Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Fine structure of the microsymbiont of the actinorhizal root nodules of mountain mahogany (Cercocarpus ledifolius, family Rosaceae)

1989 ◽  
Vol 67 (1) ◽  
pp. 116-120 ◽  
Author(s):  
Susan M. Wood ◽  
William Newcomb ◽  
David Nelson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Root Nodules ◽  
Single Electron ◽  
Transmission Electron ◽  
Cercocarpus Ledifolius ◽  
Scanning Electron

Root nodules of Cercocarpus ledifolius Nutt. (mountain mahogany) were studied by light microscopy, scanning electron microscopy, and transmission electron microscopy to confirm the bacterial nature of the microsymbiont and to determine the morphology of the symbiotic vesicles. The microsymbiont is an actinomycete having two morphologies: septate hyphae (ca. 0.5 μm diam.) and ovoid- or elliptical-shaped nonseptate symbiotic vesicles (2.8 × 3.9 μm). Many of the symbiotic vesicles contain a single, electron-dense ovoid- or spherical-shaped structure, measuring 0.26 μm, whose function is unknown. The actinomycete is surrounded by a capsule that has electron-dense droplets in regions near hyphae. No spores or sporangia were observed in these nodules.

Fine structure and development of the zygospore of Rhizopus sexualis (Smith) Callen

1971 ◽  
Vol 263 (848) ◽  
pp. 71-100 ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Probable Function ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Scanning Electron ◽  
Number Form

The development of the zygospore of Rhizopus sexualis (Smith) Callen was followed by light microscopy, transmission electron microscopy and scanning electron microscopy. Details of all stages, including dissolution of the fusion wall, delimitation of the gametangia by septa, and formation of the complex wall of the zygospore are described and illustrated. Changes in number, form and distribution of the organelles and the behaviour of the nuclei are described. The probable function of the cytoplasmic organelles and the possible mechanisms controlling development are discussed.

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

Colonization of Sphagnum cells by Lyophyllum palustre

1985 ◽  
Vol 63 (4) ◽  
pp. 757-761 ◽  
Author(s):  
E. Untiedt ◽  
K. Müller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Host Cell ◽  
Cell Walls ◽  
Transmission Electron ◽  
Scanning Electron

Lyophyllum palustre (Peck) Singer, a basidiomycete (Tricholomataceae) parasitizing Sphagnum, was examined for points of contact between hyphae and Sphagnum cells with the help of light microscopy, scanning electron microscopy, and transmission electron microscopy. Results indicate that the fungus attacks Sphagnum cells by penetrating cell walls and altering host cell protosplasm. In addition, the formation of additional partitioning cell walls in attacked living Sphagnum cells was observed.

scholarly journals Mitochondria of Human Adrenal Cortex Have Tubular Cristae with Bulbous Tips

2003 ◽  
Vol 88 (4) ◽  
pp. 1903-1906 ◽  
Author(s):  
Alessandro Riva ◽  
Felice Loffredo ◽  
Alessandro Uccheddu ◽  
Francesca Testa Riva ◽  
Bernard Tandler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Adrenal Cortex ◽  
Thin Sections ◽  
Transmission Electron ◽  
Soluble Material ◽  
Bulbous Tip ◽  
Scanning Electron

By taking advantage of a modified osmium maceration technique, we have been able to examine by high resolution scanning electron microscopy (HRSEM) the interior of human adrenocortical mitochondria from which all soluble material has been extracted. The so-called vesicles apparent in thin sections examined by transmission electron microscopy actually are finger-like cristae as determined by HRSEM. These digitiform cristae have a segmented appearance and a bulbous tip. The segmented form of the cristae may have important metabolic implications.

Sperm associations in rat epididymis

1990 ◽  
Vol 48 (3) ◽  
pp. 56-57
Author(s):  
Miguel W. Fomés ◽  
Mario H. Burgos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Guinea Pig ◽  
Light Microscopy ◽  
Salt Solution ◽  
Transmission Electron ◽  
Rat Epididymis ◽  
Cover Slide ◽  
Scanning Electron

During epididymal transit a head to head association of spermatozoa has been described in guinea pig, Loris and marsupials. In guinea pig they are permanent and androgen dependent. Other type of sperm association described in nonseasonal. mammals, adopt the stiape of dense masses of degenerating spermatozoa.In the present study a new transitory head to head association in rat spemiatozoa is described which appears at the end of the epididymal transit and dense masses of associated spennatozoa in degeneration similar to those described in the literature. The sperm samples were obtained by puncture of the 3 main epididymal regions. The emerging fluid drops were immediately suspended in a balanced salt solution, placed in a slide-cover slide chanber at 36°C, and observed and recorded with a video equipment. Other drops of epididymal fluid were fixed in Mollenhauer (1976) and processed for light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

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