junior ◽  
Enderlei Dec ◽  
...  

Clerodendrum splendens A. Chev., Clerodendrum x speciosum Tiej. & Binn, Clerodendrum thomsonae Balf. F., Clerodendrum ugandense L., Congea tomentosa Roxb., Duranta erecta L., Petrea volubilis L. and Petrea volubilis f. albiflora (Standl.) Standl. pollen grains were acetolyzed, photographed and measured under light microscopy and scanning electron microscopy. Values presented are averages in micrometers. Grains are monads, radially symmetrical, isopolar, large (C. ugandense, very large, C. tomentosa, small-medium and D. erecta, medium),tricolpate (P. volubilis f. albiflora, dimorphic grains with 3-4 colpus). Ambitus is circular (C. tomentosa and D. erecta, sub-circular, P. volubilis , triangular, P. volubilis f. albiflora, triangular-quadrangular). The form is oblate-spheroidal (C. splendens, C. x speciosum, C. ugandense), prolate-spheroidal (C. thomsonae), prolate (C. tomentosa), suboblate (D. erecta) and oblate (P. volubilis, P. volubilis f. albiflora). Exine thickness is in C. splendens 4,28, C. x speciosum 4,19, C. ugandense 4,33, C. thomsonae 4,18, C. tomentosa 1,4, D. erecta 1,55, P. volubilis 2,49, P. volubilis f. albiflora 2,68. Ornamentation is micro-echinate (C. splendens, C. x speciosum, C. thomsonae), echinate (C. ugandense), reticulate (C. tomentosa), psilate (D. erecta, P. volubilis, P. volubulis f. albiflora). Duranta and Petrea are close to Verbenaceae pattern, Congea to Lamiaceae and Clerodendrum loosely to Lamiaceae.


Author(s):  
H.K. Plummer ◽  
W.T. Donlon ◽  
J.E. Allison ◽  
S. Shinozaki

A centrifugally atomized rapidly solidified Al-8Fe-2Mo powder was characterized by Light Microscopy (LM), Scanning Electron Microscopy (SEM) and Analytical Electron Microscopy (AEM). The powder sample was studied in an as-solidified state and after annealing in 1 at. argon for 6 hr at 400°C. Further studies have characterized the consolidated bars produced by compaction and extrusion of these powders.The 30 to 150 μm powders are spherical as seen in the SEM (Fig.1) with small (5 to 15 μm) satellite powders sometimes attached in external (A,B Fig.1) and internal (A Fig.2C) locations. A comparison of powders (epoxy mounted and polished) by both the LM (Fig. 2A) and the SEM (Figs. 2B and 2C) reveals a two component microstructure with occasional voids.


Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).


Author(s):  
D. Johnson ◽  
P. Moriearty

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.


Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.


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