A scanning electron microscopic and flame spectrometry study on the role of Ca2+ in amphibian neurulation using papaverine inhibition and ionophore induction of morphogenetic movement

1976 ◽  
Vol 198 (3) ◽  
pp. 409-416 ◽  
Author(s):  
Denis J. Moran
1982 ◽  
Vol 91 (6) ◽  
pp. 612-614 ◽  
Author(s):  
James P. Dudley ◽  
Lance Eisner ◽  
James D. Cherry

When cilia stop beating, their role in moving mucus ceases, but it is uncertain if nonmotile cilia preserve their external architecture and are thus capable of maintaining an additional role of retarding microbial access to the cell. Cilia of chicken embryo tracheal organ cultures were observed until their activity stopped. When examined with scanning electron microscopy, ciliary axonemes did not appear to differ significantly from normally functioning cilia when observed at lower magnifications. Since their density can remain essentially unchanged, nonbeating cilia may still have a role in protecting respiratory membrane from toxic microorganisms.


2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Aris F. Pourlis

The main purpose of this study is to extend the microscopic investigations of the pecten oculi in the quail in order to add some information on the unresolved functional anatomy of this unique avian organ. The pecten oculi of the quail was studied by scanning electron microscopy. Eighteen- to-twenty two highly vascularised accordion-like folds were joined apically by a heavily pigmented bridge of tissue, which holds the pecten in a fanlike shape, widest at the base. The structure of the double layered limiting membrane was recorded. The presence of hyalocytes with macrophage-like appearance was illustrated. It is assumed that the pecten oculi of the quail resembles that of the chicken. Illustrated morphological features of this species may add information on the active physiological role of the pecten. But still, the functional significance of this organ is a matter of controversies.


Author(s):  
Toichiro Kuwabara

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.


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