Gas chromatography of volatile fatty acids astert-butyidimethylsilyl esters

1987 ◽  
Vol 10 (9) ◽  
pp. 522-523 ◽  
Author(s):  
K. Kim ◽  
A. Zlatkis ◽  
E. C. Horning ◽  
B. S. Middleditch
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Volatile Fatty Acids

Simpler liquid-chromatographic screening for organic acid disorders.

1984 ◽  
Vol 30 (4) ◽  
pp. 542-546 ◽  
Author(s):  
M J Bennett ◽  
C E Bradey
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Volatile Fatty Acids ◽  
Simultaneous Detection ◽  
Organic Aciduria ◽  
Gas Chromatography Mass Spectrometry ◽  
Identification System ◽  
Chromatographic Technique ◽  
Normal Infant

Abstract We describe an isocratic cation-exchange chromatographic technique, with ultraviolet detection, for determination of urinary organic acids. The method is useful for screening urines from neonates and infants suspected of having an organic aciduria, prior to confirmatory analysis by gas chromatography-mass spectrometry. Advantages of this technique over conventional gas chromatography include ease of sample preparation and the simultaneous detection of volatile fatty acids; limitations include its lack of an identification system like that of mass spectrometry, the presence of nonultraviolet-absorbing fatty acids, and the relatively long retention times of phenolic compounds. Chromatograms for patients with methylmalonic aciduria, propionic aciduria, isovaleric aciduria, beta-ketothiolase deficiency, lactic aciduria, and glutaric aciduria type II are compared with those for a normal infant.

Application of gas chromatography-mass spectrometry preceded by solvent extraction to determine volatile fatty acids in wastewater of municipal, animal farm and landfill origin

2011 ◽  
Vol 63 (4) ◽  
pp. 590-597 ◽  
Author(s):  
Anna Banel ◽  
Bogdan Zygmunt
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Volatile Fatty Acids ◽  
Municipal Wastewater ◽  
Treated Wastewater ◽  
Methyl Tert Butyl Ether ◽  
Landfill Leachates ◽  
Animal Farms

This study describes extraction of selected volatile fatty acids (VFAs) (containing from 2 up to 8 carbon atoms) with methyl-tert-butyl ether (MTBE) from polluted aqueous samples followed by separation, identification and quantification by gas chromatography coupled with mass spectrometry (GC-MS). Extraction parameters such as time and number of extractions, volume ratio, effect of acidification and salt addition were optimized with respect to recovery, enrichment factor and repeatability. The VFAs studied were separated using an open tubular capillary column Stabilwax-DA (crossbond polyethylene glycol treated with nitroterephtalic acid). The limits of quantitation (LOQ) of the procedure developed were on the level of 0.1 to 0.5 mg L−1. The analysis of real samples of municipal raw and treated wastewater, animal farms wastewater, and also landfill leachates showed that always dominant was acetic acid. The content of VFAs in animal farms wastewater was a few times higher than in municipal wastewater and in municipal solid waste landfill leachates. In surface and ground water close to municipal landfill only acetic acid was detected at higher concentrations in outflow than inflow water. This indicates that slight leakage from the landfill must have occurred.

Quantitative determination of C2-C8 volatile fatty acids in human serum by vacuum distillation and gas chromatography

1983 ◽  
Vol 133 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Albert Tangerman ◽  
Annie van Schaik ◽  
Maria T. Meuwese-Arends ◽  
Jan H.M. van Tongeren
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Quantitative Determination ◽  
Human Serum ◽  
Vacuum Distillation ◽  
Volatile Fatty Acids

Development of headspace–gas chromatography–flame ionization detection procedure to determine volatile fatty acids in zoo organic waste leachates

2011 ◽  
Vol 63 (12) ◽  
pp. 2873-2877 ◽  
Author(s):  
Anna Banel ◽  
Marta Wasielewska ◽  
Monika Felchner-Żwirełło ◽  
Bogdan Zygmunt
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Organic Waste ◽  
Volatile Fatty Acids ◽  
Sample Introduction ◽  
Flame Ionization Detection ◽  
Monocarboxylic Acids ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Headspace Gas

In order to determine volatile fatty acids (short chain monocarboxylic acids with 2 to 6 carbon atoms in a molecule) in leachates formed in organic waste piles a procedure based on static headspace (HS) coupled with gas chromatography (GC) have been proposed and optimized. The conditions of HS extraction and sample introduction into a gas chromatograph as well as of chromatographic separation were considered. Using flame ionization detection LODs were of the order of 0.02– 0.37 mg/L. The analyzed leachate collecting on and close to a zoo organic waste pile contained VFAs (Volatile Fatty Acids) at a concentration ranging from 5.5 mg/L to 0.88 g/L and from below LOD to 10.1 mg/L for pool water at some distance from the pile, respectively.

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